SYL_VESOH
ID SYL_VESOH Reviewed; 815 AA.
AC A5CY09;
DT 15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT 12-JUN-2007, sequence version 1.
DT 03-AUG-2022, entry version 93.
DE RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=COSY_0005;
OS Vesicomyosocius okutanii subsp. Calyptogena okutanii (strain HA).
OC Bacteria; Proteobacteria; Gammaproteobacteria; sulfur-oxidizing symbionts;
OC Candidatus Vesicomyosocius.
OX NCBI_TaxID=412965;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=HA;
RX PubMed=17493812; DOI=10.1016/j.cub.2007.04.039;
RA Kuwahara H., Yoshida T., Takaki Y., Shimamura S., Nishi S., Harada M.,
RA Matsuyama K., Takishita K., Kawato M., Uematsu K., Fujiwara Y., Sato T.,
RA Kato C., Kitagawa M., Kato I., Maruyama T.;
RT "Reduced genome of the thioautotrophic intracellular symbiont in a deep-sea
RT clam, Calyptogena okutanii.";
RL Curr. Biol. 17:881-886(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR EMBL; AP009247; BAF61144.1; -; Genomic_DNA.
DR RefSeq; WP_011929414.1; NC_009465.1.
DR AlphaFoldDB; A5CY09; -.
DR SMR; A5CY09; -.
DR STRING; 412965.COSY_0005; -.
DR PRIDE; A5CY09; -.
DR EnsemblBacteria; BAF61144; BAF61144; COSY_0005.
DR KEGG; vok:COSY_0005; -.
DR eggNOG; COG0495; Bacteria.
DR HOGENOM; CLU_004427_0_0_6; -.
DR OMA; TFMVLAP; -.
DR OrthoDB; 32262at2; -.
DR Proteomes; UP000000247; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.620; -; 2.
DR HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR002302; Leu-tRNA-ligase.
DR InterPro; IPR025709; Leu_tRNA-synth_edit.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR PANTHER; PTHR43740; PTHR43740; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 2.
DR Pfam; PF13603; tRNA-synt_1_2; 1.
DR PRINTS; PR00985; TRNASYNTHLEU.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF50677; SSF50677; 1.
DR TIGRFAMs; TIGR00396; leuS_bact; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis.
FT CHAIN 1..815
FT /note="Leucine--tRNA ligase"
FT /id="PRO_1000009461"
FT MOTIF 42..52
FT /note="'HIGH' region"
FT MOTIF 571..575
FT /note="'KMSKS' region"
FT BINDING 574
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ SEQUENCE 815 AA; 95149 MW; 98F196937EC5C85E CRC64;
MNIKYDVQKI EKQAQKYWKE KKSFEVIEDY SKEKYYCLSM FPYPSGRLHM GHVRNYSIGD
VISRFQRMQG KNVMQPIGWD GFGLPAENAA LKNKESPAKW TYKNINYMKT QLNQLGFGYD
WAREITTCHP KYYRWEQWLF IKLFKKNLIY KKKAIVNWDP VDQTVLANEQ VINGRGWRSN
ALIEKKEISQ WFIRITNYAE ELLNDLDKLY GWPDPVKIMQ KNWIGKSIGL EITFSRRNSD
PLIIYTTRPD TLMGVTYLAI SFEHPLALES GKNNYQVQSF IEKCKTIQTS EILNEIMDKK
GIDSGFKCIH PITNNEVPIW ITNFVLMSYG TGAIMSVPAH DKRDFKFAKK YGIFIKQVIN
KNESIDKGPI INKGKLFNSE EFSGMDFNQA YESIAKTLIE KNLGNKKINY RLRDWGISRQ
RYWGCPIPIV NCKYCGSVTV KVKDLPVILP EKVKFYDVSS PIKKMPNFYQ TICPKCGSKA
HRETDTFDTF FESSWYFARH TCNNNNNAML DKRVNYWLEV DQYIGGVEHS ILHLLYARFF
NKLLRDEGLI KYDEPFKNLL TQGMVLKNGV KMSKSKGNTV DPTKMIKKYG ADTVRLFILF
AAPPMQDLEW NNSGLEGAHR FIKKVYRLVS IYINDSKDYI VNHLNINFLN KTQKHIRRKI
HQNLVKITDD INRRYTFNTA ISTLMESVDI INKFTKTDTQ SIALRSESIN IILLTLSPIT
PHICHYLWLK LGNKKAIINE PWPKVDLKAL IESEVQIIIQ VDGKLRDKMM MMINTDKEIL
ESEVLSNKNI IKFTKNKNII KIIIIHNKLI NIVTK
