ID   SYL_VIBPA               Reviewed;         857 AA.
AC   Q87RQ0;
DT   23-MAY-2003, integrated into UniProtKB/Swiss-Prot.
DT   23-MAY-2003, sequence version 1.
DT   03-AUG-2022, entry version 116.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=VP0727;
OS   Vibrio parahaemolyticus serotype O3:K6 (strain RIMD 2210633).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Vibrionales; Vibrionaceae;
OC   Vibrio.
OX   NCBI_TaxID=223926;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=RIMD 2210633;
RX   PubMed=12620739; DOI=10.1016/s0140-6736(03)12659-1;
RA   Makino K., Oshima K., Kurokawa K., Yokoyama K., Uda T., Tagomori K.,
RA   Iijima Y., Najima M., Nakano M., Yamashita A., Kubota Y., Kimura S.,
RA   Yasunaga T., Honda T., Shinagawa H., Hattori M., Iida T.;
RT   "Genome sequence of Vibrio parahaemolyticus: a pathogenic mechanism
RT   distinct from that of V. cholerae.";
RL   Lancet 361:743-749(2003).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR   EMBL; BA000031; BAC58990.1; -; Genomic_DNA.
DR   RefSeq; NP_797106.1; NC_004603.1.
DR   RefSeq; WP_005454506.1; NC_004603.1.
DR   AlphaFoldDB; Q87RQ0; -.
DR   SMR; Q87RQ0; -.
DR   STRING; 223926.28805713; -.
DR   EnsemblBacteria; BAC58990; BAC58990; BAC58990.
DR   GeneID; 1188202; -.
DR   KEGG; vpa:VP0727; -.
DR   PATRIC; fig|223926.6.peg.696; -.
DR   eggNOG; COG0495; Bacteria.
DR   HOGENOM; CLU_004427_0_0_6; -.
DR   OMA; TFMVLAP; -.
DR   Proteomes; UP000002493; Chromosome 1.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 2.
DR   Gene3D; 3.90.740.10; -; 1.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002302; Leu-tRNA-ligase.
DR   InterPro; IPR025709; Leu_tRNA-synth_edit.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR43740; PTHR43740; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 3.
DR   Pfam; PF13603; tRNA-synt_1_2; 1.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00396; leuS_bact; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..857
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_0000152114"
FT   MOTIF           42..52
FT                   /note="'HIGH' region"
FT   MOTIF           617..621
FT                   /note="'KMSKS' region"
FT   BINDING         620
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   857 AA;  96654 MW;  D84628AA1E5EE5EB CRC64;
     MQEQYNPQDI EQKVQKHWDD NKTFVVSEDP NKEKFYCLSM FPYPSGRLHM GHVRNYTIGD
     VVSRFQRLQG KNVMQPIGWD AFGLPAENAA VKNNTAPAPW TYENIEYMKN QLKLLGFGYD
     WNREFATCTP EYYRWEQEFF TKLYEKGLVY KKTSSVNWCP NDQTVLANEQ VEDGCCWRCD
     TPVEQKEIPQ WFIKITEYAQ ELLDDLDKLE GWPEMVKTMQ RNWIGRSEGV ELKFEVKGQQ
     DLEVYTTRPD TLMGVTYVGI AAGHPLATLA AENNPELAAF IEECKNTKVA EAELATMEKK
     GMATGLTAIH PLNGREVPVY VANFVLMDYG TGAVMAVPAH DQRDFEFATK YGLDIIPVIK
     PADGSELDIS EAAYTEKGVL FDSGEFDGLE FQAAFDAIAA KLEAEGKGTK TVNFRLRDWG
     VSRQRYWGAP IPMVTTEDGE VHPVPADQLP VILPEDVVMD GVTSPIKADK EWAKTTFNGE
     PALRETDTFD TFMESSWYYA RYCSPQADDI LDPEKANYWL PVDQYIGGIE HACMHLLYSR
     FFHKLLRDAG YVTSDEPFKQ LLCQGMVLAD AFYFENEKGG KEWVAPTDVA VERDGKGRII
     SAKDNEGRDV THSGMIKMSK SKNNGIDPQE MVDKYGADTV RLFMMFASPA DMTLEWQESG
     VEGANRFLKR VWKLVKEHAE KGAAEAVDTA ALSGEQKALR RDVHKTIAKV TDDIARRQTF
     NTAIAAIMEL MNKLAKAPQE SAQDRAILDE ALKAVVTMLY PITPHISYEL WTALGESDID
     NAAWPTFDEK ALVEDEKTIV VQVNGKLRAK LTVAADATKE QVEELGLNDE NVTKFTDGLT
     IRKVIYVPGK LLNIVAN