BM23_DROME
ID BM23_DROME Reviewed; 117 AA.
AC Q9V8F5; Q8SY64;
DT 02-FEB-2004, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2002, sequence version 2.
DT 03-AUG-2022, entry version 111.
DE RecName: Full=Bomanin Bicipital 1 {ECO:0000312|FlyBase:FBgn0034328};
DE AltName: Full=Bomanin-23 {ECO:0000303|PubMed:25915418};
DE AltName: Full=Immune-induced peptide 23 {ECO:0000312|FlyBase:FBgn0034328};
DE Short=DIM-23 {ECO:0000312|FlyBase:FBgn0034328};
DE Short=DIM23 {ECO:0000303|PubMed:9736738};
DE Flags: Precursor;
GN Name=BomBc1 {ECO:0000312|FlyBase:FBgn0034328};
GN Synonyms=Bom23 {ECO:0000312|FlyBase:FBgn0034328},
GN IM23 {ECO:0000312|FlyBase:FBgn0034328};
GN ORFNames=CG15066 {ECO:0000312|FlyBase:FBgn0034328};
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227 {ECO:0000312|EMBL:AAF57712.2};
RN [1] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:10731132};
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [2] {ECO:0000305}
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [3] {ECO:0000305}
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley {ECO:0000269|PubMed:12537569};
RC TISSUE=Head {ECO:0000269|PubMed:12537569};
RX PubMed=12537569; DOI=10.1186/gb-2002-3-12-research0080;
RA Stapleton M., Carlson J.W., Brokstein P., Yu C., Champe M., George R.A.,
RA Guarin H., Kronmiller B., Pacleb J.M., Park S., Wan K.H., Rubin G.M.,
RA Celniker S.E.;
RT "A Drosophila full-length cDNA resource.";
RL Genome Biol. 3:RESEARCH0080.1-RESEARCH0080.8(2002).
RN [4] {ECO:0000305}
RP SUBCELLULAR LOCATION, TISSUE SPECIFICITY, INDUCTION BY BACTERIA, AND MASS
RP SPECTROMETRY.
RC STRAIN=Oregon-R {ECO:0000303|PubMed:9736738};
RC TISSUE=Hemolymph {ECO:0000303|PubMed:9736738};
RX PubMed=9736738; DOI=10.1073/pnas.95.19.11342;
RA Uttenweiler-Joseph S., Moniatte M., Lagueux M., van Dorsselaer A.,
RA Hoffmann J.A., Bulet P.;
RT "Differential display of peptides induced during the immune response of
RT Drosophila: a matrix-assisted laser desorption ionization time-of-flight
RT mass spectrometry study.";
RL Proc. Natl. Acad. Sci. U.S.A. 95:11342-11347(1998).
RN [5] {ECO:0000305}
RP PROTEIN SEQUENCE OF 21-117, DISULFIDE BONDS, SUBCELLULAR LOCATION, AND
RP TISSUE SPECIFICITY.
RC STRAIN=Oregon-R {ECO:0000303|Ref.5}; TISSUE=Hemolymph {ECO:0000303|Ref.5};
RA Bulet P., Charlet M., Ehret-Sabatier L.;
RL Submitted (JUL-2002) to UniProtKB.
RN [6]
RP FUNCTION.
RX PubMed=25915418; DOI=10.1371/journal.ppat.1004876;
RA Clemmons A.W., Lindsay S.A., Wasserman S.A.;
RT "An effector Peptide family required for Drosophila toll-mediated
RT immunity.";
RL PLoS Pathog. 11:E1004876-E1004876(2015).
RN [7]
RP FUNCTION.
RX PubMed=29920489; DOI=10.1159/000489831;
RA Lindsay S.A., Lin S.J.H., Wasserman S.A.;
RT "Short-Form Bomanins Mediate Humoral Immunity in Drosophila.";
RL J. Innate Immun. 10:306-314(2018).
CC -!- FUNCTION: Secreted immune-induced peptide induced by Toll signaling
CC (PubMed:9736738). Has a role in resistance to bacterial and fungal
CC infections (PubMed:25915418, PubMed:29920489).
