ID   SYN1_LACPL              Reviewed;         431 AA.
AC   Q88Y40; F9UMF1;
DT   31-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 110.
DE   RecName: Full=Asparagine--tRNA ligase 1;
DE            EC=6.1.1.22;
DE   AltName: Full=Asparaginyl-tRNA synthetase 1;
DE            Short=AsnRS 1;
GN   Name=asnS1; OrderedLocusNames=lp_0956;
OS   Lactiplantibacillus plantarum (strain ATCC BAA-793 / NCIMB 8826 / WCFS1)
OS   (Lactobacillus plantarum).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactiplantibacillus.
OX   NCBI_TaxID=220668;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1;
RX   PubMed=12566566; DOI=10.1073/pnas.0337704100;
RA   Kleerebezem M., Boekhorst J., van Kranenburg R., Molenaar D., Kuipers O.P.,
RA   Leer R., Tarchini R., Peters S.A., Sandbrink H.M., Fiers M.W.E.J.,
RA   Stiekema W., Klein Lankhorst R.M., Bron P.A., Hoffer S.M.,
RA   Nierop Groot M.N., Kerkhoven R., De Vries M., Ursing B., De Vos W.M.,
RA   Siezen R.J.;
RT   "Complete genome sequence of Lactobacillus plantarum WCFS1.";
RL   Proc. Natl. Acad. Sci. U.S.A. 100:1990-1995(2003).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA], AND GENOME REANNOTATION.
RC   STRAIN=ATCC BAA-793 / NCIMB 8826 / WCFS1;
RX   PubMed=22156394; DOI=10.1128/jb.06275-11;
RA   Siezen R.J., Francke C., Renckens B., Boekhorst J., Wels M.,
RA   Kleerebezem M., van Hijum S.A.;
RT   "Complete resequencing and reannotation of the Lactobacillus plantarum
RT   WCFS1 genome.";
RL   J. Bacteriol. 194:195-196(2012).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + H(+) + L-
CC         asparaginyl-tRNA(Asn); Xref=Rhea:RHEA:11180, Rhea:RHEA-COMP:9659,
CC         Rhea:RHEA-COMP:9674, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:58048, ChEBI:CHEBI:78442,
CC         ChEBI:CHEBI:78515, ChEBI:CHEBI:456215; EC=6.1.1.22;
CC   -!- SUBUNIT: Homodimer. {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000305}.
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DR   EMBL; AL935263; CCC78390.1; -; Genomic_DNA.
DR   RefSeq; WP_011101226.1; NC_004567.2.
DR   RefSeq; YP_004888904.1; NC_004567.2.
DR   AlphaFoldDB; Q88Y40; -.
DR   SMR; Q88Y40; -.
DR   STRING; 220668.lp_0956; -.
DR   EnsemblBacteria; CCC78390; CCC78390; lp_0956.
DR   KEGG; lpl:lp_0956; -.
DR   PATRIC; fig|220668.9.peg.813; -.
DR   eggNOG; COG0017; Bacteria.
DR   HOGENOM; CLU_004553_2_0_9; -.
DR   OMA; CKQHTVR; -.
DR   PhylomeDB; Q88Y40; -.
DR   BioCyc; LPLA220668:G1GW0-826-MON; -.
DR   Proteomes; UP000000432; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004816; F:asparagine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006421; P:asparaginyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.30.930.10; -; 1.
DR   HAMAP; MF_00534; Asn_tRNA_synth; 1.
DR   InterPro; IPR004364; Aa-tRNA-synt_II.
DR   InterPro; IPR006195; aa-tRNA-synth_II.
DR   InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR   InterPro; IPR004522; Asn-tRNA-ligase.
DR   InterPro; IPR002312; Asp/Asn-tRNA-synth_IIb.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   Pfam; PF00152; tRNA-synt_2; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   PRINTS; PR01042; TRNASYNTHASP.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF55681; SSF55681; 1.
DR   TIGRFAMs; TIGR00457; asnS; 1.
DR   PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..431
FT                   /note="Asparagine--tRNA ligase 1"
FT                   /id="PRO_0000176418"
SQ   SEQUENCE   431 AA;  48989 MW;  C9CB629A1E5EEEA4 CRC64;
     METISIINAA DHVNEQVKIG VWLSNKRSSG KIAFLQLRDG TATFQGVALK NELGETAFAQ
     LTDLAQETSL WVTGTIHADT RSPFGYELAI SQFEVVGTSH DYPITPKDHG IDFLLDHRHL
     WLRSSRPNAI MRIRNEVIRA VYEFFNDRGF IKIDAPILTG SAPEGTTELF KTDYFGQDAY
     LSQSGQLYGE AGAMAFGKIF TFGPTFRAEK SKTRRHLTEF WQLEPEMAWM HQDESLEVQE
     QLVAYLGQAV IDHCPHELAV LDRDVATLQK YTKLPFPRVS YDDAITLLQA NGFDVDWGVD
     FGSPEETFLA EHFDQPVFVL NYPKAIKAFY MQAHPTRDDV VIAADLLAPE GYGEIIGGSE
     RSTDYDYLKE RLLASGQDLQ DYEWYLDLRK YGSVPHSGFG MGLERFLAWI TLQDHVRETI
     PFPRMLHRIY P