SYL_SORC5
ID SYL_SORC5 Reviewed; 854 AA.
AC A9EYY7;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 05-FEB-2008, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=sce7417;
OS Sorangium cellulosum (strain So ce56) (Polyangium cellulosum (strain So
OS ce56)).
OC Bacteria; Proteobacteria; Deltaproteobacteria; Myxococcales; Sorangiineae;
OC Polyangiaceae; Sorangium.
OX NCBI_TaxID=448385;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=So ce56;
RX PubMed=17965706; DOI=10.1038/nbt1354;
RA Schneiker S., Perlova O., Kaiser O., Gerth K., Alici A., Altmeyer M.O.,
RA Bartels D., Bekel T., Beyer S., Bode E., Bode H.B., Bolten C.J.,
RA Choudhuri J.V., Doss S., Elnakady Y.A., Frank B., Gaigalat L., Goesmann A.,
RA Groeger C., Gross F., Jelsbak L., Jelsbak L., Kalinowski J., Kegler C.,
RA Knauber T., Konietzny S., Kopp M., Krause L., Krug D., Linke B., Mahmud T.,
RA Martinez-Arias R., McHardy A.C., Merai M., Meyer F., Mormann S.,
RA Munoz-Dorado J., Perez J., Pradella S., Rachid S., Raddatz G., Rosenau F.,
RA Rueckert C., Sasse F., Scharfe M., Schuster S.C., Suen G.,
RA Treuner-Lange A., Velicer G.J., Vorholter F.-J., Weissman K.J., Welch R.D.,
RA Wenzel S.C., Whitworth D.E., Wilhelm S., Wittmann C., Bloecker H.,
RA Puehler A., Mueller R.;
RT "Complete genome sequence of the myxobacterium Sorangium cellulosum.";
RL Nat. Biotechnol. 25:1281-1289(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR EMBL; AM746676; CAN97586.1; -; Genomic_DNA.
DR AlphaFoldDB; A9EYY7; -.
DR SMR; A9EYY7; -.
DR STRING; 448385.sce7417; -.
DR PRIDE; A9EYY7; -.
DR EnsemblBacteria; CAN97586; CAN97586; sce7417.
DR KEGG; scl:sce7417; -.
DR eggNOG; COG0495; Bacteria.
DR HOGENOM; CLU_004427_0_0_7; -.
DR OMA; DIDWADV; -.
DR BioCyc; SCEL448385:SCE_RS37990-MON; -.
DR Proteomes; UP000002139; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.620; -; 2.
DR HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR002302; Leu-tRNA-ligase.
DR InterPro; IPR025709; Leu_tRNA-synth_edit.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR PANTHER; PTHR43740; PTHR43740; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR Pfam; PF13603; tRNA-synt_1_2; 1.
DR Pfam; PF09334; tRNA-synt_1g; 1.
DR PRINTS; PR00985; TRNASYNTHLEU.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF50677; SSF50677; 1.
DR TIGRFAMs; TIGR00396; leuS_bact; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT CHAIN 1..854
FT /note="Leucine--tRNA ligase"
FT /id="PRO_0000334822"
FT REGION 1..32
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 69..80
FT /note="'HIGH' region"
FT MOTIF 633..637
FT /note="'KMSKS' region"
FT BINDING 636
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ SEQUENCE 854 AA; 95563 MW; 0D0B4545E599E4D8 CRC64;
MARRDMAAET MDPRASTEPS PNEPREPARY DHAAVEPRWQ RAWEEGETFR AVRSADPARG
KRYVLDMFPY PSGSGLHVGH PEGYTATDIM SRYFRMRGID VLHVMGWDAF GLPAEQHALE
TGTHPADTTA RNIATFKRQL KMLGFSYDWS RELSTTDPRY VRWTQWIFLQ LFKKGLAYQD
EVSVNWCPAL GTVLANEEVI DGKSERGSHP VYRTPLRQWM LRITAYADRL AEDLRLLDWP
EGTVAMQRSW IGRSEGALIT FEVKGWGKGA LSVFTTRPDT LMGVTYVVLA PEHPLTTWLT
SAESGASEPR REAVRAYVAA AAGKSDRERL AAAAREKTGV DTGLVAVHPI TGVEVPIWVA
DYVLGGYGTG AVMAVPGHDE RDFSFARTYG LPIVEVVSPD GSLHDQLEAA YVDPGVAVRS
GEFDGLATEE CKRAVIARLE ALGRGKREVN YKLRDWVFSR QRYWGEPIPI YFPVELADPQ
GDPRKGAAHT IRYDQPIAVD EASLPIELPP LADFRPGDDP AGPLARAVDW RFFQRDGKWY
ARETNTMPQW AGSCWYYLRF LDPQNDAEPF SEAAYDAWMP VDLYVGGAEH GVLHLLYARF
WHKVLYDLGH VKHPEPFAKL VHQGMILGED NEKMSKSRGN VINPDDIVRA HGADVLRMYE
MFMGPLEAVK PWQSGQIQGV VRFRDRVFAT CTRPLSDAMD DATSRQLHRT IKKVTGDIEG
MAFNTAISAM MVFVNHLSSL PSPPREAVLR LILLVSPFAP HLAEELWRLT GHERSLSYEP
WPTYDEAFCV DDVLEIPVQV NGKVRGRVML AKAASEEEAR AAALGLETVA ALAAGKQLKK
FIYVAGKIVN IVVG
