ID   BLRF2_EBVG              Reviewed;         162 AA.
AC   Q3KST5;
DT   26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT   08-NOV-2005, sequence version 1.
DT   23-FEB-2022, entry version 41.
DE   RecName: Full=Tegument protein BLRF2;
GN   ORFNames=BLRF2;
OS   Epstein-Barr virus (strain GD1) (HHV-4) (Human herpesvirus 4).
OC   Viruses; Duplodnaviria; Heunggongvirae; Peploviricota; Herviviricetes;
OC   Herpesvirales; Herpesviridae; Gammaherpesvirinae; Lymphocryptovirus.
OX   NCBI_TaxID=10376;
OH   NCBI_TaxID=9606; Homo sapiens (Human).
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RX   PubMed=16306603; DOI=10.1128/jvi.79.24.15323-15330.2005;
RA   Zeng M.-S., Li D.-J., Liu Q.-L., Song L.-B., Li M.-Z., Zhang R.-H.,
RA   Yu X.-J., Wang H.-M., Ernberg I., Zeng Y.-X.;
RT   "Genomic sequence analysis of Epstein-Barr virus strain GD1 from a
RT   nasopharyngeal carcinoma patient.";
RL   J. Virol. 79:15323-15330(2005).
CC   -!- FUNCTION: Plays a role in the inhibition of host innate immune system
CC       by targeting the CGAS enzymatic activity which is the principal
CC       cytosolic DNA sensor that detects invading viral DNA. Acts by
CC       inhibiting CGAS-DNA phase separation: directly binds double-stranded
CC       DNA (dsDNA) in a length dependent but sequence independent manner and
CC       is able to form DNA-induced phase separation in infected cells. DNA
CC       phase separation of ORF52 mediates disruption of liquid-like droplets
CC       in which CGAS is activated, thereby preventing CGAS activity.
CC       {ECO:0000250|UniProtKB:Q2HR80}.
CC   -!- SUBUNIT: Homooligomer; homooligomerizes and binds double-stranded DNA
CC       (dsDNA) cooperatively (By similarity). Interacts with host CGAS (By
CC       similarity). {ECO:0000250|UniProtKB:Q2HR80}.
CC   -!- INTERACTION:
CC       Q3KST5; Q66542: BNRF1; NbExp=2; IntAct=EBI-2621066, EBI-2621061;
CC   -!- SUBCELLULAR LOCATION: Virion tegument {ECO:0000250|UniProtKB:P03197}.
CC       Host cytoplasm {ECO:0000250|UniProtKB:P0C717}.
CC   -!- SIMILARITY: Belongs to the herpesviridae BLRF2 family. {ECO:0000305}.
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DR   EMBL; AY961628; AAY41115.1; -; Genomic_DNA.
DR   SMR; Q3KST5; -.
DR   IntAct; Q3KST5; 1.
DR   PRIDE; Q3KST5; -.
DR   Proteomes; UP000007641; Genome.
DR   GO; GO:0030430; C:host cell cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0019033; C:viral tegument; IEA:UniProtKB-SubCell.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   GO; GO:0039503; P:suppression by virus of host innate immune response; IEA:UniProtKB-KW.
DR   InterPro; IPR008642; Herpes_BLRF2.
DR   Pfam; PF05812; Herpes_BLRF2; 1.
PE   1: Evidence at protein level;
KW   Coiled coil; DNA-binding; Host cytoplasm; Host-virus interaction;
KW   Inhibition of host innate immune response by virus; Late protein;
KW   Viral immunoevasion; Virion; Virion tegument.
FT   CHAIN           1..162
FT                   /note="Tegument protein BLRF2"
FT                   /id="PRO_0000376064"
FT   REGION          121..162
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          12..43
FT                   /evidence="ECO:0000255"
FT   COMPBIAS        137..162
FT                   /note="Basic and acidic residues"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   162 AA;  17703 MW;  E95CD3B9747E3AD8 CRC64;
     MSAPRKVRLP SVKAVDMSME DMAARLARLE SENKALKQQV LRGGACASST SVPSAPVPPP
     EPLTARQREV MITQATGRLA SQAMKKIEDK VRKSVDGVTT RNEMENILQN LTLRIQVSML
     GAKGQPSPGE GTRLRESNDP NATRRARSRS RGREAKKVQI SD