SYM1_YEAST
ID SYM1_YEAST Reviewed; 197 AA.
AC Q06563; D6VYP9;
DT 16-MAY-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-NOV-1996, sequence version 1.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Protein SYM1;
DE AltName: Full=Stress-inducible yeast MPV17 protein 1;
GN Name=SYM1; OrderedLocusNames=YLR251W;
OS Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast).
OC Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC Saccharomycetales; Saccharomycetaceae; Saccharomyces.
OX NCBI_TaxID=559292;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=9169871;
RA Johnston M., Hillier L.W., Riles L., Albermann K., Andre B., Ansorge W.,
RA Benes V., Brueckner M., Delius H., Dubois E., Duesterhoeft A.,
RA Entian K.-D., Floeth M., Goffeau A., Hebling U., Heumann K.,
RA Heuss-Neitzel D., Hilbert H., Hilger F., Kleine K., Koetter P., Louis E.J.,
RA Messenguy F., Mewes H.-W., Miosga T., Moestl D., Mueller-Auer S.,
RA Nentwich U., Obermaier B., Piravandi E., Pohl T.M., Portetelle D.,
RA Purnelle B., Rechmann S., Rieger M., Rinke M., Rose M., Scharfe M.,
RA Scherens B., Scholler P., Schwager C., Schwarz S., Underwood A.P.,
RA Urrestarazu L.A., Vandenbol M., Verhasselt P., Vierendeels F., Voet M.,
RA Volckaert G., Voss H., Wambutt R., Wedler E., Wedler H., Zimmermann F.K.,
RA Zollner A., Hani J., Hoheisel J.D.;
RT "The nucleotide sequence of Saccharomyces cerevisiae chromosome XII.";
RL Nature 387:87-90(1997).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=24374639; DOI=10.1534/g3.113.008995;
RA Engel S.R., Dietrich F.S., Fisk D.G., Binkley G., Balakrishnan R.,
RA Costanzo M.C., Dwight S.S., Hitz B.C., Karra K., Nash R.S., Weng S.,
RA Wong E.D., Lloyd P., Skrzypek M.S., Miyasato S.R., Simison M., Cherry J.M.;
RT "The reference genome sequence of Saccharomyces cerevisiae: Then and now.";
RL G3 (Bethesda) 4:389-398(2014).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ATCC 204508 / S288c;
RX PubMed=17322287; DOI=10.1101/gr.6037607;
RA Hu Y., Rolfs A., Bhullar B., Murthy T.V.S., Zhu C., Berger M.F.,
RA Camargo A.A., Kelley F., McCarron S., Jepson D., Richardson A., Raphael J.,
RA Moreira D., Taycher E., Zuo D., Mohr S., Kane M.F., Williamson J.,
RA Simpson A.J.G., Bulyk M.L., Harlow E., Marsischky G., Kolodner R.D.,
RA LaBaer J.;
RT "Approaching a complete repository of sequence-verified protein-encoding
RT clones for Saccharomyces cerevisiae.";
RL Genome Res. 17:536-543(2007).
RN [4]
RP LEVEL OF PROTEIN EXPRESSION [LARGE SCALE ANALYSIS].
RX PubMed=14562106; DOI=10.1038/nature02046;
RA Ghaemmaghami S., Huh W.-K., Bower K., Howson R.W., Belle A., Dephoure N.,
RA O'Shea E.K., Weissman J.S.;
RT "Global analysis of protein expression in yeast.";
RL Nature 425:737-741(2003).
RN [5]
RP FUNCTION, AND SUBCELLULAR LOCATION.
RX PubMed=15189984; DOI=10.1128/ec.3.3.620-631.2004;
RA Trott A., Morano K.A.;
RT "SYM1 is the stress-induced Saccharomyces cerevisiae ortholog of the
RT mammalian kidney disease gene Mpv17 and is required for ethanol metabolism
RT and tolerance during heat shock.";
RL Eukaryot. Cell 3:620-631(2004).
RN [6]
RP MUTAGENESIS OF ARG-54 AND ASN-172.
RX PubMed=16582910; DOI=10.1038/ng1765;
RA Spinazzola A., Viscomi C., Fernandez-Vizarra E., Carrara F., D'Adamo P.,
RA Calvo S., Marsano R.M., Donnini C., Weiher H., Strisciuglio P., Parini R.,
RA Sarzi E., Chan A., Dimauro S., Rotig A., Gasparini P., Ferrero I.,
RA Mootha V.K., Tiranti V., Zeviani M.;
RT "MPV17 encodes an inner mitochondrial membrane protein and is mutated in
RT infantile hepatic mitochondrial DNA depletion.";
RL Nat. Genet. 38:570-575(2006).
