SYMM_ARATH
ID SYMM_ARATH Reviewed; 616 AA.
AC Q9M2T9; O23761; Q9ASP8;
DT 22-JUL-2015, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 146.
DE RecName: Full=Methionine--tRNA ligase, chloroplastic/mitochondrial {ECO:0000305};
DE EC=6.1.1.10 {ECO:0000305};
DE AltName: Full=Methionyl-tRNA synthetase {ECO:0000305};
DE Short=AtcpMetRS {ECO:0000303|PubMed:9724821};
DE Short=MetRS {ECO:0000305};
DE AltName: Full=Protein OVULE ABORTION 1 {ECO:0000303|PubMed:16297076};
DE Flags: Precursor;
GN Name=OVA1 {ECO:0000303|PubMed:16297076};
GN OrderedLocusNames=At3g55400 {ECO:0000312|Araport:AT3G55400};
GN ORFNames=T22E16.60 {ECO:0000312|EMBL:CAB75898.1};
OS Arabidopsis thaliana (Mouse-ear cress).
OC Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX NCBI_TaxID=3702;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND SUBCELLULAR LOCATION.
RC STRAIN=cv. Columbia;
RX PubMed=9724821; DOI=10.1073/pnas.95.18.11014;
RA Menand B., Marechal-Drouard L., Sakamoto W., Dietrich A., Wintz H.;
RT "A single gene of chloroplast origin codes for mitochondrial and
RT chloroplastic methionyl-tRNA synthetase in Arabidopsis thaliana.";
RL Proc. Natl. Acad. Sci. U.S.A. 95:11014-11019(1998).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=cv. Columbia;
RX PubMed=11130713; DOI=10.1038/35048706;
RA Salanoubat M., Lemcke K., Rieger M., Ansorge W., Unseld M., Fartmann B.,
RA Valle G., Bloecker H., Perez-Alonso M., Obermaier B., Delseny M.,
RA Boutry M., Grivell L.A., Mache R., Puigdomenech P., De Simone V.,
RA Choisne N., Artiguenave F., Robert C., Brottier P., Wincker P.,
RA Cattolico L., Weissenbach J., Saurin W., Quetier F., Schaefer M.,
RA Mueller-Auer S., Gabel C., Fuchs M., Benes V., Wurmbach E., Drzonek H.,
RA Erfle H., Jordan N., Bangert S., Wiedelmann R., Kranz H., Voss H.,
RA Holland R., Brandt P., Nyakatura G., Vezzi A., D'Angelo M., Pallavicini A.,
RA Toppo S., Simionati B., Conrad A., Hornischer K., Kauer G., Loehnert T.-H.,
RA Nordsiek G., Reichelt J., Scharfe M., Schoen O., Bargues M., Terol J.,
RA Climent J., Navarro P., Collado C., Perez-Perez A., Ottenwaelder B.,
RA Duchemin D., Cooke R., Laudie M., Berger-Llauro C., Purnelle B., Masuy D.,
RA de Haan M., Maarse A.C., Alcaraz J.-P., Cottet A., Casacuberta E.,
RA Monfort A., Argiriou A., Flores M., Liguori R., Vitale D., Mannhaupt G.,
RA Haase D., Schoof H., Rudd S., Zaccaria P., Mewes H.-W., Mayer K.F.X.,
RA Kaul S., Town C.D., Koo H.L., Tallon L.J., Jenkins J., Rooney T., Rizzo M.,
RA Walts A., Utterback T., Fujii C.Y., Shea T.P., Creasy T.H., Haas B.,
RA Maiti R., Wu D., Peterson J., Van Aken S., Pai G., Militscher J.,
RA Sellers P., Gill J.E., Feldblyum T.V., Preuss D., Lin X., Nierman W.C.,
RA Salzberg S.L., White O., Venter J.C., Fraser C.M., Kaneko T., Nakamura Y.,
RA Sato S., Kato T., Asamizu E., Sasamoto S., Kimura T., Idesawa K.,
RA Kawashima K., Kishida Y., Kiyokawa C., Kohara M., Matsumoto M., Matsuno A.,
RA Muraki A., Nakayama S., Nakazaki N., Shinpo S., Takeuchi C., Wada T.,
RA Watanabe A., Yamada M., Yasuda M., Tabata S.;
RT "Sequence and analysis of chromosome 3 of the plant Arabidopsis thaliana.";
RL Nature 408:820-822(2000).