CC {ECO:0000269|PubMed:25915418, ECO:0000269|PubMed:29920489,
CC ECO:0000269|PubMed:9736738}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000269|PubMed:29920489,
CC ECO:0000269|PubMed:9736738, ECO:0000269|Ref.5}.
CC -!- TISSUE SPECIFICITY: Hemolymph (at protein level).
CC {ECO:0000269|PubMed:29920489, ECO:0000269|PubMed:9736738,
CC ECO:0000269|Ref.5}.
CC -!- INDUCTION: By bacterial infection (at protein level) (PubMed:9736738).
CC Detected within 24 hours of infection (at protein level)
CC (PubMed:9736738). {ECO:0000269|PubMed:9736738}.
CC -!- MASS SPECTROMETRY: Mass=9482.41; Method=MALDI;
CC Evidence={ECO:0000269|PubMed:9736738};
CC -!- MISCELLANEOUS: Not induced after bacterial challenge in strains
CC carrying a loss-of-function mutation for Toll. Constitutively expressed
CC in Toll gain-of-function mutants. {ECO:0000269|PubMed:9736738}.
CC -!- SIMILARITY: Belongs to the bomanin family. {ECO:0000305}.
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DR EMBL; AE013599; AAF57712.2; -; Genomic_DNA.
DR EMBL; AY071762; AAL49384.1; -; mRNA.
DR RefSeq; NP_611318.2; NM_137474.5.
DR AlphaFoldDB; Q9V8F5; -.
DR IntAct; Q9V8F5; 8.
DR STRING; 7227.FBpp0085908; -.
DR PaxDb; Q9V8F5; -.
DR DNASU; 37099; -.
DR EnsemblMetazoa; FBtr0086729; FBpp0085908; FBgn0034328.
DR GeneID; 37099; -.
DR KEGG; dme:Dmel_CG15066; -.
DR CTD; 37099; -.
DR FlyBase; FBgn0034328; BomBc1.
DR VEuPathDB; VectorBase:FBgn0034328; -.
DR HOGENOM; CLU_2294530_0_0_1; -.
DR InParanoid; Q9V8F5; -.
DR OMA; NSPDAYP; -.
DR OrthoDB; 1553482at2759; -.
DR PhylomeDB; Q9V8F5; -.
DR BioGRID-ORCS; 37099; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 37099; -.
DR PRO; PR:Q9V8F5; -.
DR Proteomes; UP000000803; Chromosome 2R.
DR Bgee; FBgn0034328; Expressed in capitellum (Drosophila) and 13 other tissues.
DR ExpressionAtlas; Q9V8F5; baseline and differential.
DR Genevisible; Q9V8F5; DM.
DR GO; GO:0005576; C:extracellular region; IDA:UniProtKB.
DR GO; GO:0019731; P:antibacterial humoral response; IDA:UniProtKB.
DR GO; GO:0050830; P:defense response to Gram-positive bacterium; IEP:FlyBase.
DR GO; GO:0045087; P:innate immune response; IEA:UniProtKB-KW.
DR GO; GO:0009617; P:response to bacterium; HEP:FlyBase.
DR InterPro; IPR013172; Bomanin.
DR Pfam; PF08194; DIM; 1.
PE 1: Evidence at protein level;
KW Direct protein sequencing; Disulfide bond; Immunity; Innate immunity;
KW Reference proteome; Secreted; Signal.
FT SIGNAL 1..20
FT /evidence="ECO:0000255"
FT CHAIN 21..117
FT /note="Bomanin Bicipital 1"
FT /id="PRO_0000021508"
FT DISULFID 29..32
FT /evidence="ECO:0000269|Ref.5"
FT DISULFID 107..110
FT /evidence="ECO:0000269|Ref.5"
FT CONFLICT 66
FT /note="L -> P (in Ref. 5; AA sequence)"
FT /evidence="ECO:0000305"
FT CONFLICT 86..87
FT /note="AQ -> TR (in Ref. 5; AA sequence)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 117 AA; 11552 MW; DFFCF1230AFD6FF7 CRC64;
MKCLILSFAI FVVLASQATA GNVIIGGVCQ DCSPPVAENV VVGGQSYRTG RPGQGTVYIN
SPGAYLGALD GPIRRTGAGG GGGGGAQYPD GYSGRLPGGT YLHNKDCVGC SISGGGD
