ID   BLRF2_EBVA8             Reviewed;         162 AA.
AC   P0C717; Q777F1;
DT   26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   26-MAY-2009, sequence version 1.
DT   23-FEB-2022, entry version 40.
DE   RecName: Full=Tegument protein BLRF2;
GN   ORFNames=BLRF2;
OS   Epstein-Barr virus (strain AG876) (HHV-4) (Human herpesvirus 4).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Gammaherpesvirinae; Lymphocryptovirus.
OX   NCBI_TaxID=82830;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16490228; DOI=10.1016/j.virol.2006.01.015;
RA   Dolan A., Addison C., Gatherer D., Davison A.J., McGeoch D.J.;
RT   "The genome of Epstein-Barr virus type 2 strain AG876.";
RL   Virology 350:164-170(2006).
RN   [2]
RP   FUNCTION, INTERACTION WITH HOST CGAS, AND SUBCELLULAR LOCATION.
RX   PubMed=26320998; DOI=10.1016/j.chom.2015.07.015;
RA   Wu J.J., Li W., Shao Y., Avey D., Fu B., Gillen J., Hand T., Ma S., Liu X.,
RA   Miley W., Konrad A., Neipel F., Stuerzl M., Whitby D., Li H., Zhu F.;
RT   "Inhibition of cGAS DNA Sensing by a Herpesvirus Virion Protein.";
RL   Cell Host Microbe 18:333-344(2015).
CC   -!- FUNCTION: Plays a role in the inhibition of host innate immune system
CC       by targeting the CGAS enzymatic activity which is the principal
CC       cytosolic DNA sensor that detects invading viral DNA (PubMed:26320998).
CC       Acts by inhibiting CGAS-DNA phase separation: directly binds double-
CC       stranded DNA (dsDNA) in a length dependent but sequence independent
CC       manner and is able to form DNA-induced phase separation in infected
CC       cells. DNA phase separation of ORF52 mediates disruption of liquid-like
CC       droplets in which CGAS is activated, thereby preventing CGAS activity
CC       (By similarity). {ECO:0000250|UniProtKB:Q2HR80,
CC       ECO:0000269|PubMed:26320998}.
CC   -!- SUBUNIT: Homooligomer; homooligomerizes and binds double-stranded DNA
CC       (dsDNA) cooperatively (By similarity). Interacts with host CGAS
CC       (PubMed:26320998). {ECO:0000250|UniProtKB:Q2HR80,
CC       ECO:0000269|PubMed:26320998}.
CC   -!- INTERACTION:
CC       P0C717; P0C717: BLRF2; NbExp=2; IntAct=EBI-9644994, EBI-9644994;
CC       P0C717; K9USF9: BMLF1; Xeno; NbExp=2; IntAct=EBI-9644994, EBI-9645143;
CC   -!- SUBCELLULAR LOCATION: Virion tegument {ECO:0000250|UniProtKB:P03197}.
CC       Host cytoplasm {ECO:0000269|PubMed:26320998}.
CC   -!- SIMILARITY: Belongs to the herpesviridae BLRF2 family. {ECO:0000305}.
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DR   EMBL; DQ279927; ABB89241.1; -; Genomic_DNA.
DR   RefSeq; YP_001129461.1; NC_009334.1.
DR   RefSeq; YP_401666.1; NC_007605.1.
DR   SMR; P0C717; -.
DR   IntAct; P0C717; 8.
DR   MINT; P0C717; -.
DR   DNASU; 3783717; -.
DR   GeneID; 3783717; -.
DR   GeneID; 5176224; -.
DR   KEGG; vg:3783717; -.
DR   KEGG; vg:5176224; -.
DR   Proteomes; UP000007639; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0019033; C:viral tegument; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0042802; F:identical protein binding; IPI:IntAct.
DR   GO; GO:0039503; P:suppression by virus of host innate immune response; IEA:UniProtKB-KW.
DR   InterPro; IPR008642; Herpes_BLRF2.
DR   Pfam; PF05812; Herpes_BLRF2; 1.
PE   1: Evidence at protein level;
KW   Coiled coil; DNA-binding; Host cytoplasm; Host-virus interaction;
KW   Inhibition of host innate immune response by virus; Late protein;
KW   Reference proteome; Viral immunoevasion; Virion; Virion tegument.
FT   CHAIN           1..162
FT                   /note="Tegument protein BLRF2"
FT                   /id="PRO_0000376063"
FT   REGION          118..162
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          12..43
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        137..162
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   162 AA;  17687 MW;  E95CD3B969BFE6D8 CRC64;
     MSAPRKVRLP SVKAVDMSME DMAARLARLE SENKALKQQV LRGGACASST SVPSAPVPPP
     EPLTARQREV MITQATGRLA SQAMKKIEDK VRKSVDGVTT RNEMENILQN LTLRIQVSML
     GAKGQPSPGE GTRPRESNDP NATRRARSRS RGREAKKVQI SD