ID   SYM_RICPR               Reviewed;         508 AA.
AC   Q9ZCP0;
DT   30-MAY-2000, integrated into UniProtKB/Swiss-Prot.
DT   01-MAY-1999, sequence version 1.
DT   03-AUG-2022, entry version 118.
DE   RecName: Full=Methionine--tRNA ligase;
DE            EC=6.1.1.10;
DE   AltName: Full=Methionyl-tRNA synthetase;
DE            Short=MetRS;
GN   Name=metG; Synonyms=metS; OrderedLocusNames=RP683;
OS   Rickettsia prowazekii (strain Madrid E).
OC   Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales;
OC   Rickettsiaceae; Rickettsieae; Rickettsia; typhus group.
OX   NCBI_TaxID=272947;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=Madrid E;
RX   PubMed=9823893; DOI=10.1038/24094;
RA   Andersson S.G.E., Zomorodipour A., Andersson J.O., Sicheritz-Ponten T.,
RA   Alsmark U.C.M., Podowski R.M., Naeslund A.K., Eriksson A.-S., Winkler H.H.,
RA   Kurland C.G.;
RT   "The genome sequence of Rickettsia prowazekii and the origin of
RT   mitochondria.";
RL   Nature 396:133-140(1998).
CC   -!- FUNCTION: Is required not only for elongation of protein synthesis but
CC       also for the initiation of all mRNA translation through initiator
CC       tRNA(fMet) aminoacylation. {ECO:0000250}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-methionine + tRNA(Met) = AMP + diphosphate + L-
CC         methionyl-tRNA(Met); Xref=Rhea:RHEA:13481, Rhea:RHEA-COMP:9667,
CC         Rhea:RHEA-COMP:9698, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:57844, ChEBI:CHEBI:78442, ChEBI:CHEBI:78530,
CC         ChEBI:CHEBI:456215; EC=6.1.1.10;
CC   -!- SUBUNIT: Monomer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       MetG type 2B subfamily. {ECO:0000305}.
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DR   EMBL; AJ235272; CAA15120.1; -; Genomic_DNA.
DR   PIR; F71674; F71674.
DR   RefSeq; NP_221044.1; NC_000963.1.
DR   RefSeq; WP_004596363.1; NC_000963.1.
DR   AlphaFoldDB; Q9ZCP0; -.
DR   SMR; Q9ZCP0; -.
DR   STRING; 272947.RP683; -.
DR   EnsemblBacteria; CAA15120; CAA15120; CAA15120.
DR   GeneID; 57569808; -.
DR   KEGG; rpr:RP683; -.
DR   PATRIC; fig|272947.5.peg.705; -.
DR   eggNOG; COG0143; Bacteria.
DR   HOGENOM; CLU_009710_9_2_5; -.
DR   OMA; SDMHGTP; -.
DR   Proteomes; UP000002480; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004825; F:methionine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006431; P:methionyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   CDD; cd07957; Anticodon_Ia_Met; 1.
DR   CDD; cd00814; MetRS_core; 1.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_01228; Met_tRNA_synth_type2; 1.
DR   InterPro; IPR041872; Anticodon_Met.
DR   InterPro; IPR014758; Met-tRNA_synth.
DR   InterPro; IPR023457; Met-tRNA_synth_2.
DR   InterPro; IPR015413; Methionyl/Leucyl_tRNA_Synth.
DR   InterPro; IPR033911; MetRS_core.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   PANTHER; PTHR43326; PTHR43326; 1.
DR   Pfam; PF19303; Anticodon_3; 1.
DR   Pfam; PF09334; tRNA-synt_1g; 2.
DR   PRINTS; PR01041; TRNASYNTHMET.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   TIGRFAMs; TIGR00398; metG; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..508
FT                   /note="Methionine--tRNA ligase"
FT                   /id="PRO_0000139238"
FT   MOTIF           12..22
FT                   /note="'HIGH' region"
FT   MOTIF           295..299
FT                   /note="'KMSKS' region"
FT   BINDING         298
FT                   /ligand="ATP"
FT                   /ligand_id="ChEBI:CHEBI:30616"
FT                   /evidence="ECO:0000250"
SQ   SEQUENCE   508 AA;  58592 MW;  C781AB4C8A674205 CRC64;
     MKNTYYITTP IYYVNDVAHI GHAYTSVASD VIARFMRFCG KDVMFLTGTD EHGQKVEKAA
     INQNIDPQTF TDKTSQNFRD LMVAMNISND DFIRTTENRH KKAVAVFWKK LLDNGAIYEG
     FYEGWYSVRD EAFYDESEIN EDKLAPTGAP VEWVKEPSYF FNLAKWQDKL LEFYELNPDF
     VRPISRRNEV ISFIKSGLKD LSISRTTFHW GIKVPNNRKH VIYVWLDALV NYISALGYPD
     KQSNYAKFWP ANLQIVGKDI LRFHAVYWPA FLMAAEIPLP KAIMVHGWWT NEGQKISKSL
     GNTIDPITLI DEFGVDQVRY FLMREVIFGA DANFARNNLI TRINSELSNK IGNLLHRIVS
     FVYKNNDAKV PLIKSGVIDK IYELPILKTA IKFAQENILL MDKTEINKIL ENIINLAEDA
     NIYITNEAPW NLKTTDPDKM LEVLYSLLEV LRYIAIMLQP FVPNSANKML DQLGVSKEER
     LFKHLVRDHA LKAGSNILEP SIIFPKFN