SYL_VIBC3
ID SYL_VIBC3 Reviewed; 858 AA.
AC A5F2X0; C3LYW7;
DT 20-MAY-2008, integrated into UniProtKB/Swiss-Prot.
DT 20-MAY-2008, sequence version 2.
DT 03-AUG-2022, entry version 94.
DE RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049};
GN OrderedLocusNames=VC0395_A0478, VC395_0971;
OS Vibrio cholerae serotype O1 (strain ATCC 39541 / Classical Ogawa 395 /
OS O395).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=345073;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RA Heidelberg J.;
RL Submitted (MAR-2007) to the EMBL/GenBank/DDBJ databases.
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39541 / Classical Ogawa 395 / O395;
RX PubMed=19115014; DOI=10.1371/journal.pone.0004053;
RA Feng L., Reeves P.R., Lan R., Ren Y., Gao C., Zhou Z., Ren Y., Cheng J.,
RA Wang W., Wang J., Qian W., Li D., Wang L.;
RT "A recalibrated molecular clock and independent origins for the cholera
RT pandemic clones.";
RL PLoS ONE 3:E4053-E4053(2008).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00049}.
CC -!- SEQUENCE CAUTION:
CC Sequence=ABQ20041.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC Sequence=ACP08983.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; CP000627; ABQ20041.1; ALT_INIT; Genomic_DNA.
DR EMBL; CP001235; ACP08983.1; ALT_INIT; Genomic_DNA.
DR RefSeq; WP_001157869.1; NZ_JAACZH010000005.1.
DR AlphaFoldDB; A5F2X0; -.
DR SMR; A5F2X0; -.
DR STRING; 345073.VC395_0971; -.
DR EnsemblBacteria; ABQ20041; ABQ20041; VC0395_A0478.
DR KEGG; vco:VC0395_A0478; -.
DR KEGG; vcr:VC395_0971; -.
DR PATRIC; fig|345073.21.peg.941; -.
DR eggNOG; COG0495; Bacteria.
DR HOGENOM; CLU_004427_0_0_6; -.
DR Proteomes; UP000000249; Chromosome 2.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR Gene3D; 3.40.50.620; -; 2.
DR HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR002302; Leu-tRNA-ligase.
DR InterPro; IPR025709; Leu_tRNA-synth_edit.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR PANTHER; PTHR43740; PTHR43740; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 3.
DR Pfam; PF13603; tRNA-synt_1_2; 1.
DR PRINTS; PR00985; TRNASYNTHLEU.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF50677; SSF50677; 1.
DR TIGRFAMs; TIGR00396; leuS_bact; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis.
FT CHAIN 1..858
FT /note="Leucine--tRNA ligase"
FT /id="PRO_0000334836"
FT MOTIF 42..52
FT /note="'HIGH' region"
FT MOTIF 618..622
FT /note="'KMSKS' region"
FT BINDING 621
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ SEQUENCE 858 AA; 96647 MW; 51DA349A099B083C CRC64;
MQEQYNPQDI EHKVQQHWDN NKTFVVSEDP NKEKFYCLSM FPYPSGRLHM GHVRNYTIGD
VVSRFQRLQG KNVLQPIGWD AFGLPAENAA VKNNTAPAPW TYENIEYMKN QLKLLGFGYD
WNREFATCRP EYYRWEQEFF TKLFAKGLVY KKTSSVNWCP NDQTVLANEQ VEDGCCWRCD
TPVEQKEIPQ WFIKITAYAQ ELLDDLDNLD GWPEMVKTMQ RNWIGRSEGV ELKFAVKGEN
TDLEVYTTRP DTLMGVTYVG IAAGHPLATK AAANNPALAA FIDECKNTKV AEAEIATMEK
KGMATGLTAI HPLNGREVPI YIANFVLMDY GTGAVMAVPA HDQRDFEFAT KYGLDIIPVI
KPADGSELDV SEAAYTEKGV LFASGEFDGL DFQAAFNAIA AKLEAEGKGK KTVNFRLRDW
GVSRQRYWGA PIPMVTTEDG QVHPVPADQL PVILPEDVVM DGVTSPIKAD KEWAKTTFNG
EPALRETDTF DTFMESSWYY ARYCSPQADD ILDPEKANYW LPVDQYIGGI EHACMHLLYS
RFFYKLLRDA GYVKSDEPFK KLLCQGMVLA DAFYYTNDKG GKEWVSPTEV KVERDGKGRI
VSAVDATGRQ VEHSGMIKMS KSKNNGIDPQ EMVDKYGADT VRLFMMFASP ADMTLEWQES
GVEGANRFLR RVWKLVREHT ELGQAPALDA SALNADQKAL RRDVHKTIAK VTDDVARRQT
FNTAIAAIME LMNKLTKAPM TEAQDRAILD EALKAITLML YPITPHICFE MWVALGQSNI
DTASWPTYDE AALVEDEKLI VLQVNGKLRG KLTVAADATQ QQVEALGMQD ENVQKFIDGL
TVRKVIYVPG KLLNIVAN