CC -!- FUNCTION: May be involved in cellular response to stress. Required to
CC maintain mitochondrial DNA (mtDNA) integrity and stability. Required
CC for ethanol metabolism and tolerance during heat shock.
CC {ECO:0000269|PubMed:15189984}.
CC -!- SUBCELLULAR LOCATION: Mitochondrion inner membrane
CC {ECO:0000269|PubMed:15189984}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:15189984}.
CC -!- MISCELLANEOUS: Present with 1870 molecules/cell in log phase SD medium.
CC {ECO:0000269|PubMed:14562106}.
CC -!- SIMILARITY: Belongs to the peroxisomal membrane protein PXMP2/4 family.
CC {ECO:0000305}.
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DR EMBL; U20865; AAB67389.1; -; Genomic_DNA.
DR EMBL; AY558211; AAS56537.1; -; Genomic_DNA.
DR EMBL; BK006945; DAA09565.1; -; Genomic_DNA.
DR PIR; S59397; S59397.
DR RefSeq; NP_013352.1; NM_001182138.1.
DR AlphaFoldDB; Q06563; -.
DR BioGRID; 31519; 57.
DR DIP; DIP-4568N; -.
DR IntAct; Q06563; 1.
DR STRING; 4932.YLR251W; -.
DR TCDB; 1.A.126.1.2; the mpv17/pmp22 4 tms putative channel (mpv17) family.
DR MaxQB; Q06563; -.
DR PaxDb; Q06563; -.
DR PRIDE; Q06563; -.
DR EnsemblFungi; YLR251W_mRNA; YLR251W; YLR251W.
DR GeneID; 850953; -.
DR KEGG; sce:YLR251W; -.
DR SGD; S000004241; SYM1.
DR VEuPathDB; FungiDB:YLR251W; -.
DR eggNOG; KOG1944; Eukaryota.
DR GeneTree; ENSGT00940000160891; -.
DR HOGENOM; CLU_049109_8_1_1; -.
DR InParanoid; Q06563; -.
DR OMA; KGWEDFE; -.
DR BioCyc; YEAST:G3O-32356-MON; -.
DR Reactome; R-SCE-9603798; Class I peroxisomal membrane protein import.
DR PRO; PR:Q06563; -.
DR Proteomes; UP000002311; Chromosome XII.
DR RNAct; Q06563; protein.
DR GO; GO:0005737; C:cytoplasm; IBA:GO_Central.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005743; C:mitochondrial inner membrane; IDA:SGD.
DR GO; GO:0005739; C:mitochondrion; HDA:SGD.
DR GO; GO:0006067; P:ethanol metabolic process; IMP:SGD.
DR InterPro; IPR007248; Mpv17_PMP22.
DR PANTHER; PTHR11266; PTHR11266; 1.
DR Pfam; PF04117; Mpv17_PMP22; 1.
PE 1: Evidence at protein level;
KW Membrane; Mitochondrion; Mitochondrion inner membrane; Reference proteome;
KW Transmembrane; Transmembrane helix.
FT CHAIN 1..197
FT /note="Protein SYM1"
FT /id="PRO_0000234417"
FT TRANSMEM 20..40
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 55..75
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 97..117
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 137..157
FT /note="Helical"
FT /evidence="ECO:0000255"
FT MUTAGEN 54
FT /note="R->Q: Almost abolishes function."
FT /evidence="ECO:0000269|PubMed:16582910"
FT MUTAGEN 54
FT /note="R->W: Loss of function."
FT /evidence="ECO:0000269|PubMed:16582910"
FT MUTAGEN 172
FT /note="N->K: Loss of function."
FT /evidence="ECO:0000269|PubMed:16582910"
SQ SEQUENCE 197 AA; 22915 MW; 0F3FFBA63C557547 CRC64;
MKLLHLYEAS LKRRPKTTNA IMTGALFGIG DVSAQLLFPT SKVNKGYDYK RTARAVIYGS
LIFSFIGDKW YKILNNKIYM RNRPQYHWSN MVLRVAVDQL AFAPLGLPFY FTCMSIMEGR
SFDVAKLKIK EQWWPTLLTN WAVWPLFQAI NFSVVPLQHR LLAVNVVAIF WNTYLSYKNS
KVMEKDKVPV HYPPVVE
