ID   SYMM_BOVIN              Reviewed;         593 AA.
AC   A6H7E1; Q0V8Q7;
DT   08-APR-2008, integrated into UniProtKB/Swiss-Prot.
DT   24-JUL-2007, sequence version 1.
DT   03-AUG-2022, entry version 96.
DE   RecName: Full=Methionine--tRNA ligase, mitochondrial;
DE            EC=6.1.1.10;
DE   AltName: Full=Methionyl-tRNA synthetase 2;
DE   AltName: Full=Mitochondrial methionyl-tRNA synthetase;
DE            Short=MtMetRS;
DE   Flags: Precursor;
GN   Name=MARS2;
OS   Bos taurus (Bovine).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Laurasiatheria; Artiodactyla; Ruminantia; Pecora; Bovidae;
OC   Bovinae; Bos.
OX   NCBI_TaxID=9913;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=Hereford; TISSUE=Fetal skin;
RG   NIH - Mammalian Gene Collection (MGC) project;
RL   Submitted (JUN-2007) to the EMBL/GenBank/DDBJ databases.
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 5-593.
RX   PubMed=16305752; DOI=10.1186/1471-2164-6-166;
RA   Harhay G.P., Sonstegard T.S., Keele J.W., Heaton M.P., Clawson M.L.,
RA   Snelling W.M., Wiedmann R.T., Van Tassell C.P., Smith T.P.L.;
RT   "Characterization of 954 bovine full-CDS cDNA sequences.";
RL   BMC Genomics 6:166-166(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-
CC         methionyl-tRNA(Met); Xref=Rhea:RHEA:13481, Rhea:RHEA-COMP:9667,
CC         Rhea:RHEA-COMP:9698, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:57844, ChEBI:CHEBI:78442, ChEBI:CHEBI:78530,
CC         ChEBI:CHEBI:456215; EC=6.1.1.10;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000305}.
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DR   EMBL; BC146211; AAI46212.1; -; mRNA.
DR   EMBL; BT026161; ABG67000.1; -; mRNA.
DR   RefSeq; NP_001092441.1; NM_001098971.1.
DR   AlphaFoldDB; A6H7E1; -.
DR   SMR; A6H7E1; -.
DR   STRING; 9913.ENSBTAP00000042279; -.
DR   PaxDb; A6H7E1; -.
DR   PRIDE; A6H7E1; -.
DR   Ensembl; ENSBTAT00000002662; ENSBTAP00000042279; ENSBTAG00000002056.
DR   GeneID; 514726; -.
DR   KEGG; bta:514726; -.
DR   CTD; 92935; -.
DR   VEuPathDB; HostDB:ENSBTAG00000002056; -.
DR   VGNC; VGNC:31251; MARS2.
DR   eggNOG; KOG0436; Eukaryota.
DR   GeneTree; ENSGT00550000075136; -.
DR   HOGENOM; CLU_009710_9_0_1; -.
DR   InParanoid; A6H7E1; -.
DR   OMA; SDMHGTP; -.
DR   OrthoDB; 788159at2759; -.
DR   TreeFam; TF105709; -.
DR   Proteomes; UP000009136; Chromosome 2.
DR   Bgee; ENSBTAG00000002056; Expressed in placenta and 103 other tissues.
DR   GO; GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004825; F:methionine-tRNA ligase activity; IBA:GO_Central.
DR   GO; GO:0006431; P:methionyl-tRNA aminoacylation; IBA:GO_Central.
DR   CDD; cd07957; Anticodon_Ia_Met; 1.
DR   CDD; cd00814; MetRS_core; 1.
DR   Gene3D; 3.40.50.620; -; 1.
DR   InterPro; IPR041872; Anticodon_Met.
DR   InterPro; IPR014758; Met-tRNA_synth.
DR   InterPro; IPR023457; Met-tRNA_synth_2.
DR   InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR   InterPro; IPR033911; MetRS_core.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   PANTHER; PTHR43326; PTHR43326; 1.
DR   Pfam; PF19303; Anticodon_3; 1.
DR   Pfam; PF09334; tRNA-synt_1g; 1.
DR   PRINTS; PR01041; TRNASYNTHMET.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   TIGRFAMs; TIGR00398; metG; 1.
PE   2: Evidence at transcript level;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Ligase; Mitochondrion;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome;
KW   Transit peptide.
FT   TRANSIT         1..29
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           30..593
FT                   /note="Methionine--tRNA ligase, mitochondrial"
FT                   /id="PRO_0000328569"
FT   MOTIF           52..62
FT                   /note="'HIGH' region"
FT   MOTIF           347..351
FT                   /note="'KMSKS' region"
FT   BINDING         350
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   593 AA;  66648 MW;  1B2593DC8AA40F1C CRC64;
     MLRVSAFRLL GRRGASRVSL LEDFSFRYYS SGPLGVRDDT RDSRAYFTTP IFYVNAAPHI
     GHLYSALLAD ALCRHHRLRV PSDAATGFST GTDEHGLKIQ QAAAAAGLAP SELCDRVSAQ
     FQQLFREADI SSTDFIRTTE ARHRIAVQHF WGMLKSRGLL YKGLYEGWYC ASDECFLPEA
     KVTRQPGPSG DLCPVSLESG HPVSWTKEEN YIFRLSQFRE PLQQWLRGDP QAITPEPFHH
     TVLQWLEEEL PDLSVSRRSS HLHWGIPVPG DDSQTIYVWL DALVNYLTVV GYPDAEFKSW
     WPNTSHIIGK DILKFHAIYW PALLLGAGMS PPHRIYVHSH WTVCGQKMSK SLGNVVDPRT
     CLDRYTVDGF RYFLLRQGVP SWDCDYYDEK VVKLLDSELA DALGGLLNRC TANKINPSGI
     YPAFCATCFP SEPGLVGPSG RAQAEDYALV SAVATLPKQV ADHYDNFQIY KALEAVSSCV
     RQTNGFVQRH APWKLNWESP VDAPWLGTVL HVALECLRVF GTLLQPVTPS LADRLLSRLG
     VSSTERSLGE LHFLSRFYGH PSPFEGRRLG PETGVLFPRL DQSRSWLVKA HKT