ID   BMBL_DANRE              Reviewed;         612 AA.
AC   I6V1W0;
DT   06-FEB-2013, integrated into UniProtKB/Swiss-Prot.
DT   03-OCT-2012, sequence version 1.
DT   03-AUG-2022, entry version 39.
DE   RecName: Full=Protein brambleberry;
DE   Flags: Precursor;
GN   Name=bmb;
OS   Danio rerio (Zebrafish) (Brachydanio rerio).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi;
OC   Actinopterygii; Neopterygii; Teleostei; Ostariophysi; Cypriniformes;
OC   Danionidae; Danioninae; Danio.
OX   NCBI_TaxID=7955;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [MRNA], SUBCELLULAR LOCATION, FUNCTION, AND DISRUPTION
RP   PHENOTYPE.
RC   TISSUE=Ovary;
RX   PubMed=22863006; DOI=10.1016/j.cell.2012.05.048;
RA   Abrams E.W., Zhang H., Marlow F.L., Kapp L., Lu S., Mullins M.C.;
RT   "Dynamic assembly of brambleberry mediates nuclear envelope fusion during
RT   early development.";
RL   Cell 150:521-532(2012).
CC   -!- FUNCTION: Required for nuclear membrane fusion during karyogamy.
CC       {ECO:0000269|PubMed:22863006}.
CC   -!- SUBCELLULAR LOCATION: Nucleus membrane {ECO:0000269|PubMed:22863006};
CC       Multi-pass membrane protein {ECO:0000269|PubMed:22863006}. Note=During
CC       metaphase, localizes near the mitotic spindle region, and its
CC       localization shifts to the chromosomes as they reach the end of the
CC       spindle. During karyomere fusion, detected in prominent puncta, mainly
CC       at karyomere-karyomere interfaces corresponding to putative fusion
CC       sites.
CC   -!- DISRUPTION PHENOTYPE: Embryos arrest development shortly after the
CC       midblastula transition. During cleavage (2-, 64-, and 1,000-cell-stage
CC       embryos) all blastomeres of mutant embryos contain morphologically
CC       abnormal nuclei that appeared fragmented in formation of multiple
CC       micronuclei. {ECO:0000269|PubMed:22863006}.
CC   -!- MISCELLANEOUS: Has a similar domain organization and 27% homology to
CC       yeast kar5. {ECO:0000305|PubMed:22863006}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; JX073281; AFN20270.1; -; mRNA.
DR   RefSeq; NP_001257483.1; NM_001270554.1.
DR   AlphaFoldDB; I6V1W0; -.
DR   SMR; I6V1W0; -.
DR   STRING; 7955.ENSDARP00000126692; -.
DR   Ensembl; ENSDART00000152329; ENSDARP00000126692; ENSDARG00000096603.
DR   GeneID; 559540; -.
DR   KEGG; dre:559540; -.
DR   CTD; 559540; -.
DR   ZFIN; ZDB-GENE-030131-5577; bmb.
DR   eggNOG; ENOG502QRBT; Eukaryota.
DR   GeneTree; ENSGT00740000115898; -.
DR   HOGENOM; CLU_027987_0_0_1; -.
DR   InParanoid; I6V1W0; -.
DR   OMA; MSEFLQY; -.
DR   OrthoDB; 1140613at2759; -.
DR   PRO; PR:I6V1W0; -.
DR   Proteomes; UP000000437; Genome assembly.
DR   Proteomes; UP000814640; Chromosome 25.
DR   Bgee; ENSDARG00000096603; Expressed in mature ovarian follicle and 25 other tissues.
DR   ExpressionAtlas; I6V1W0; baseline and differential.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031965; C:nuclear membrane; IEA:UniProtKB-SubCell.
DR   GO; GO:0061472; P:karyomere membrane fusion; IMP:ZFIN.
DR   GO; GO:0007344; P:pronuclear fusion; IMP:UniProtKB.
DR   InterPro; IPR040346; GEX1/Brambleberry.
DR   PANTHER; PTHR33538; PTHR33538; 1.
PE   2: Evidence at transcript level;
KW   Karyogamy; Membrane; Nucleus; Reference proteome; Signal; Transmembrane;
KW   Transmembrane helix.
FT   SIGNAL          1..20
FT                   /evidence="ECO:0000255"
FT   CHAIN           21..612
FT                   /note="Protein brambleberry"
FT                   /id="PRO_0000420971"
FT   TRANSMEM        364..384
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        417..437
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   REGION          452..476
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   REGION          555..574
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
SQ   SEQUENCE   612 AA;  68061 MW;  FD2522A47445E751 CRC64;
     MALWFVLLWV SSLQYAEVEA FFDWLKKADP APTPPPAESI VPILLHGEAP AFEMSVVDEK
     FLAEAKQMEL SPLDSCHFRV VAQLKATCSG LSEEQLAKLG VALFNCQSEV EGRRTYPCTE
     EMSIKECTAD MDSDTWNAYH IVSNRARSVC YATRQQHFRK RAELTVNALI STATSQLDAM
     KDLKEGQKEL RDMTAASLDK LLEGHGALQI QQGALKEGQE QLDASISENL QRLAQEKALI
     STGQQLVAQL IQGITQRMEN VSGQLKDQTA EVQEGHQAIL EDLAVVRGSA QDIYEKMELN
     LNGFLQQQNT TAHFYTELMR KLELMNGTLG YMLTYLDNMQ TRLEDRLHMI QGYLGWAGLS
     LRALWTCVMH AGYFLLCAVL LSFLQCTTFS RVTLLLSVPI NAIAEINQQA ALDLISLTLL
     LFTLSLGRWF VLQLLWALSK IKGRTCSRPP HLSIYPPKEK TPEKQHEFGE KCPASSSTPV
     QSDPVCDLEV ESFMMGDPCV LGVSPSRCPP KFSHHHLGGT PNHSTPRLKS RHSIAATELD
     NIPQRNLGVF LETVNRSRSS SPNQSLASSS SFSGRSLCSG ITRLGQPCKK RAVVGQDYCR
     VHEGGHTSYS RL