RN [3]
RP GENOME REANNOTATION.
RC STRAIN=cv. Columbia;
RX PubMed=27862469; DOI=10.1111/tpj.13415;
RA Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA Town C.D.;
RT "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT genome.";
RL Plant J. 89:789-804(2017).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=cv. Columbia;
RX PubMed=14593172; DOI=10.1126/science.1088305;
RA Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA Ecker J.R.;
RT "Empirical analysis of transcriptional activity in the Arabidopsis
RT genome.";
RL Science 302:842-846(2003).
RN [5]
RP DISRUPTION PHENOTYPE.
RX PubMed=16297076; DOI=10.1111/j.1365-313x.2005.02580.x;
RA Berg M., Rogers R., Muralla R., Meinke D.;
RT "Requirement of aminoacyl-tRNA synthetases for gametogenesis and embryo
RT development in Arabidopsis.";
RL Plant J. 44:866-878(2005).
RN [6]
RP SUBCELLULAR LOCATION.
RX PubMed=17433818; DOI=10.1016/j.jmb.2007.03.015;
RA Pujol C., Marechal-Drouard L., Duchene A.M.;
RT "How can organellar protein N-terminal sequences be dual targeting signals?
RT In silico analysis and mutagenesis approach.";
RL J. Mol. Biol. 369:356-367(2007).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-
CC methionyl-tRNA(Met); Xref=Rhea:RHEA:13481, Rhea:RHEA-COMP:9667,
CC Rhea:RHEA-COMP:9698, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:57844, ChEBI:CHEBI:78442, ChEBI:CHEBI:78530,
CC ChEBI:CHEBI:456215; EC=6.1.1.10; Evidence={ECO:0000305};
CC -!- SUBCELLULAR LOCATION: Plastid, chloroplast
CC {ECO:0000269|PubMed:17433818, ECO:0000269|PubMed:9724821}.
CC Mitochondrion {ECO:0000269|PubMed:17433818,
CC ECO:0000269|PubMed:9724821}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=1;
CC Comment=A number of isoforms are produced. According EST sequences.
CC {ECO:0000305};
CC Name=1;
CC IsoId=Q9M2T9-1; Sequence=Displayed;
CC -!- DISRUPTION PHENOTYPE: Lethal. In heterozygous plants, aborted ovules.
CC {ECO:0000269|PubMed:16297076}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000305}.
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DR EMBL; Y13943; CAA74281.1; -; mRNA.
DR EMBL; AL132975; CAB75898.1; -; Genomic_DNA.
DR EMBL; CP002686; AEE79379.1; -; Genomic_DNA.
DR EMBL; AF367354; AAK32940.1; -; mRNA.
DR EMBL; AY133597; AAM91427.1; -; mRNA.
DR PIR; T47679; T47679.
DR PIR; T50641; T50641.
DR RefSeq; NP_191100.1; NM_115398.3. [Q9M2T9-1]
DR AlphaFoldDB; Q9M2T9; -.
DR SMR; Q9M2T9; -.
DR STRING; 3702.AT3G55400.1; -.
DR PaxDb; Q9M2T9; -.
DR PRIDE; Q9M2T9; -.
DR ProteomicsDB; 234126; -. [Q9M2T9-1]
DR EnsemblPlants; AT3G55400.1; AT3G55400.1; AT3G55400. [Q9M2T9-1]
DR GeneID; 824706; -.
DR Gramene; AT3G55400.1; AT3G55400.1; AT3G55400. [Q9M2T9-1]
DR KEGG; ath:AT3G55400; -.
DR Araport; AT3G55400; -.
DR TAIR; locus:2099966; AT3G55400.
DR eggNOG; KOG0436; Eukaryota.
DR InParanoid; Q9M2T9; -.
DR OMA; SDMHGTP; -.
DR OrthoDB; 788159at2759; -.
DR PhylomeDB; Q9M2T9; -.
DR BRENDA; 6.1.1.10; 399.
DR PRO; PR:Q9M2T9; -.
DR Proteomes; UP000006548; Chromosome 3.
DR ExpressionAtlas; Q9M2T9; baseline and differential.
DR Genevisible; Q9M2T9; AT.
DR GO; GO:0009507; C:chloroplast; IDA:TAIR.
DR GO; GO:0009570; C:chloroplast stroma; HDA:TAIR.
