ID   BLR_ECOLI               Reviewed;          41 AA.
AC   P56976; Q2MB73;
DT   01-DEC-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-DEC-2000, sequence version 1.
DT   03-AUG-2022, entry version 112.
DE   RecName: Full=Divisome-associated membrane protein Blr;
DE   AltName: Full=Beta-lactam resistance protein;
GN   Name=blr; OrderedLocusNames=b4409, JW5963;
OS   Escherichia coli (strain K12).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Enterobacteriaceae; Escherichia.
OX   NCBI_TaxID=83333;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA], IDENTIFICATION, SUBCELLULAR LOCATION,
RP   TOPOLOGY, DISRUPTION PHENOTYPE, AND BLOCKAGE OF N-TERMINUS.
RC   STRAIN=K12;
RX   PubMed=10931331; DOI=10.1046/j.1365-2958.2000.01998.x;
RA   Wong R.S.Y., McMurry L.M., Levy S.B.;
RT   "'Intergenic' blr gene in Escherichia coli encodes a 41-residue membrane
RT   protein affecting intrinsic susceptibility to certain inhibitors of
RT   peptidoglycan synthesis.";
RL   Mol. Microbiol. 37:364-370(2000).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / MG1655 / ATCC 47076;
RX   PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA   Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA   Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA   Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA   Shao Y.;
RT   "The complete genome sequence of Escherichia coli K-12.";
RL   Science 277:1453-1462(1997).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX   PubMed=16738553; DOI=10.1038/msb4100049;
RA   Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA   Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT   "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT   and W3110.";
RL   Mol. Syst. Biol. 2:E1-E5(2006).
RN   [4]
RP   FUNCTION, INTERACTION WITH FTSL; FTSI; FTSK; FTSN; FTSQ; FTSW AND YMGF, AND
RP   SUBCELLULAR LOCATION.
RC   STRAIN=K12;
RX   PubMed=22885295; DOI=10.1128/jb.00774-12;
RA   Karimova G., Davi M., Ladant D.;
RT   "The beta-lactam resistance protein Blr, a small membrane polypeptide, is a
RT   component of the Escherichia coli cell division machinery.";
RL   J. Bacteriol. 194:5576-5588(2012).
CC   -!- FUNCTION: Component of the cell division machinery, which is probably
CC       involved in the stabilization of the divisome under certain stress
CC       conditions. {ECO:0000269|PubMed:22885295}.
CC   -!- SUBUNIT: Interacts with FtsL and several other divisomal proteins,
CC       including FtsI, FtsK, FtsN, FtsQ, FtsW and YmgF.
CC       {ECO:0000269|PubMed:22885295}.
CC   -!- INTERACTION:
CC       P56976; P0AEN4: ftsL; NbExp=3; IntAct=EBI-6419495, EBI-1119082;
CC       P56976; P29131: ftsN; NbExp=3; IntAct=EBI-6419495, EBI-1134233;
CC   -!- SUBCELLULAR LOCATION: Cell inner membrane {ECO:0000269|PubMed:10931331,
CC       ECO:0000269|PubMed:22885295}; Single-pass membrane protein
CC       {ECO:0000269|PubMed:10931331, ECO:0000269|PubMed:22885295}.
CC       Note=Localizes to the division septum. Localization requires FtsQ and
CC       FtsN.
CC   -!- PTM: The N-terminus is blocked.
CC   -!- DISRUPTION PHENOTYPE: Inactivation increases the susceptibility of
CC       bacteria to a number of antibiotics that inhibit peptidoglycan
CC       biosynthesis. Increases the susceptibility to cycloserine, bacitracin,
CC       and a wide spectrum of beta-lactam antibiotics. Does not affect
CC       susceptibility to fosfomycin, valinomycin, tetracycline,
CC       chloramphenicol, gentamicin or quinolones.
CC       {ECO:0000269|PubMed:10931331}.
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DR   EMBL; AF219227; AAF82191.1; -; Genomic_DNA.
DR   EMBL; U00096; AAT48132.1; -; Genomic_DNA.
DR   EMBL; AP009048; BAE76483.1; -; Genomic_DNA.
DR   RefSeq; WP_001300888.1; NZ_STEB01000003.1.
DR   RefSeq; YP_025303.1; NC_000913.3.
DR   AlphaFoldDB; P56976; -.
DR   SMR; P56976; -.
DR   BioGRID; 4259631; 432.
DR   BioGRID; 853435; 1.
DR   IntAct; P56976; 8.
DR   STRING; 511145.b4409; -.
DR   PaxDb; P56976; -.
DR   EnsemblBacteria; AAT48132; AAT48132; b4409.
DR   EnsemblBacteria; BAE76483; BAE76483; BAE76483.
DR   GeneID; 2847682; -.
DR   GeneID; 66674484; -.
DR   KEGG; ecj:JW5963; -.
DR   KEGG; eco:b4409; -.
DR   PATRIC; fig|83333.103.peg.2464; -.
DR   HOGENOM; CLU_185071_0_0_6; -.
DR   BioCyc; EcoCyc:MON0-1561; -.
DR   PRO; PR:P56976; -.
DR   Proteomes; UP000000318; Chromosome.
DR   Proteomes; UP000000625; Chromosome.
DR   GO; GO:0000935; C:division septum; IDA:EcoCyc.
DR   GO; GO:0016021; C:integral component of membrane; IDA:EcoCyc.
DR   GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0007049; P:cell cycle; IEA:UniProtKB-KW.
DR   GO; GO:0051301; P:cell division; IEA:UniProtKB-KW.
DR   GO; GO:0036460; P:cellular response to cell envelope stress; IMP:EcoCyc.
DR   GO; GO:0046677; P:response to antibiotic; IMP:EcoCyc.
PE   1: Evidence at protein level;
KW   Antibiotic resistance; Cell cycle; Cell division; Cell inner membrane;
KW   Cell membrane; Membrane; Reference proteome; Stress response;
KW   Transmembrane; Transmembrane helix.
FT   CHAIN           1..41
FT                   /note="Divisome-associated membrane protein Blr"
FT                   /id="PRO_0000064943"
FT   TOPO_DOM        1..3
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        4..24
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        25..41
FT                   /note="Periplasmic"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   41 AA;  4556 MW;  D384EBB01276D46B CRC64;
     MNRLIELTGW IVLVVSVILL GVASHIDNYQ PPEQSASVQH K