SYNPR_MOUSE
ID SYNPR_MOUSE Reviewed; 265 AA.
AC Q8BGN8; Q8C5J4; Q9DB89;
DT 10-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2003, sequence version 1.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Synaptoporin;
GN Name=Synpr;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
RC STRAIN=C57BL/6J; TISSUE=Cerebellum, Olfactory bulb, and Spinal cord;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC TISSUE=Eye;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [3]
RP PROTEIN SEQUENCE OF 150-159 AND 210-217, AND IDENTIFICATION BY MASS
RP SPECTROMETRY.
RC STRAIN=C57BL/6J; TISSUE=Brain;
RA Lubec G., Kang S.U.;
RL Submitted (APR-2007) to UniProtKB.
RN [4]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brain;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- FUNCTION: Intrinsic membrane protein of small synaptic vesicles.
CC Probable vesicular channel protein (By similarity). {ECO:0000250}.
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle, secretory vesicle, synaptic
CC vesicle membrane {ECO:0000250}; Multi-pass membrane protein
CC {ECO:0000250}. Synapse, synaptosome {ECO:0000250}.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=Q8BGN8-1; Sequence=Displayed;
CC Name=2;
CC IsoId=Q8BGN8-2; Sequence=VSP_008550;
CC -!- SIMILARITY: Belongs to the synaptophysin/synaptobrevin family.
CC {ECO:0000305}.
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DR EMBL; AK005132; BAB23831.1; -; mRNA.
DR EMBL; AK032442; BAC27871.1; -; mRNA.
DR EMBL; AK049661; BAC33864.1; -; mRNA.
DR EMBL; AK078223; BAC37181.1; -; mRNA.
DR EMBL; BC026512; AAH26512.1; -; mRNA.
DR CCDS; CCDS26821.1; -. [Q8BGN8-2]
DR CCDS; CCDS49402.1; -. [Q8BGN8-1]
DR RefSeq; NP_001156504.1; NM_001163032.1. [Q8BGN8-1]
DR RefSeq; NP_082328.3; NM_028052.4. [Q8BGN8-2]
DR AlphaFoldDB; Q8BGN8; -.
DR STRING; 10090.ENSMUSP00000064986; -.
DR TCDB; 9.B.130.1.1; the tetraspan vesicle membrane protein (tvp) family.
DR GlyGen; Q8BGN8; 2 sites.
DR iPTMnet; Q8BGN8; -.
DR PhosphoSitePlus; Q8BGN8; -.
DR SwissPalm; Q8BGN8; -.
DR MaxQB; Q8BGN8; -.
DR PaxDb; Q8BGN8; -.
DR PeptideAtlas; Q8BGN8; -.
DR PRIDE; Q8BGN8; -.
DR ProteomicsDB; 254739; -. [Q8BGN8-1]
DR ProteomicsDB; 254740; -. [Q8BGN8-2]
DR Antibodypedia; 31743; 168 antibodies from 23 providers.
DR DNASU; 72003; -.
DR Ensembl; ENSMUST00000070323; ENSMUSP00000064986; ENSMUSG00000056296. [Q8BGN8-2]
DR Ensembl; ENSMUST00000112656; ENSMUSP00000108275; ENSMUSG00000056296. [Q8BGN8-1]
DR GeneID; 72003; -.
DR KEGG; mmu:72003; -.
DR UCSC; uc007sfy.2; mouse. [Q8BGN8-2]
DR UCSC; uc007sga.2; mouse. [Q8BGN8-1]
DR CTD; 132204; -.
DR MGI; MGI:1919253; Synpr.
DR VEuPathDB; HostDB:ENSMUSG00000056296; -.
DR eggNOG; ENOG502QT4W; Eukaryota.
DR GeneTree; ENSGT01030000234637; -.
DR HOGENOM; CLU_064642_0_1_1; -.
DR InParanoid; Q8BGN8; -.
DR OMA; NHGGYNQ; -.
DR OrthoDB; 1446480at2759; -.
DR PhylomeDB; Q8BGN8; -.
DR TreeFam; TF315804; -.
DR BioGRID-ORCS; 72003; 1 hit in 71 CRISPR screens.
DR ChiTaRS; Synpr; mouse.
DR PRO; PR:Q8BGN8; -.
DR Proteomes; UP000000589; Chromosome 14.
DR RNAct; Q8BGN8; protein.
DR Bgee; ENSMUSG00000056296; Expressed in habenula and 135 other tissues.
DR ExpressionAtlas; Q8BGN8; baseline and differential.
DR Genevisible; Q8BGN8; MM.
DR GO; GO:0070161; C:anchoring junction; IEA:UniProtKB-KW.
DR GO; GO:0030285; C:integral component of synaptic vesicle membrane; ISO:MGI.
DR GO; GO:0043005; C:neuron projection; IEA:UniProtKB-KW.
DR GO; GO:0030672; C:synaptic vesicle membrane; IBA:GO_Central.
DR InterPro; IPR008253; Marvel.
DR InterPro; IPR001285; Synaptophysin/porin.
DR PANTHER; PTHR10306; PTHR10306; 1.
DR Pfam; PF01284; MARVEL; 1.
DR PRINTS; PR00220; SYNAPTOPHYSN.
DR PROSITE; PS51225; MARVEL; 1.
DR PROSITE; PS00604; SYNAPTOP; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasmic vesicle; Direct protein sequencing;
KW Glycoprotein; Membrane; Phosphoprotein; Reference proteome; Repeat;
KW Synapse; Synaptosome; Transmembrane; Transmembrane helix.
FT CHAIN 1..265
FT /note="Synaptoporin"
FT /id="PRO_0000179158"
FT TOPO_DOM 1..4
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 5..25
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 26..81
FT /note="Vesicular"
FT /evidence="ECO:0000255"
FT TRANSMEM 82..102
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 103..114
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 115..135
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 136..177
FT /note="Vesicular"
FT /evidence="ECO:0000255"
FT TRANSMEM 178..198
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 199..265
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 1..202
FT /note="MARVEL"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00581"
FT REPEAT 210..214
FT /note="1"
FT REPEAT 222..226
FT /note="2"
FT REPEAT 227..231
FT /note="3"
FT REPEAT 232..236
FT /note="4"
FT REPEAT 238..242
FT /note="5"
FT REGION 210..242
FT /note="5 X approximate repeats"
FT REGION 221..265
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT MOD_RES 212
FT /note="Phosphoserine"
FT /evidence="ECO:0000250|UniProtKB:P22831"
FT CARBOHYD 33
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 38
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..8
FT /note="MCMVIFAP -> MDPVSQVASAGTFRALKEPLAFLRALEL (in isoform
FT 2)"
FT /evidence="ECO:0000303|PubMed:15489334,
FT ECO:0000303|PubMed:16141072"
FT /id="VSP_008550"
FT CONFLICT 230
FT /note="E -> K (in Ref. 1; BAC37181)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 265 AA; 29228 MW; 81DD473A422815E5 CRC64;
MCMVIFAPLF AMFAFATCGG YSGGLRLSVD CVNKTESNLS IDIAFAYPFR LQQVTFEVPT
CEGKEQQKLA LVGDSSSSAE FFVTVAVFAF LYSLAATVVY IFFQNKYREN NRGPLIDFIV
TVVFSFLWLV GSSAWAKGLS DVKVATDPKE VLLLMSACKQ PSNKCMAVHS PVMSSLNTSV
VFGFLNFILW AGNIWFVFKE TGWHSSGQRY LSDPMEKHSS SYNQGRYNQE SYGSSGGYSQ
QANLGPTSDE FGQQPSGPTS FNNQI
