BM86_RHIMP
ID BM86_RHIMP Reviewed; 650 AA.
AC P20736;
DT 01-FEB-1991, integrated into UniProtKB/Swiss-Prot.
DT 01-FEB-1991, sequence version 1.
DT 03-AUG-2022, entry version 92.
DE RecName: Full=Glycoprotein antigen BM86;
DE AltName: Full=Protective antigen;
DE Flags: Precursor;
OS Rhipicephalus microplus (Cattle tick) (Boophilus microplus).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Chelicerata; Arachnida; Acari;
OC Parasitiformes; Ixodida; Ixodoidea; Ixodidae; Rhipicephalinae;
OC Rhipicephalus; Boophilus.
OX NCBI_TaxID=6941;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA], AND PARTIAL PROTEIN SEQUENCE.
RC TISSUE=Gut;
RX PubMed=2690068; DOI=10.1073/pnas.86.24.9657;
RA Rand K.N., Moore T., Sriskantha A., Spring K., Tellam R.L., Willadsen P.,
RA Cobon G.S.;
RT "Cloning and expression of a protective antigen from the cattle tick
RT Boophilus microplus.";
RL Proc. Natl. Acad. Sci. U.S.A. 86:9657-9661(1989).
RN [2]
RP PARTIAL PROTEIN SEQUENCE.
RX PubMed=2745982;
RA Willadsen P., Riding G.A., McKenna R.V., Kemp D.H., Tellam R.L.,
RA Nielsen J.N., Lahnstein J., Cobon G.S., Gough J.M.;
RT "Immunologic control of a parasitic arthropod. Identification of a
RT protective antigen from Boophilus microplus.";
RL J. Immunol. 143:1346-1351(1989).
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Lipid-anchor, GPI-
CC anchor {ECO:0000305}.
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DR EMBL; M29321; AAA30098.1; -; mRNA.
DR PIR; A34498; A34498.
DR AlphaFoldDB; P20736; -.
DR VEuPathDB; VectorBase:LOC119171703; -.
DR GO; GO:0031225; C:anchored component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR InterPro; IPR000742; EGF-like_dom.
DR SMART; SM00181; EGF; 4.
PE 1: Evidence at protein level;
KW Cell membrane; Direct protein sequencing; Disulfide bond; EGF-like domain;
KW Glycoprotein; GPI-anchor; Lipoprotein; Membrane; Repeat; Signal.
FT SIGNAL 1..19
FT CHAIN 20..627
FT /note="Glycoprotein antigen BM86"
FT /id="PRO_0000007486"
FT PROPEP 628..650
FT /note="Removed in mature form"
FT /evidence="ECO:0000250"
FT /id="PRO_0000007487"
FT DOMAIN 20..66
FT /note="EGF-like 1"
FT DOMAIN 67..104
FT /note="EGF-like 2"
FT DOMAIN 205..247
FT /note="EGF-like 3"
FT DOMAIN 251..292
FT /note="EGF-like 4"
FT DOMAIN 291..335
FT /note="EGF-like 5"
FT DOMAIN 482..530
FT /note="EGF-like 6"
FT DOMAIN 531..568
FT /note="EGF-like 7"
FT REGION 603..628
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT LIPID 627
FT /note="GPI-anchor amidated serine"
FT /evidence="ECO:0000250"
FT CARBOHYD 141
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 182
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 348
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 382
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 24..37
FT /evidence="ECO:0000250"
FT DISULFID 32..49
FT /evidence="ECO:0000250"
FT DISULFID 51..65
FT /evidence="ECO:0000250"
FT DISULFID 71..81
FT /evidence="ECO:0000250"
FT DISULFID 76..91
FT /evidence="ECO:0000250"
FT DISULFID 93..103
FT /evidence="ECO:0000250"
FT DISULFID 209..222
FT /evidence="ECO:0000250"
FT DISULFID 218..231
FT /evidence="ECO:0000250"
FT DISULFID 233..246
FT /evidence="ECO:0000250"
FT DISULFID 255..269
FT /evidence="ECO:0000250"
FT DISULFID 263..278
FT /evidence="ECO:0000250"
FT DISULFID 280..291
FT /evidence="ECO:0000250"
FT DISULFID 295..307
FT /evidence="ECO:0000250"
FT DISULFID 300..316
FT /evidence="ECO:0000250"
FT DISULFID 318..334
FT /evidence="ECO:0000250"
FT DISULFID 486..500
FT /evidence="ECO:0000250"
FT DISULFID 492..516
FT /evidence="ECO:0000250"
FT DISULFID 518..529
FT /evidence="ECO:0000250"
FT DISULFID 535..550
FT /evidence="ECO:0000250"
FT DISULFID 543..559
FT /evidence="ECO:0000250"
FT DISULFID 561..567
FT /evidence="ECO:0000250"
FT VARIANT 235..237
FT /note="SGS -> RAF"
FT VARIANT 507
FT /note="F -> C"
SQ SEQUENCE 650 AA; 71722 MW; 5DED71E354D8312A CRC64;
MRGIALFVAA VSLIVEGTAE SSICSDFGNE FCRNAECEVV PGAEDDFVCK CPRDNMYFNA
AEKQCEYKDT CKTRECSYGR CVESNPSKAS CVCEASDDLT LQCKIKNDYA TDCRNRGGTA
KLRTDGFIGA TCDCGEWGAM NMTTRNCVPT TCLRPDLTCK DLCEKNLLQR DSRCCQGWNT
ANCSAAPPAD SYCSPGSPKG PDGQCINACK TKEAGFVCKH GCRSTGKAYE CTCPSGSTVA
EDGITCKSIS HTVSCTAEQK QTCRPTEDCR VHKGTVLCEC PWNQHLVGDT CISDCVDKKC
HEEFMDCGVY MNRQSCYCPW KSRKPGPNVN INECLLNEYY YTVSFTPNIS FDSDHCKWYE
DRVLEAIRTS IGKEVFKVEI LNCTQDIKAR LIAEKPLSKH VLRKLQACEH PIGEWCMMYP
KLLIKKNSAT EIEEENLCDS LLKDQEAAYK GQNKCVKVDN LFWFQCADGY TTTYEMTRGR
LRRSVCKAGV SCNENEQSEC ADKGQIFVYE NGKANCQCPP DTKPGEIGCI ERTTCNPKEI
QECQDKKLEC VYKNHKAECE CPDDHECYRE PAKDSCSEED NGKCQSSGQR CVIENGKAVC
KEKSEATTAA TTTTKAKDKD PDPGKSSAAA VSATGLLLLL AATSVTAASL
