SYL_UREU1
ID SYL_UREU1 Reviewed; 806 AA.
AC B5ZBI3;
DT 24-MAR-2009, integrated into UniProtKB/Swiss-Prot.
DT 25-NOV-2008, sequence version 1.
DT 03-AUG-2022, entry version 86.
DE RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=UUR10_0380;
OS Ureaplasma urealyticum serovar 10 (strain ATCC 33699 / Western).
OC Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Ureaplasma.
OX NCBI_TaxID=565575;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 33699 / Western;
RA Shrivastava S., Methe B.A., Glass J., White K., Duffy L.B.;
RT "Genome sequence of Ureaplasma urealyticum serovar 10 ATCC-33699.";
RL Submitted (OCT-2008) to the EMBL/GenBank/DDBJ databases.
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR EMBL; CP001184; ACI60167.1; -; Genomic_DNA.
DR RefSeq; WP_012560305.1; NC_011374.1.
DR AlphaFoldDB; B5ZBI3; -.
DR SMR; B5ZBI3; -.
DR STRING; 565575.UUR10_0380; -.
DR EnsemblBacteria; ACI60167; ACI60167; UUR10_0380.
DR GeneID; 45015927; -.
DR KEGG; uue:UUR10_0380; -.
DR eggNOG; COG0495; Bacteria.
DR HOGENOM; CLU_004427_0_0_14; -.
DR OMA; TFMVLAP; -.
DR OrthoDB; 32262at2; -.
DR Proteomes; UP000002018; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.620; -; 2.
DR HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR002302; Leu-tRNA-ligase.
DR InterPro; IPR025709; Leu_tRNA-synth_edit.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR PANTHER; PTHR43740; PTHR43740; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR Pfam; PF13603; tRNA-synt_1_2; 1.
DR Pfam; PF09334; tRNA-synt_1g; 1.
DR PRINTS; PR00985; TRNASYNTHLEU.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF50677; SSF50677; 1.
DR TIGRFAMs; TIGR00396; leuS_bact; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis.
FT CHAIN 1..806
FT /note="Leucine--tRNA ligase"
FT /id="PRO_1000091376"
FT MOTIF 40..51
FT /note="'HIGH' region"
FT MOTIF 580..584
FT /note="'KMSKS' region"
FT BINDING 583
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ SEQUENCE 806 AA; 93235 MW; F067C46230A60CBB CRC64;
MYNHNKIEKK WQKYWLDNKT FKFVDNPNNP KKFYVLDMFP YPSGKGLHVG HPKGYTATDV
ISRFKRLNGY DVLHPIGWDA FGLPAEQYAL ETNNHPHTFT QQNIKIFRKQ LQMIGFDFDY
DKEVDTTDPQ FYQWTQWIFV QLYKHNLAEI QDIDVNWCEN LGTVLSNEEV VLNDKNERVS
ERGGHPVVRK PMKQWVLKIV DYADKLLDGL NEVEFSESLK SLQRNWIGKS IGTNVQFKIK
DSHLALDVFT TRIDTIYGAQ YLVVAPEHPI LKSIVSEQQA SVVQAYVDQT KKISDLDRIA
DTNKTGVFSG TYAINPINQE IIPIWVSDYV LMNFATGAVM GVPAHDERDY AFAKKYDLPI
KSVIDTKQSL PYTGDGLHIN SPMINGLNIE QSQNILNDYL VKNHLAKRVV NYKLRNWIFS
RQRYWGEPFP VLFDENNQIK IIEDLPVLLP NLDEFKPSKT GESPLANAQE WLYVEIDGKK
YRRETNTMPQ WAGSSWYFLA YILKNEDGSY TPLNSEEAKK RFAKWLPVDV YIGGQEHAVL
HLLYARFWHR FLYDIGVVPT KEPFYKVINQ GMILGENNEK MSKSKGNVIN PDDIIASHGA
DTLRIYEMFM GPLTASLPWS PDGLDAMRKW LDRVYRLYHN LSELEVVEDV NKLNEEIIIT
YHTLIKNYTK AINEQAFNIA ISEMMVFVNV LYKNKVINYK LLDNFLILLS CFAPHLAEEL
YSLNHSESVC LQKMPIYDEQ KIIAQNVTIP IQINGKLKHT INVLRDTNAE QLINLALACE
QVKQAIGDQP IKKQIVVVNK IINFVI