SYMM_MOUSE
ID SYMM_MOUSE Reviewed; 586 AA.
AC Q499X9; Q3T9W7; Q8BWR9; Q8BXY1;
DT 10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT 10-JAN-2006, sequence version 2.
DT 03-AUG-2022, entry version 129.
DE RecName: Full=Methionine--tRNA ligase, mitochondrial;
DE EC=6.1.1.10;
DE AltName: Full=Methionyl-tRNA synthetase 2;
DE AltName: Full=Mitochondrial methionyl-tRNA synthetase;
DE Short=MtMetRS;
DE Flags: Precursor;
GN Name=Mars2;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J, and NOD; TISSUE=Cerebellum, Liver, and Spleen;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=FVB/N-3; TISSUE=Mammary tumor;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain, Brown adipose tissue, Heart, Kidney, and Spleen;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-
CC methionyl-tRNA(Met); Xref=Rhea:RHEA:13481, Rhea:RHEA-COMP:9667,
CC Rhea:RHEA-COMP:9698, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:57844, ChEBI:CHEBI:78442, ChEBI:CHEBI:78530,
CC ChEBI:CHEBI:456215; EC=6.1.1.10;
CC -!- SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000250}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC31393.1; Type=Erroneous initiation; Evidence={ECO:0000305};
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DR EMBL; AK042888; BAC31393.1; ALT_INIT; mRNA.
DR EMBL; AK050196; BAC34119.1; -; mRNA.
DR EMBL; AK172240; BAE42903.1; -; mRNA.
DR EMBL; BC099669; AAH99669.1; -; mRNA.
DR CCDS; CCDS14963.1; -.
DR RefSeq; NP_780648.1; NM_175439.3.
DR AlphaFoldDB; Q499X9; -.
DR SMR; Q499X9; -.
DR BioGRID; 229348; 1.
DR STRING; 10090.ENSMUSP00000049770; -.
DR iPTMnet; Q499X9; -.
DR PhosphoSitePlus; Q499X9; -.
DR EPD; Q499X9; -.
DR MaxQB; Q499X9; -.
DR PaxDb; Q499X9; -.
DR PRIDE; Q499X9; -.
DR ProteomicsDB; 254839; -.
DR Antibodypedia; 50280; 123 antibodies from 23 providers.
DR DNASU; 212679; -.
DR Ensembl; ENSMUST00000061334; ENSMUSP00000049770; ENSMUSG00000046994.
DR GeneID; 212679; -.
DR KEGG; mmu:212679; -.
DR UCSC; uc007baj.1; mouse.
DR CTD; 92935; -.
DR MGI; MGI:2444136; Mars2.
DR VEuPathDB; HostDB:ENSMUSG00000046994; -.
DR eggNOG; KOG0436; Eukaryota.
DR GeneTree; ENSGT00550000075136; -.
DR HOGENOM; CLU_009710_9_0_1; -.
DR InParanoid; Q499X9; -.
DR OMA; SDMHGTP; -.
DR OrthoDB; 788159at2759; -.
DR PhylomeDB; Q499X9; -.
DR TreeFam; TF105709; -.
DR BioGRID-ORCS; 212679; 28 hits in 74 CRISPR screens.
DR ChiTaRS; Mars2; mouse.
DR PRO; PR:Q499X9; -.
DR Proteomes; UP000000589; Chromosome 1.
DR RNAct; Q499X9; protein.
DR Bgee; ENSMUSG00000046994; Expressed in spermatocyte and 126 other tissues.
DR ExpressionAtlas; Q499X9; baseline and differential.
DR Genevisible; Q499X9; MM.
DR GO; GO:0005759; C:mitochondrial matrix; ISO:MGI.
DR GO; GO:0005739; C:mitochondrion; HDA:MGI.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004825; F:methionine-tRNA ligase activity; ISO:MGI.
DR GO; GO:0006431; P:methionyl-tRNA aminoacylation; ISO:MGI.
DR CDD; cd07957; Anticodon_Ia_Met; 1.
DR CDD; cd00814; MetRS_core; 1.
DR Gene3D; 3.40.50.620; -; 1.
DR InterPro; IPR041872; Anticodon_Met.
DR InterPro; IPR014758; Met-tRNA_synth.
DR InterPro; IPR023457; Met-tRNA_synth_2.
DR InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR InterPro; IPR033911; MetRS_core.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR PANTHER; PTHR43326; PTHR43326; 1.
DR Pfam; PF19303; Anticodon_3; 1.
DR Pfam; PF09334; tRNA-synt_1g; 1.
DR PRINTS; PR01041; TRNASYNTHMET.
DR SUPFAM; SSF47323; SSF47323; 1.
DR TIGRFAMs; TIGR00398; metG; 1.
PE 1: Evidence at protein level;
KW Aminoacyl-tRNA synthetase; ATP-binding; Ligase; Mitochondrion;
KW Nucleotide-binding; Protein biosynthesis; Reference proteome;
KW Transit peptide.
FT TRANSIT 1..46
FT /note="Mitochondrion"
FT /evidence="ECO:0000255"
FT CHAIN 47..586
FT /note="Methionine--tRNA ligase, mitochondrial"
FT /id="PRO_0000045494"
FT MOTIF 45..55
FT /note="'HIGH' region"
FT MOTIF 340..344
FT /note="'KMSKS' region"
FT BINDING 343
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250"
FT CONFLICT 306
FT /note="L -> F (in Ref. 2; AAH99669)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 586 AA; 65805 MW; 639695ECEE842EFB CRC64;
MLRQCARWVL TRTRFGRGCR RYGSCSPSAS GDAGEARAYF TTPIFYVNAA PHIGHLYSAL
LADALCRHRR LRVPGSASTR FSTGTDEHGL KIQQAAATAG LAPIELCDRV SAQFLQLFRE
ADISSTDFIR TTEARHRVAV QHFWGVLEAR GLLYKGIYEG WYCASDECFL PEAKVTRQVG
PSGDPCPVSL ESGHPVSWTK EENYIFKLSQ FREPLQRWLG NNPQAITPEP FHQAVLQWLE
EELPDLSVSR RSSHLHWGIP VPGDDSQTIY VWLDALVNYL TVVGYPDADF KSWWPATSHI
IGKDILKFHA IYWPALLLGA GLRPPHRIYV HSHWTVSGQK MSKSLGNVVD PRTCLDRYTV
DGFRYFLLRQ GVPNWDCDYY DEKVVKLLDS ELADALGGLL NRCTAYRINP SGTYPSFCAA
CFPSEPGLTG PSVRVQAEDY ALVTAVATLP KLVAGYYNDF QIYKALEAVS SCVRQTNGFV
QRHAPWKLNW ESPEDAPWLG TVLHVALECL RVFGTLLQPV TPNLADKLLS RLGVSTTERG
LGELYFLPRF YGHPCPFEGR KLGPDTGLLF PRLDQSRTRL VKAHRT
