ID   BM3R1_PRIMG             Reviewed;         192 AA.
AC   P43506;
DT   01-NOV-1995, integrated into UniProtKB/Swiss-Prot.
DT   01-NOV-1995, sequence version 1.
DT   25-MAY-2022, entry version 81.
DE   RecName: Full=HTH-type transcriptional repressor Bm3R1;
GN   Name=bm3R1;
OS   Priestia megaterium (Bacillus megaterium).
OC   Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Priestia.
OX   NCBI_TaxID=1404;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=1544926; DOI=10.1016/s0021-9258(18)42797-4;
RA   Shaw G.C., Fulco A.J.;
RT   "Barbiturate-mediated regulation of expression of the cytochrome P450BM-3
RT   gene of Bacillus megaterium by Bm3R1 protein.";
RL   J. Biol. Chem. 267:5515-5526(1992).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RX   PubMed=2544578; DOI=10.1016/s0021-9258(18)60416-8;
RA   Ruettinger R.T., Wen L.-P., Fulco A.J.;
RT   "Coding nucleotide, 5' regulatory, and deduced amino acid sequences of P-
RT   450BM-3, a single peptide cytochrome P-450:NADPH-P-450 reductase from
RT   Bacillus megaterium.";
RL   J. Biol. Chem. 264:10987-10995(1989).
RN   [3]
RP   CHARACTERIZATION.
RX   PubMed=8428974; DOI=10.1016/s0021-9258(18)53872-2;
RA   Shaw G.C., Fulco A.J.;
RT   "Inhibition by barbiturates of the binding of Bm3R1 repressor to its
RT   operator site on the barbiturate-inducible cytochrome P450BM-3 gene of
RT   Bacillus megaterium.";
RL   J. Biol. Chem. 268:2997-3004(1993).
CC   -!- FUNCTION: Negatively controls the expression of the cytochrome p450BM-3
CC       gene at the transcriptional level.
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DR   EMBL; S87512; AAB21757.1; -; Genomic_DNA.
DR   EMBL; J04832; AAA87601.1; -; Genomic_DNA.
DR   PIR; A42116; A42116.
DR   RefSeq; WP_013083972.1; NZ_UFSS01000002.1.
DR   AlphaFoldDB; P43506; -.
DR   SMR; P43506; -.
DR   PATRIC; fig|1404.33.peg.1857; -.
DR   GO; GO:0003677; F:DNA binding; IEA:UniProtKB-KW.
DR   InterPro; IPR032551; BscR_C.
DR   InterPro; IPR023772; DNA-bd_HTH_TetR-type_CS.
DR   InterPro; IPR009057; Homeobox-like_sf.
DR   InterPro; IPR001647; HTH_TetR.
DR   InterPro; IPR036271; Tet_transcr_reg_TetR-rel_C_sf.
DR   Pfam; PF16295; TetR_C_10; 1.
DR   Pfam; PF00440; TetR_N; 1.
DR   PRINTS; PR00455; HTHTETR.
DR   SUPFAM; SSF46689; SSF46689; 1.
DR   SUPFAM; SSF48498; SSF48498; 1.
DR   PROSITE; PS01081; HTH_TETR_1; 1.
DR   PROSITE; PS50977; HTH_TETR_2; 1.
PE   1: Evidence at protein level;
KW   DNA-binding; Repressor; Transcription; Transcription regulation.
FT   CHAIN           1..192
FT                   /note="HTH-type transcriptional repressor Bm3R1"
FT                   /id="PRO_0000070592"
FT   DOMAIN          5..65
FT                   /note="HTH tetR-type"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
FT   DNA_BIND        28..47
FT                   /note="H-T-H motif"
FT                   /evidence="ECO:0000255|PROSITE-ProRule:PRU00335"
SQ   SEQUENCE   192 AA;  21886 MW;  766AC6DD34944748 CRC64;
     MESTPTKQKA IFSASLLLFA ERGFDATTMP MIAENAKVGA GTIYRYFKNK ESLVNELFQQ
     HVNEFLQCIE SGLANERDGY RDGFHHIFEG MVTFTKNHPR ALGFIKTHSQ GTFLTEESRL
     AYQKLVEFVC TFFREGQKQG VIRNLPENAL IAILFGSFME VYEMIENDYL SLTDELLTGV
     EESLWAALSR QS