BLUR_ECOLI
ID BLUR_ECOLI Reviewed; 243 AA.
AC P75989; Q9R7N5;
DT 15-JUL-1998, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1997, sequence version 1.
DT 03-AUG-2022, entry version 163.
DE RecName: Full=HTH-type transcriptional repressor BluR {ECO:0000305};
GN Name=bluR {ECO:0000303|PubMed:22783906}; Synonyms=ycgE;
GN OrderedLocusNames=b1162, JW1149;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=8905232; DOI=10.1093/dnares/3.3.137;
RA Oshima T., Aiba H., Baba T., Fujita K., Hayashi K., Honjo A., Ikemoto K.,
RA Inada T., Itoh T., Kajihara M., Kanai K., Kashimoto K., Kimura S.,
RA Kitagawa M., Makino K., Masuda S., Miki T., Mizobuchi K., Mori H.,
RA Motomura K., Nakamura Y., Nashimoto H., Nishio Y., Saito N., Sampei G.,
RA Seki Y., Tagami H., Takemoto K., Wada C., Yamamoto Y., Yano M.,
RA Horiuchi T.;
RT "A 718-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 12.7-28.0 min region on the linkage map.";
RL DNA Res. 3:137-155(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [4]
RP ROLE IN REGULATION OF OMPF IN ASSOCIATION WITH LON, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=K12 / AG100;
RX PubMed=19721064; DOI=10.1128/aac.00787-09;
RA Duval V., Nicoloff H., Levy S.B.;
RT "Combined inactivation of lon and ycgE decreases multidrug susceptibility
RT by reducing the amount of OmpF porin in Escherichia coli.";
RL Antimicrob. Agents Chemother. 53:4944-4948(2009).
RN [5]
RP FUNCTION, INTERACTION WITH BLUF, DNA-BINDING, INDUCTION, AND DISRUPTION
RP PHENOTYPE.
RC STRAIN=K12 / MC4100;
RX PubMed=19240136; DOI=10.1101/gad.499409;
RA Tschowri N., Busse S., Hengge R.;
RT "The BLUF-EAL protein YcgF acts as a direct anti-repressor in a blue-light
RT response of Escherichia coli.";
RL Genes Dev. 23:522-534(2009).
RN [6]
RP FUNCTION, DNA-BINDING, AND INTERACTION WITH BLUF.
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=22783906; DOI=10.1111/j.1365-2958.2012.08147.x;
RA Tschowri N., Lindenberg S., Hengge R.;
RT "Molecular function and potential evolution of the biofilm-modulating blue
RT light-signalling pathway of Escherichia coli.";
RL Mol. Microbiol. 85:893-906(2012).
CC -!- FUNCTION: Controls the expression of several small proteins that may
CC play a role in biofilm maturation. Binds to and represses the operator
CC of the ycgZ-ymgA-ariR-ymgC operon and also regulates ynaK. Binding is
CC antagonized by BluF upon blue light (470 nm) irradiation. Blue light
CC may increase the affinity of BluF for BluR, allowing it to be released
CC from its operator. {ECO:0000269|PubMed:19240136,
CC ECO:0000269|PubMed:22783906}.
CC -!- SUBUNIT: Interacts with BluF. {ECO:0000269|PubMed:19240136,
CC ECO:0000269|PubMed:22783906}.
CC -!- INTERACTION:
CC P75989; P23874: hipA; NbExp=2; IntAct=EBI-560582, EBI-560590;
CC P75989; P0A9P0: lpdA; NbExp=2; IntAct=EBI-560582, EBI-542856;
CC -!- INDUCTION: Expression is strongly activated (7-fold at 16 degrees
CC Celsius compared to 37 degrees) at low temperature.
CC {ECO:0000269|PubMed:19240136}.
CC -!- DISRUPTION PHENOTYPE: 3- to 20-fold derepression of several genes
CC coding for small proteins including the ycgZ-ymgA-ariR-ymgC operon.
CC While the BluR/F system is induced at low temperatures under blue light
CC irradiation, it is not essential for growth under these conditions.
CC When both lon and bluR are disrupted, levels of OmpF decrease, leading
CC to lower drug susceptibility, with a greater effect at 26 degrees than
CC at 37 degrees Celsius. The mechanism is not yet understood.
CC {ECO:0000269|PubMed:19240136, ECO:0000269|PubMed:19721064}.
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DR EMBL; U00096; AAC74246.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA35997.1; -; Genomic_DNA.
DR PIR; G64861; G64861.
DR RefSeq; NP_415680.1; NC_000913.3.
DR RefSeq; WP_000332303.1; NZ_STEB01000023.1.
DR AlphaFoldDB; P75989; -.
DR SMR; P75989; -.
DR BioGRID; 4260084; 6.
DR DIP; DIP-11552N; -.
DR IntAct; P75989; 39.
DR STRING; 511145.b1162; -.
DR jPOST; P75989; -.
DR PaxDb; P75989; -.
DR PRIDE; P75989; -.
DR EnsemblBacteria; AAC74246; AAC74246; b1162.
DR EnsemblBacteria; BAA35997; BAA35997; BAA35997.
DR GeneID; 66675022; -.
DR GeneID; 945735; -.
DR KEGG; ecj:JW1149; -.
DR KEGG; eco:b1162; -.
DR PATRIC; fig|1411691.4.peg.1128; -.
DR EchoBASE; EB3645; -.
DR eggNOG; COG0789; Bacteria.
DR HOGENOM; CLU_045945_0_1_6; -.
DR InParanoid; P75989; -.
DR OMA; CPVEVFD; -.
DR PhylomeDB; P75989; -.
DR BioCyc; EcoCyc:G6602-MON; -.
DR PRO; PR:P75989; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR GO; GO:0003700; F:DNA-binding transcription factor activity; IDA:EcoCyc.
DR GO; GO:0045892; P:negative regulation of transcription, DNA-templated; IDA:EcoCyc.
DR InterPro; IPR009061; DNA-bd_dom_put_sf.
DR InterPro; IPR000551; MerR-type_HTH_dom.
DR Pfam; PF13411; MerR_1; 1.
DR SMART; SM00422; HTH_MERR; 1.
DR SUPFAM; SSF46955; SSF46955; 1.
DR PROSITE; PS00552; HTH_MERR_1; 1.
DR PROSITE; PS50937; HTH_MERR_2; 1.
PE 1: Evidence at protein level;
KW DNA-binding; Reference proteome; Repressor; Transcription;
KW Transcription regulation.
FT CHAIN 1..243
FT /note="HTH-type transcriptional repressor BluR"
FT /id="PRO_0000098165"
FT DOMAIN 3..72
FT /note="HTH merR-type"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00254"
FT DNA_BIND 6..25
FT /note="H-T-H motif"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00254"
SQ SEQUENCE 243 AA; 28265 MW; E8E5AD376A18EB84 CRC64;
MAYYSIGDVA ERCGINPVTL RAWQRRYGLL KPQRSEGGHR LFDEEDIQRI EEIKRWISNG
VPVGKVKALL ETTSQDTEDD WSRLQEEMMS ILRMANPAKL RARIISLGRE YPVDQLINHV
YLPVRQRLVL DHNTSRIMSS MFDGALIEYA ATSLFEMRRK PGKEAILMAW NVEERARLWL
EAWRLSLSGW HISVLADPIE SPRPELFPTQ TLIVWTGMAP TRRQNELLQH WGEQGYKVIF
HAP
