ID   SYL_SHEFN               Reviewed;         863 AA.
AC   Q087K2;
DT   20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT   20-MAY-2008, sequence version 2.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=Sfri_0705;
OS   Shewanella frigidimarina (strain NCIMB 400).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Alteromonadales;
OC   Shewanellaceae; Shewanella.
OX   NCBI_TaxID=318167;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=NCIMB 400;
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Fredrickson J.K., Kolker E., McCuel L.A., DiChristina T., Nealson K.H.,
RA   Newman D., Tiedje J.M., Zhou J., Romine M.F., Culley D.E., Serres M.,
RA   Chertkov O., Brettin T., Bruce D., Han C., Tapia R., Gilna P., Schmutz J.,
RA   Larimer F., Land M., Hauser L., Kyrpides N., Mikhailova N., Richardson P.;
RT   "Complete sequence of Shewanella frigidimarina NCIMB 400.";
RL   Submitted (AUG-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=ABI70563.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR   EMBL; CP000447; ABI70563.1; ALT_INIT; Genomic_DNA.
DR   RefSeq; WP_041413368.1; NC_008345.1.
DR   AlphaFoldDB; Q087K2; -.
DR   SMR; Q087K2; -.
DR   STRING; 318167.Sfri_0705; -.
DR   EnsemblBacteria; ABI70563; ABI70563; Sfri_0705.
DR   KEGG; sfr:Sfri_0705; -.
DR   eggNOG; COG0495; Bacteria.
DR   HOGENOM; CLU_004427_0_0_6; -.
DR   OrthoDB; 32262at2; -.
DR   Proteomes; UP000000684; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 2.
DR   Gene3D; 3.90.740.10; -; 1.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002302; Leu-tRNA-ligase.
DR   InterPro; IPR025709; Leu_tRNA-synth_edit.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR43740; PTHR43740; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 3.
DR   Pfam; PF13603; tRNA-synt_1_2; 1.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00396; leuS_bact; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..863
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_0000334817"
FT   MOTIF           42..52
FT                   /note="'HIGH' region"
FT   MOTIF           622..626
FT                   /note="'KMSKS' region"
FT   BINDING         625
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   863 AA;  97261 MW;  1675C3830AD8D792 CRC64;
     MQELYNPSEI EALVQKHWHE HKTFEVTEDE SKEKFYCLSM FPYPSGRLHM GHVRNYTIGD
     VVARYQRLQG KNVLQPIGWD SFGLPAENAA INNKASPAPW TYENIDYMKN QLKLLGFGYD
     WSREIATCTP EYYRWEQWFF TQLYAKGLVY KKTSSVNWCP NDETVLANEQ VQDGCCWRCD
     TEVIQKEIPQ WFIKITDYAE ELLNDIDTLD GWPEQVKAMQ RNWIGRSEGI EMTFAVADSD
     ASFDIYTTRP DTLMGVTYVA IAAGHPLAEQ SAQNNPELAA FLEECKNADT TEAAMASMEK
     KGVATGLQAI HPISGKLVPI WVANFVLMNY GTGAVMSVPA HDQRDYEFAT KYQLAIEAVI
     KPVDGEVDVS KEAYTEKGVV FNSGDAFPEL DGLDFQAAFE AIDARLTAEG KGKRQVNYRL
     RDWGVSRQRY WGAPIPMVTL ADGTVVPTPE DQLPVILPED VVMDGIQSPI KADKAWAETT
     VNGQPATRET DTFDTFMESS WYYARYCSPH ADEMLDPAKA NYWLPVDQYI GGIEHACMHL
     LYFRFFHKLL RDAGLVNSDE PAKQLLTQGM VLADAYYYTN EKGARVWVSP LEVTVVEKDD
     KGRIIKAIDN QGHELVYTGM SKMSKSKNNG IDPQVMVEKY GADTVRLFMM FASPPELTLE
     WQESGVEGAH RFIKRFWKLA SDHVAAGKTE ALDTSKLNAD QKALRRELHK TIAKVSDDIA
     RRQMFNTAVA SVMELMNHLL KASQETAQDR ALLAEALSAV TRLLYPIVPH MTFTLWNELG
     NEGDIEDSRW PEVDESALVE DSKLIVVQVN GKVRAKITVA ADASKEDVEA LGMSDEHVQK
     HTEGLTVRKV IYVPGKLLSI VAN