SYL_VIBCH
ID SYL_VIBCH Reviewed; 858 AA.
AC Q9KTE6;
DT 01-JUN-2001, integrated into UniProtKB/Swiss-Prot.
DT 01-JUN-2001, sequence version 2.
DT 03-AUG-2022, entry version 127.
DE RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=VC_0956;
OS Vibrio cholerae serotype O1 (strain ATCC 39315 / El Tor Inaba N16961).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC Vibrio.
OX NCBI_TaxID=243277;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 39315 / El Tor Inaba N16961;
RX PubMed=10952301; DOI=10.1038/35020000;
RA Heidelberg J.F., Eisen J.A., Nelson W.C., Clayton R.A., Gwinn M.L.,
RA Dodson R.J., Haft D.H., Hickey E.K., Peterson J.D., Umayam L.A., Gill S.R.,
RA Nelson K.E., Read T.D., Tettelin H., Richardson D.L., Ermolaeva M.D.,
RA Vamathevan J.J., Bass S., Qin H., Dragoi I., Sellers P., McDonald L.A.,
RA Utterback T.R., Fleischmann R.D., Nierman W.C., White O., Salzberg S.L.,
RA Smith H.O., Colwell R.R., Mekalanos J.J., Venter J.C., Fraser C.M.;
RT "DNA sequence of both chromosomes of the cholera pathogen Vibrio
RT cholerae.";
RL Nature 406:477-483(2000).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00049}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAF94118.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AE003852; AAF94118.1; ALT_INIT; Genomic_DNA.
DR PIR; G82260; G82260.
DR RefSeq; NP_230603.2; NC_002505.1.
DR RefSeq; WP_001157864.1; NZ_LT906614.1.
DR AlphaFoldDB; Q9KTE6; -.
DR SMR; Q9KTE6; -.
DR STRING; 243277.VC_0956; -.
DR EnsemblBacteria; AAF94118; AAF94118; VC_0956.
DR GeneID; 57739645; -.
DR KEGG; vch:VC_0956; -.
DR PATRIC; fig|243277.26.peg.910; -.
DR eggNOG; COG0495; Bacteria.
DR HOGENOM; CLU_004427_0_0_6; -.
DR OMA; TFMVLAP; -.
DR Proteomes; UP000000584; Chromosome 1.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004823; F:leucine-tRNA ligase activity; IBA:GO_Central.
DR GO; GO:0006429; P:leucyl-tRNA aminoacylation; IBA:GO_Central.
DR Gene3D; 3.40.50.620; -; 2.
DR HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR002302; Leu-tRNA-ligase.
DR InterPro; IPR025709; Leu_tRNA-synth_edit.
DR InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR PANTHER; PTHR43740; PTHR43740; 1.
DR Pfam; PF08264; Anticodon_1; 1.
DR Pfam; PF00133; tRNA-synt_1; 3.
DR Pfam; PF13603; tRNA-synt_1_2; 1.
DR PRINTS; PR00985; TRNASYNTHLEU.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF50677; SSF50677; 1.
DR TIGRFAMs; TIGR00396; leuS_bact; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT CHAIN 1..858
FT /note="Leucine--tRNA ligase"
FT /id="PRO_0000152113"
FT MOTIF 42..52
FT /note="'HIGH' region"
FT MOTIF 618..622
FT /note="'KMSKS' region"
FT BINDING 621
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ SEQUENCE 858 AA; 96621 MW; A4D59A2E1C9F1292 CRC64;
MQEQYNPQDI EHKVQQHWDN NKTFVVSEDP NKEKFYCLSM FPYPSGRLHM GHVRNYTIGD
VVSRFQRLQG KNVLQPIGWD AFGLPAENAA VKNNTAPAPW TYENIEYMKN QLKLLGFGYD
WNREFATCRP EYYRWEQEFF TKLFAKGLVY KKTSSVNWCP NDQTVLANEQ VEDGCCWRCD
TPVEQKEIPQ WFIKITAYAQ ELLDDLDNLD GWPEMVKTMQ RNWIGRSEGV ELKFAVKGEN
TDLEVYTTRP DTLMGVTYVG IAAGHPLATK AAANNPALAA FIDECKNTKV AEAEIATMEK
KGMATGLTAI HPLNGREVPI YIANFVLMDY GTGAVMAVPA HDQRDFEFAT KYGLDIIPVI
KPADGSELDV SEAAYTEKGV LFASGEFDGL DFQAAFNAIA AKLEAEGKGK KTVNFRLRDW
GVSRQRYWGA PIPMVTTEDG QVHPVPADQL PVILPEDVVM DGVTSPIKAD KEWAKTTFNG
EPALRETDTF DTFMESSWYY ARYCSPQADD ILDPEKANYW LPVDQYIGGI EHACMHLLYS
RFFHKLLRDA GYVKSDEPFK KLLCQGMVLA DAFYYTNDKG GKEWVSPTEV KVERDGKGRI
VSAVDATGRQ VEHSGMIKMS KSKNNGIDPQ EMVDKYGADT VRLFMMFASP ADMTLEWQES
GVEGANRFLR RVWKLVREHT ELGQAPALDA SALNADQKAL RRDVHKTIAK VTDDVARRQT
FNTAIAAIME LMNKLTKAPM TEAQDRAILD EALKAITLML YPITPHICFE MWVALGQSNI
DTASWPTYDE AALVEDEKLI VLQVNGKLRG KLTVAADATQ QQVEALGMQD ENVQKFIDGL
TVRKVIYVPG KLLNIVAN
