ID   SYL_VEREI               Reviewed;         907 AA.
AC   A1WGU0;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   06-FEB-2007, sequence version 1.
DT   03-AUG-2022, entry version 95.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=Veis_1071;
OS   Verminephrobacter eiseniae (strain EF01-2).
OC   Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales;
OC   Comamonadaceae; Verminephrobacter.
OX   NCBI_TaxID=391735;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=EF01-2;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Dalin E., Tice H., Pitluck S., Chertkov O., Brettin T.,
RA   Bruce D., Han C., Tapia R., Gilna P., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Kim E., Stahl D., Richardson P.;
RT   "Complete sequence of chromosome 1 of Verminephrobacter eiseniae EF01-2.";
RL   Submitted (DEC-2006) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR   EMBL; CP000542; ABM56847.1; -; Genomic_DNA.
DR   RefSeq; WP_011808858.1; NC_008786.1.
DR   AlphaFoldDB; A1WGU0; -.
DR   SMR; A1WGU0; -.
DR   STRING; 391735.Veis_1071; -.
DR   EnsemblBacteria; ABM56847; ABM56847; Veis_1071.
DR   KEGG; vei:Veis_1071; -.
DR   eggNOG; COG0495; Bacteria.
DR   HOGENOM; CLU_004427_0_0_4; -.
DR   OMA; TFMVLAP; -.
DR   OrthoDB; 32262at2; -.
DR   Proteomes; UP000000374; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 2.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002302; Leu-tRNA-ligase.
DR   InterPro; IPR025709; Leu_tRNA-synth_edit.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR43740; PTHR43740; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 1.
DR   Pfam; PF13603; tRNA-synt_1_2; 1.
DR   Pfam; PF09334; tRNA-synt_1g; 1.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00396; leuS_bact; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..907
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_1000009460"
FT   MOTIF           42..52
FT                   /note="'HIGH' region"
FT   MOTIF           651..655
FT                   /note="'KMSKS' region"
FT   BINDING         654
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   907 AA;  101177 MW;  2515C1206D9A9CB4 CRC64;
     MQDKYSPQDI ERAAQDDWRA RDAYRVTQDA GKKKFYACSM LPYPSGKLHM GHVRNYTIND
     MLTRYLRMNG YNVLMPMGWD AFGLPAENAA IKNGMPPARW TRENIAYMKQ QMQALGLAID
     WSRELATCDP GYYKWNQWLF LKMLDKGIAY RKTQVVNWDP VDQTVLANEQ VNEGKGWRTG
     AVVEKREIPG YYLKITDYAE ELLAFVTDDR LPGWPERVKL MQENWIGKSE GLRFAFPHDV
     RGDDGALIGG GRLYVFTTRA DTIMGVTFCA VAPEHPLASH AAKSNPALAA FIDECRSGGT
     TEAALATQEK KGLRTGLYVT HPLTDEPLEV WVGNYVLMGY GDGAVMGVPA HDERDFAFAL
     KYGIEIRQVV LVDGEHFDYR RWQDWYGDKQ RGVTINSDSF SGLHHKEAVQ AVAHVLEQKG
     LGEKKTTWRL RDWGVSRQRY WGTPIPIIHC DQHGAVPVPE KDLPVVLPQD CIPDGTGNPL
     HSHQGFHAGV TCPVCGKSAR RETDTMDTFV DSAWYFMRYC DPKNDQAMVA EGTDYWMRDP
     QQATGGSGMD QYIGGIEHAI LHLLYARFWT KVMRDLGLVK VDEPFNRLLT QGMVLNHIYS
     RRNDNGGREY FWPQDVEQVH DAAGKIIGAR LIRAVGDWPA GSAIDYEGMG TMSKSRNNGV
     DPQELIGKYG ADTARLHTMF TAPPEAALEW NDAAVEGSHR FLRRVWNFGL QLHAAGMTAA
     SASLAGAGAP QTRNPPAFGK PAKALRREIH QLLRQVDYDY QRMQYNTVVS GAMKMLNALE
     NFKASDPASQ ASDPLALTEG FGILLRCLYP VTPHIAHSLW RDLGYAAAVG ELLDAPWPQV
     DGQALVQDEI ELMLQINGKL RGSILVPAQA SQAAIERIAR ASPAALAAGA VPKRVIVVPG
     RLVNLVF