ID   SYMC_NOSCE              Reviewed;         560 AA.
AC   C4V943;
DT   03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   03-NOV-2009, sequence version 2.
DT   03-AUG-2022, entry version 59.
DE   RecName: Full=Probable methionine--tRNA ligase, cytoplasmic;
DE            EC=6.1.1.10;
DE   AltName: Full=Methionyl-tRNA synthetase;
DE            Short=MetRS;
GN   ORFNames=NCER_101052;
OS   Nosema ceranae (strain BRL01) (Microsporidian parasite).
OC   Eukaryota; Fungi; Fungi incertae sedis; Microsporidia; Nosematidae; Nosema.
OX   NCBI_TaxID=578460;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=BRL01;
RX   PubMed=19503607; DOI=10.1371/journal.ppat.1000466;
RA   Cornman R.S., Chen Y.P., Schatz M.C., Street C., Zhao Y., Desany B.,
RA   Egholm M., Hutchison S., Pettis J.S., Lipkin W.I., Evans J.D.;
RT   "Genomic analyses of the microsporidian Nosema ceranae, an emergent
RT   pathogen of honey bees.";
RL   PLoS Pathog. 5:E1000466-E1000466(2009).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-
CC         methionyl-tRNA(Met); Xref=Rhea:RHEA:13481, Rhea:RHEA-COMP:9667,
CC         Rhea:RHEA-COMP:9698, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:57844, ChEBI:CHEBI:78442, ChEBI:CHEBI:78530,
CC         ChEBI:CHEBI:456215; EC=6.1.1.10;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=EEQ82257.1; Type=Frameshift; Evidence={ECO:0000305};
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DR   EMBL; ACOL01000084; EEQ82257.1; ALT_FRAME; Genomic_DNA.
DR   RefSeq; XP_002995928.1; XM_002995882.1.
DR   AlphaFoldDB; C4V943; -.
DR   SMR; C4V943; -.
DR   STRING; 578460.C4V943; -.
DR   EnsemblFungi; EEQ82257; EEQ82257; NCER_101052.
DR   KEGG; nce:NCER_101052; -.
DR   HOGENOM; CLU_009710_1_2_1; -.
DR   InParanoid; C4V943; -.
DR   Proteomes; UP000009082; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004825; F:methionine-tRNA ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006431; P:methionyl-tRNA aminoacylation; IEA:InterPro.
DR   CDD; cd00814; MetRS_core; 1.
DR   Gene3D; 2.20.28.20; -; 1.
DR   Gene3D; 3.40.50.620; -; 1.
DR   InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR   InterPro; IPR041872; Anticodon_Met.
DR   InterPro; IPR023458; Met-tRNA_ligase_1.
DR   InterPro; IPR014758; Met-tRNA_synth.
DR   InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR   InterPro; IPR033911; MetRS_core.
DR   InterPro; IPR029038; MetRS_Zn.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   PANTHER; PTHR45765; PTHR45765; 1.
DR   Pfam; PF19303; Anticodon_3; 1.
DR   Pfam; PF09334; tRNA-synt_1g; 1.
DR   PRINTS; PR01041; TRNASYNTHMET.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF57770; SSF57770; 1.
DR   TIGRFAMs; TIGR00398; metG; 1.
DR   PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..560
FT                   /note="Probable methionine--tRNA ligase, cytoplasmic"
FT                   /id="PRO_0000388398"
FT   MOTIF           16..26
FT                   /note="'HIGH' region"
FT                   /evidence="ECO:0000250"
FT   MOTIF           347..351
FT                   /note="'KMSKS' region"
FT                   /evidence="ECO:0000250"
FT   BINDING         350
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   560 AA;  65264 MW;  A52A29503EC6867A CRC64;
     MKKKFLVKKI ITSALPYVNN QPHLGNIIGC VLSADMYARF CRKNNEDVVF LSGTDEYGTA
     IEMAAFAQNK TPLQICEENR IIHKKIYDWF NIDFDFFGHT TSTAHTKNVQ DFFNKIHNNG
     FFTEQEIEQF FCDKCQIFLA DRYVVGTCKF CKYTDAKGDQ CDGCGHTYKS LDLIDAKCTL
     CHSFPNIKST RHLFFDFNNF KDKLQKLFNT NSQYWSENGK QITKSWLDQE LLPRCMTRDL
     KNRWGVPVPL KDFEEKVFYV WFDAVIGYFT FYKEYVAARS ESKELDKNNV FATDNILQDC
     ELVQFMGKDN VFFHTIVFPS LIFATNDSYP LIKKLSVTEF LLFENEKFSK SRGHGIFGLD
     LVDNTMGQSC LWRYYLAKIR PEKCDSNFSF THFTNVIDAD LNNNIGNFCN RVLKYIKNKN
     DKLISIDKLE HRDDLMVQTI DKIYKEYLTS FSAIRIREAL EKILEISKVG NEYVQQVVSS
     KIEVPKGFQV AFSIVVLIGQ LLEPFIPVSS EKLLKMCNRK KENFCESFYI IKSAEIGDDI
     KPLFNKLDDS LIERIKNFKK