ID   SYMM_XENLA              Reviewed;         562 AA.
AC   Q7T0Z0;
DT   10-JAN-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-OCT-2003, sequence version 1.
DT   03-AUG-2022, entry version 92.
DE   RecName: Full=Methionine--tRNA ligase, mitochondrial;
DE            EC=6.1.1.10;
DE   AltName: Full=Methionyl-tRNA synthetase 2;
DE   AltName: Full=Mitochondrial methionyl-tRNA synthetase;
DE            Short=MtMetRS;
DE   Flags: Precursor;
GN   Name=mars2;
OS   Xenopus laevis (African clawed frog).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Amphibia;
OC   Batrachia; Anura; Pipoidea; Pipidae; Xenopodinae; Xenopus; Xenopus.
OX   NCBI_TaxID=8355;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Embryo;
RG   NIH - Xenopus Gene Collection (XGC) project;
RL   Submitted (AUG-2003) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-
CC         methionyl-tRNA(Met); Xref=Rhea:RHEA:13481, Rhea:RHEA-COMP:9667,
CC         Rhea:RHEA-COMP:9698, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:57844, ChEBI:CHEBI:78442, ChEBI:CHEBI:78530,
CC         ChEBI:CHEBI:456215; EC=6.1.1.10;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000305}.
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DR   EMBL; BC055977; AAH55977.1; -; mRNA.
DR   RefSeq; NP_001079838.1; NM_001086369.1.
DR   AlphaFoldDB; Q7T0Z0; -.
DR   SMR; Q7T0Z0; -.
DR   MaxQB; Q7T0Z0; -.
DR   DNASU; 379528; -.
DR   GeneID; 379528; -.
DR   KEGG; xla:379528; -.
DR   CTD; 379528; -.
DR   Xenbase; XB-GENE-5862169; mars2.L.
DR   OrthoDB; 788159at2759; -.
DR   Proteomes; UP000186698; Chromosome 8L.
DR   Bgee; 379528; Expressed in oocyte and 19 other tissues.
DR   GO; GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004825; F:methionine-tRNA ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0006431; P:methionyl-tRNA aminoacylation; IEA:InterPro.
DR   CDD; cd00814; MetRS_core; 1.
DR   Gene3D; 3.40.50.620; -; 1.
DR   InterPro; IPR041872; Anticodon_Met.
DR   InterPro; IPR014758; Met-tRNA_synth.
DR   InterPro; IPR023457; Met-tRNA_synth_2.
DR   InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR   InterPro; IPR033911; MetRS_core.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   PANTHER; PTHR43326; PTHR43326; 1.
DR   Pfam; PF19303; Anticodon_3; 1.
DR   Pfam; PF09334; tRNA-synt_1g; 1.
DR   PRINTS; PR01041; TRNASYNTHMET.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   TIGRFAMs; TIGR00398; metG; 1.
PE   2: Evidence at transcript level;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Ligase; Mitochondrion;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome;
KW   Transit peptide.
FT   TRANSIT         1..10
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           11..562
FT                   /note="Methionine--tRNA ligase, mitochondrial"
FT                   /id="PRO_0000045496"
FT   MOTIF           43..53
FT                   /note="'HIGH' region"
FT   MOTIF           332..336
FT                   /note="'KMSKS' region"
FT   BINDING         335
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   562 AA;  63461 MW;  A6C04967DEC299F4 CRC64;
     MLRSLALRTF ANILGLSSRS GSTAAMSRNA VASRPHLYTT PIFYVNAAPH LGHVYSALLA
     DVQHRYSAMC GIESKLSTGT DEHGMKVQQA ASALGLDPQT FCSTVSLQFR TIFDALDISY
     TDFVRTTEPR HIEAVSRFWM TLEEQGYIYK GTYEGWYCTS DEAFLSEGQT AEHTDFEGNK
     IRVSLESGHQ VHWVSEENYM FRLSSLRPAL LNWLQTEPVH PAPFLKLVHH WLEEELPDLS
     VSRQRSRLSW GIPVPSDSSH VIYVWLDALV NYLTAAGYPN PQLAPWGPST HLLGKDILRF
     HAIYWPAFLI AAGLPPPQKL LVHSHWTSEG TKMSKSLKNV VDPSDCIRRY TTDGLRYYLL
     RHGAPERDCD FTHRTARMLL NSELADALGG LLNRCTAPAI NPMQHFPKFQ YENFPVASRD
     QVHDLLGALQ ELPVEVDQWI KKFQVHKALE CIDACVRRSN AFFQSQAPWK LQRGVEKEAA
     LRDSVIYLTL EALRLYATLL HPAVPGLATV VLDRLGVPHK MRTLKKNTFL AATRGEICYF
     QAQTLGPDKG LLFPRLEKSE AF