DR GO; GO:0005739; C:mitochondrion; IDA:TAIR.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004825; F:methionine-tRNA ligase activity; IDA:TAIR.
DR GO; GO:0006431; P:methionyl-tRNA aminoacylation; IBA:GO_Central.
DR GO; GO:0048481; P:plant ovule development; IMP:TAIR.
DR CDD; cd07957; Anticodon_Ia_Met; 1.
DR CDD; cd00814; MetRS_core; 1.
DR Gene3D; 3.40.50.620; -; 1.
DR HAMAP; MF_01228; Met_tRNA_synth_type2; 1.
DR InterPro; IPR002300; aa-tRNA-synth_Ia.
DR InterPro; IPR041872; Anticodon_Met.
DR InterPro; IPR014758; Met-tRNA_synth.
DR InterPro; IPR023457; Met-tRNA_synth_2.
DR InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR InterPro; IPR033911; MetRS_core.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR PANTHER; PTHR43326; PTHR43326; 1.
DR Pfam; PF19303; Anticodon_3; 1.
DR Pfam; PF00133; tRNA-synt_1; 1.
DR Pfam; PF09334; tRNA-synt_1g; 1.
DR PRINTS; PR01041; TRNASYNTHMET.
DR SUPFAM; SSF47323; SSF47323; 1.
DR TIGRFAMs; TIGR00398; metG; 1.
PE 2: Evidence at transcript level;
KW Alternative splicing; Aminoacyl-tRNA synthetase; ATP-binding; Chloroplast;
KW Ligase; Mitochondrion; Nucleotide-binding; Plastid; Protein biosynthesis;
KW Reference proteome; Transit peptide.
FT TRANSIT 1..?
FT /note="Chloroplast and mitochondrion"
FT /evidence="ECO:0000305"
FT CHAIN ?..616
FT /note="Methionine--tRNA ligase,
FT chloroplastic/mitochondrial"
FT /evidence="ECO:0000305"
FT /id="PRO_0000433535"
FT REGION 582..602
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOTIF 78..88
FT /note="'HIGH' region"
FT /evidence="ECO:0000305"
FT MOTIF 366..370
FT /note="'KMSKS' region"
FT /evidence="ECO:0000305"
FT BINDING 369
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT CONFLICT 99
FT /note="A -> S (in Ref. 1; CAA74281)"
FT /evidence="ECO:0000305"
FT CONFLICT 204
FT /note="E -> G (in Ref. 4; AAK32940/AAM91427)"
FT /evidence="ECO:0000305"
FT CONFLICT 344
FT /note="M -> I (in Ref. 1; CAA74281)"
FT /evidence="ECO:0000305"
FT CONFLICT 540
FT /note="I -> V (in Ref. 1; CAA74281)"
FT /evidence="ECO:0000305"
FT CONFLICT 547
FT /note="T -> S (in Ref. 1; CAA74281)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 616 AA; 69274 MW; 1E6A0B4DE127B4DC CRC64;
MAARINTSLH NALSFLKPFN TPLNTKPFSF RRNSFRFSKK LPYYSQFSSG KRALYCTSSS
QESTVDEGET FVLTTPLYYV NAPPHMGSAY TTIAADSIAR FQRLLGKKVI FITGTDEHGE
KIATSAAANG RNPPEHCDLI SQSYRTLWKD LDIAYDKFIR TTDPKHEAIV KEFYARVFAN
GDIYRADYEG LYCVNCEEYK DEKELLENNC CPVHQMPCVA RKEDNYFFAL SKYQKPLEDI
LAQNPRFVQP SYRLNEVQSW IKSGLRDFSI SRALVDWGIP VPDDDKQTIY VWFDALLGYI
SALTEDNKQQ NLETAVSFGW PASLHLIGKD ILRFHAVYWP AMLMSAGLEL PKMVFGHGFL
TKDGMKMGKS LGNTLEPFEL VQKFGPDAVR YFFLREVEFG NDGDYSEDRF IKIVNAHLAN
TIGNLLNRTL GLLKKNCEST LVVDSTVAAE GVPLKDTVEK LVEKARTNYE NLSLSSACEA
VLEIGNAGNT YMDQRAPWFL FKQGGVSAEE AAKDLVIILE VMRVIAVALS PVAPCLSLRI
YSQLGYTEDQ FNSITWSDTK WGGLKGGQVM EQASPVFARI ELNPEKEEDE KKPKVGKKTG
KAKVKVVEQT PTVAEA
