SYME_ECOLI
ID SYME_ECOLI Reviewed; 113 AA.
AC P39394; Q2M5W9;
DT 01-FEB-1995, integrated into UniProtKB/Swiss-Prot.
DT 10-JUL-2007, sequence version 2.
DT 03-AUG-2022, entry version 131.
DE RecName: Full=Toxic protein SymE;
DE AltName: Full=Putative endoribonuclease SymE;
DE EC=3.1.-.-;
GN Name=symE; Synonyms=dinL, sosC, yjiW; OrderedLocusNames=b4347, JW4310;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=7610040; DOI=10.1093/nar/23.12.2105;
RA Burland V.D., Plunkett G. III, Sofia H.J., Daniels D.L., Blattner F.R.;
RT "Analysis of the Escherichia coli genome VI: DNA sequence of the region
RT from 92.8 through 100 minutes.";
RL Nucleic Acids Res. 23:2105-2119(1995).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP REGULATION BY LEXA, AND INDUCTION.
RC STRAIN=K12 / RW118;
RX PubMed=10760155; DOI=10.1046/j.1365-2958.2000.01826.x;
RA Fernandez De Henestrosa A.R., Ogi T., Aoyagi S., Chafin D., Hayes J.J.,
RA Ohmori H., Woodgate R.;
RT "Identification of additional genes belonging to the LexA regulon in
RT Escherichia coli.";
RL Mol. Microbiol. 35:1560-1572(2000).
RN [5]
RP FUNCTION, AND INDUCTION.
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=17462020; DOI=10.1111/j.1365-2958.2007.05688.x;
RA Kawano M., Aravind L., Storz G.;
RT "An antisense RNA controls synthesis of an SOS-induced toxin evolved from
RT an antitoxin.";
RL Mol. Microbiol. 64:738-754(2007).
CC -!- FUNCTION: Toxic component of a type I toxin-antitoxin (TA) system
CC (PubMed:17462020). Involved in the degradation and recycling of damaged
CC RNA (PubMed:17462020). It is itself a target for degradation by the
CC ATP-dependent protease Lon (PubMed:17462020).
CC {ECO:0000269|PubMed:17462020}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000305|PubMed:17462020}.
CC Note=Purifies with ribosomes. {ECO:0000269|PubMed:17462020}.
CC -!- INDUCTION: Repressed by LexA, induced by DNA damage (PubMed:10760155,
CC PubMed:17462020). During the SOS response (at protein level when
CC expressed as a fusion protein) (PubMed:17462020). The mRNA stability
CC and translation is controlled by the small regulatory RNA symR, which
CC probably blocks ribosome binding (PubMed:17462020). Expression of the
CC proteinaceous toxin (putative endonuclease) is controlled by antisense
CC sRNA SymR (PubMed:17462020). {ECO:0000269|PubMed:10760155,
CC ECO:0000269|PubMed:17462020}.
CC -!- MISCELLANEOUS: Overexpression of this protein is toxic for the cell,
CC affecting colony-forming ability and protein synthesis.
CC {ECO:0000269|PubMed:17462020}.
CC -!- SIMILARITY: Belongs to the SymE family. {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=AAA97244.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
CC Sequence=BAE78337.1; Type=Erroneous initiation; Note=Extended N-terminus.; Evidence={ECO:0000305};
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DR EMBL; U14003; AAA97244.1; ALT_INIT; Genomic_DNA.
DR EMBL; U00096; AAC77303.2; -; Genomic_DNA.
DR EMBL; AP009048; BAE78337.1; ALT_INIT; Genomic_DNA.
DR PIR; S56573; S56573.
DR RefSeq; NP_418767.4; NC_000913.3.
DR RefSeq; WP_000132601.1; NZ_LN832404.1.
DR AlphaFoldDB; P39394; -.
DR BioGRID; 4262766; 164.
DR STRING; 511145.b4347; -.
DR PaxDb; P39394; -.
DR PRIDE; P39394; -.
DR EnsemblBacteria; AAC77303; AAC77303; b4347.
DR EnsemblBacteria; BAE78337; BAE78337; BAE78337.
DR GeneID; 949088; -.
DR KEGG; ecj:JW4310; -.
DR KEGG; eco:b4347; -.
DR PATRIC; fig|1411691.4.peg.2339; -.
DR EchoBASE; EB2470; -.
DR eggNOG; ENOG5031VID; Bacteria.
DR HOGENOM; CLU_151239_0_0_6; -.
DR OMA; MRQHTRT; -.
DR PhylomeDB; P39394; -.
DR BioCyc; EcoCyc:G7940-MON; -.
DR PRO; PR:P39394; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0004521; F:endoribonuclease activity; IEA:UniProtKB-UniRule.
DR GO; GO:0003723; F:RNA binding; IEA:UniProtKB-KW.
DR GO; GO:0006974; P:cellular response to DNA damage stimulus; IEP:EcoliWiki.
DR GO; GO:0006402; P:mRNA catabolic process; IMP:EcoCyc.
DR GO; GO:0009432; P:SOS response; IEP:EcoCyc.
DR HAMAP; MF_01193; Endoribonucl_SymE; 1.
DR InterPro; IPR007159; SpoVT-AbrB_dom.
DR InterPro; IPR014944; Toxin_SymE-like.
DR InterPro; IPR020883; TypeI_TA_SymE.
DR Pfam; PF08845; SymE_toxin; 1.
DR PROSITE; PS51740; SPOVT_ABRB; 1.
PE 2: Evidence at transcript level;
KW Cytoplasm; DNA-binding; Endonuclease; Hydrolase; Nuclease;
KW Reference proteome; RNA-binding; Toxin-antitoxin system.
FT CHAIN 1..113
FT /note="Toxic protein SymE"
FT /id="PRO_0000169799"
FT DOMAIN 29..74
FT /note="SpoVT-AbrB"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU01076"
SQ SEQUENCE 113 AA; 12203 MW; 4C3C0CE696CE7D9D CRC64;
MTDTHSIAQP FEAEVSPANN RHVTVGYASR YPDYSRIPAI TLKGQWLEAA GFATGTAVDV
KVMEGCIVLT AQPPAAEESE LMQSLRQVCK LSARKQKQVQ AFIGVIAGKQ KVA
