ID   SYNC_ENTBH              Reviewed;         458 AA.
AC   A9CTP8;
DT   03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT   05-FEB-2008, sequence version 1.
DT   25-MAY-2022, entry version 62.
DE   RecName: Full=Probable asparagine--tRNA ligase, cytoplasmic;
DE            EC=6.1.1.22;
DE   AltName: Full=Asparaginyl-tRNA synthetase;
DE            Short=AsnRS;
GN   ORFNames=EBI_22533;
OS   Enterocytozoon bieneusi (strain H348) (Microsporidian parasite).
OC   Eukaryota; Fungi; Fungi incertae sedis; Microsporidia; Enterocytozoonidae;
OC   Enterocytozoon.
OX   NCBI_TaxID=481877;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=H348;
RX   PubMed=18060071; DOI=10.1371/journal.pone.0001277;
RA   Corradi N., Akiyoshi D.E., Morrison H.G., Feng X., Weiss L.M., Tzipori S.,
RA   Keeling P.J.;
RT   "Patterns of genome evolution among the microsporidian parasites
RT   Encephalitozoon cuniculi, Antonospora locustae and Enterocytozoon
RT   bieneusi.";
RL   PLoS ONE 2:E1277-E1277(2007).
RN   [2]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=H348;
RX   PubMed=19132089; DOI=10.1371/journal.ppat.1000261;
RA   Akiyoshi D.E., Morrison H.G., Lei S., Feng X., Zhang Q., Corradi N.,
RA   Mayanja H., Tumwine J.K., Keeling P.J., Weiss L.M., Tzipori S.;
RT   "Genomic survey of the non-cultivatable opportunistic human pathogen,
RT   Enterocytozoon bieneusi.";
RL   PLoS Pathog. 5:E1000261-E1000261(2009).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + H(+) + L-
CC         asparaginyl-tRNA(Asn); Xref=Rhea:RHEA:11180, Rhea:RHEA-COMP:9659,
CC         Rhea:RHEA-COMP:9674, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:58048, ChEBI:CHEBI:78442,
CC         ChEBI:CHEBI:78515, ChEBI:CHEBI:456215; EC=6.1.1.22;
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000250}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000305}.
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DR   EMBL; ABGB01000005; EDQ31040.1; -; Genomic_DNA.
DR   RefSeq; XP_001827945.1; XM_001827893.1.
DR   AlphaFoldDB; A9CTP8; -.
DR   SMR; A9CTP8; -.
DR   STRING; 481877.A9CTP8; -.
DR   EnsemblFungi; EDQ31040; EDQ31040; EBI_22533.
DR   VEuPathDB; MicrosporidiaDB:EBI_22533; -.
DR   HOGENOM; CLU_004553_2_10_1; -.
DR   InParanoid; A9CTP8; -.
DR   Proteomes; UP000001742; Unassembled WGS sequence.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004816; F:asparagine-tRNA ligase activity; IEA:UniProtKB-EC.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0006421; P:asparaginyl-tRNA aminoacylation; IEA:InterPro.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.30.930.10; -; 1.
DR   InterPro; IPR004364; Aa-tRNA-synt_II.
DR   InterPro; IPR006195; aa-tRNA-synth_II.
DR   InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR   InterPro; IPR004522; Asn-tRNA-ligase.
DR   InterPro; IPR002312; Asp/Asn-tRNA-synth_IIb.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   Pfam; PF00152; tRNA-synt_2; 1.
DR   PRINTS; PR01042; TRNASYNTHASP.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF55681; SSF55681; 1.
DR   TIGRFAMs; TIGR00457; asnS; 1.
DR   PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..458
FT                   /note="Probable asparagine--tRNA ligase, cytoplasmic"
FT                   /id="PRO_0000388389"
SQ   SEQUENCE   458 AA;  53283 MW;  EF35AB29FF8AC27E CRC64;
     MKLQDNLKSI KKELERIKVE NWSKENIDKN TYRYIRLFDI DDSLMNQKIC FFGWVKSSRS
     SSKISFIELI SQFKIIKCVI PTKVKLTDHT TLKVWGIMKP NNGNDEHQFE IDVQAYEVYG
     GYQAPSFPLN IHSDKDTLLD LAHLGLRMPH RILFLQAQNE LLKALRKFYW NNNYTEITPP
     TLVQTQVEGG ATLFKLNYYD QPAYLTQSSQ LYLETVAPVV GKAFCIMPSY RAEKSKTSRH
     LSEFTHVEAE LVDIQFDELM DQIEQLIRST INEFYKNILP EIKKIDNDFQ PVVLSDKQFK
     KITYEDAIHF LIAQNHKKTD NTDYQLGDDI SDASEKFLLE TYGENQPIFL IRFPTDHKPF
     YVAKDQYGTQ TCDLLFPGIG EIVGGSMRET NYNNLLDGFK RENIDHEPYS WYLDMAKFGP
     SPHGGYGLGF ERLLMCLMKY KSVDQSTLYC RKPSRCTP