ID   SYL_YERPN               Reviewed;         860 AA.
AC   Q1CKQ4; C4GR28;
DT   15-JAN-2008, integrated into UniProtKB/Swiss-Prot.
DT   11-JUL-2006, sequence version 1.
DT   03-AUG-2022, entry version 106.
DE   RecName: Full=Leucine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            EC=6.1.1.4 {ECO:0000255|HAMAP-Rule:MF_00049};
DE   AltName: Full=Leucyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00049};
DE            Short=LeuRS {ECO:0000255|HAMAP-Rule:MF_00049};
GN   Name=leuS {ECO:0000255|HAMAP-Rule:MF_00049}; OrderedLocusNames=YPN_1095;
GN   ORFNames=YP516_1189;
OS   Yersinia pestis bv. Antiqua (strain Nepal516).
OC   Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC   Yersiniaceae; Yersinia.
OX   NCBI_TaxID=377628;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Nepal516;
RX   PubMed=16740952; DOI=10.1128/jb.00124-06;
RA   Chain P.S.G., Hu P., Malfatti S.A., Radnedge L., Larimer F., Vergez L.M.,
RA   Worsham P., Chu M.C., Andersen G.L.;
RT   "Complete genome sequence of Yersinia pestis strains Antiqua and Nepal516:
RT   evidence of gene reduction in an emerging pathogen.";
RL   J. Bacteriol. 188:4453-4463(2006).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Nepal516;
RA   Plunkett G. III, Anderson B.D., Baumler D.J., Burland V., Cabot E.L.,
RA   Glasner J.D., Mau B., Neeno-Eckwall E., Perna N.T., Munk A.C., Tapia R.,
RA   Green L.D., Rogers Y.C., Detter J.C., Bruce D.C., Brettin T.S.;
RT   "Yersinia pestis Nepal516A whole genome shotgun sequencing project.";
RL   Submitted (APR-2009) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-
CC         tRNA(Leu); Xref=Rhea:RHEA:11688, Rhea:RHEA-COMP:9613, Rhea:RHEA-
CC         COMP:9622, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:57427,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78494, ChEBI:CHEBI:456215; EC=6.1.1.4;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00049};
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00049}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00049}.
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DR   EMBL; CP000305; ABG17426.1; -; Genomic_DNA.
DR   EMBL; ACNQ01000008; EEO77519.1; -; Genomic_DNA.
DR   RefSeq; WP_002210333.1; NZ_ACNQ01000008.1.
DR   AlphaFoldDB; Q1CKQ4; -.
DR   SMR; Q1CKQ4; -.
DR   EnsemblBacteria; ABG17426; ABG17426; YPN_1095.
DR   GeneID; 66842473; -.
DR   KEGG; ypn:YPN_1095; -.
DR   HOGENOM; CLU_004427_0_0_6; -.
DR   OMA; TFMVLAP; -.
DR   Proteomes; UP000008936; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004823; F:leucine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006429; P:leucyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.620; -; 2.
DR   Gene3D; 3.90.740.10; -; 1.
DR   HAMAP; MF_00049_B; Leu_tRNA_synth_B; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR002300; aa-tRNA-synth_Ia.
DR   InterPro; IPR002302; Leu-tRNA-ligase.
DR   InterPro; IPR025709; Leu_tRNA-synth_edit.
DR   InterPro; IPR013155; M/V/L/I-tRNA-synth_anticd-bd.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   InterPro; IPR009008; Val/Leu/Ile-tRNA-synth_edit.
DR   PANTHER; PTHR43740; PTHR43740; 1.
DR   Pfam; PF08264; Anticodon_1; 1.
DR   Pfam; PF00133; tRNA-synt_1; 3.
DR   Pfam; PF13603; tRNA-synt_1_2; 1.
DR   PRINTS; PR00985; TRNASYNTHLEU.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF50677; SSF50677; 1.
DR   TIGRFAMs; TIGR00396; leuS_bact; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis.
FT   CHAIN           1..860
FT                   /note="Leucine--tRNA ligase"
FT                   /id="PRO_1000009469"
FT   MOTIF           42..52
FT                   /note="'HIGH' region"
FT   MOTIF           619..623
FT                   /note="'KMSKS' region"
FT   BINDING         622
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000255|HAMAP-Rule:MF_00049"
SQ   SEQUENCE   860 AA;  97051 MW;  34DA41D2A01FD153 CRC64;
     MQEQYRPEDI ETQVQLHWQE KQTFKVTEDA SKEKYYCLSM LPYPSGRLHM GHVRNYTIGD
     VISRYQRMLG KNVLQPIGWD AFGLPAEGAA VKNNTAPAPW TYDNIEYMKN QLKLLGFGYD
     WDREIATCKP DYYRWEQWFF TKLYEKGMVY KKTSAVNWCP HDLTVLANEQ VIDGCCWRCD
     TKVERKEIPQ WFIKITDYAE QLLNDLDTLE SWPEQVKTMQ RNWIGRSEGV DIVFDVVDSE
     EKLSVYTTRP DTFMGVTYVA VAAGHPLSLQ AAATNPALAD FVAECRNTKV AEAEMATMEK
     KGMATGLYAI HPLTGEKLPI WAANFVLMDY GTGAVMAVPG HDARDWEFAT KYNLPIKPVI
     LAADGSEPDL SQEAMTEKGT LFNSGEFDGL NHEDGFNAIA DKLVALGVGQ RKVNYRLRDW
     GVSRQRYWGA PIPMVTLEDG TVVPTPEDQL PVILPEDVVM DGISSPIKAD PEWAKTTVNG
     IPGLRETDTF DTFMESSWYY ARYTCPQYDD GMLDPAAANY WLPVDQYVGG IEHAIMHLMY
     FRFFHKLLRD AGLVDSDEPA KRLLCQGMVL ADAFYYTGNN GERIWVSPVD AIVERDDKGR
     IVKAVDAEGH ELVYAGMSKM SKSKNNGIDP QVMVEKYGAD TVRLFMMFAS PAEMTLEWQE
     SGVEGANRFL KRVWRLAFDH TAKGAVKPLD IASLTEEQKS LRRDLHKTIA KVTDDVGRRQ
     TFNTAIAAVM ELMNKLGRAP QETEQDRALM QEALLAVVRM LYPFTPHVCF SLWQALGGEG
     DIDTAPWPIA DEQAMVEDSK LVVVQVNGKV RGRITVPADA TEQQVRERAG QEHLVAKYLD
     GVTVRKVIYV PGKLLNLVVG