SYL_SYNS3
ID SYL_SYNS3 Reviewed; 867 AA.
AC Q0IAE0;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 03-OCT-2006, sequence version 1.
DT 03-AUG-2022, entry version 108.
DE RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=sync_1375;
OS Synechococcus sp. (strain CC9311).
OC Bacteria; Cyanobacteria; Synechococcales; Synechococcaceae; Synechococcus;
OC unclassified Synechococcus.
OX NCBI_TaxID=64471;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=CC9311;
RX PubMed=16938853; DOI=10.1073/pnas.0602963103;
RA Palenik B., Ren Q., Dupont C.L., Myers G.S., Heidelberg J.F., Badger J.H.,
RA Madupu R., Nelson W.C., Brinkac L.M., Dodson R.J., Durkin A.S.,
RA Daugherty S.C., Sullivan S.A., Khouri H., Mohamoud Y., Halpin R.,
RA Paulsen I.T.;
RT "Genome sequence of Synechococcus CC9311: insights into adaptation to a
RT coastal environment.";
RL Proc. Natl. Acad. Sci. U.S.A. 103:13555-13559(2006).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR EMBL; CP000435; ABI47465.1; -; Genomic_DNA.
DR RefSeq; WP_011619300.1; NC_008319.1.
DR AlphaFoldDB; Q0IAE0; -.
DR SMR; Q0IAE0; -.
DR STRING; 64471.sync_1375; -.
DR EnsemblBacteria; ABI47465; ABI47465; sync_1375.
DR KEGG; syg:sync_1375; -.
DR eggNOG; COG0495; Bacteria.
DR HOGENOM; CLU_004427_0_0_3; -.
DR OMA; TFMVLAP; -.
DR OrthoDB; 32262at2; -.
DR Proteomes; UP000001961; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.620; -; 2.
DR HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR002302; Leu-tRNA-ligase.
DR InterPro; IPR025709; Leu_tRNA-synth_edit.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR PANTHER; PTHR43740; PTHR43740; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 3.
DR Pfam; PF13603; tRNA-synt_1_2; 1.
DR PRINTS; PR00985; TRNASYNTHLEU.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF50677; SSF50677; 1.
DR TIGRFAMs; TIGR00396; leuS_bact; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT CHAIN 1..867
FT /note="Leucine--tRNA ligase"
FT /id="PRO_0000334828"
FT REGION 308..327
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 57..67
FT /note="'HIGH' region"
FT MOTIF 631..635
FT /note="'KMSKS' region"
FT BINDING 634
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ SEQUENCE 867 AA; 96742 MW; 88FE65741507DAF4 CRC64;
MTAFSSPSSN AASSATDRYQ PLALEERWQA LWKEQRLYET QDPKPGQRAF YALSMFPYPS
GTLHMGHVRN YVITDVIARV QRMRGDAVLH PMGWDAFGLP AENAAIERKI EPGVWTDSNI
AQMRGQLGRL GLSIDWDREV ATCHSDYYRW TQWLFLELHA GGLAYQKDAT VNWDPVDQTV
LANEQVDADG RSWRSGALVE KRDLRQWFLR ITDYADALLD DLDQLQGWPE RVRTMQANWI
GRSIGAEIDF QVEAHPGTSI TVFTTRPDTL FGVSYLVLAP DHALVDQLTT SEERISVTAF
RDLMAELSQD ERTSDDQPKR GVPTGAVAIN PANGKSIPIW IADYVLADYG TGAVMGVPAH
DVRDFSFARQ HELPVQRVIE VSGTNEHVND GEAWAGPGTL IHSAGFSGLS NDEAKTAITN
HGAENGWARA KRQYRLRDWL ISRQRYWGCP IPIIHCDDCG AVPVPRDQLP VELPTGIDLK
GAGGSPLARA EDWVSVTCPK CGKPARRETD TMDTFMCSSW YYLRFADPHN RDLPFNATSV
NRWLPVKQYV GGIEHAILHL LYARFFTKAL NDRDLLQTKE PFERLLTQGM VQGTTYRNPR
TGRYISPAAV KDESNPLDPD DGGPLEVLFE KMSKSKHNGV DPAAVIDRYG ADTARMFILF
KAPPEKDLEW DDADVEGQFR FLQRLWRLVD SEVNHDGVSP ATGESDSDIR RAVHQAIKAV
SEDLSDDFQF NTAISELMKL SNSLSSGLAQ ASPGVRQEAM SALVRLLAPF APHLAEEFWQ
RLGGEDSVHC QPWPDHDPEA LVLASIEVVI QVKGKVRGSM SVAADCSKEE LERLALASDV
AQRWLEGKPP RRVIVVPGKL VNLVPSS
