ID   BMGDS_NOSS1             Reviewed;         468 AA.
AC   Q8YMK0;
DT   19-FEB-2014, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2002, sequence version 1.
DT   25-MAY-2022, entry version 84.
DE   RecName: Full=Beta-monoglucosyldiacylglycerol synthase;
DE            Short=Beta-MGS;
DE            Short=MGlcDAG synthase;
DE            EC=2.4.1.336 {ECO:0000269|PubMed:16714404};
DE   AltName: Full=UDP-glucose:1,2-diacylglycerol 3-beta-D-glucosyltransferase;
GN   OrderedLocusNames=all4933;
OS   Nostoc sp. (strain PCC 7120 / SAG 25.82 / UTEX 2576).
OC   Bacteria; Cyanobacteria; Nostocales; Nostocaceae; Nostoc.
OX   NCBI_TaxID=103690;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=PCC 7120 / SAG 25.82 / UTEX 2576;
RX   PubMed=11759840; DOI=10.1093/dnares/8.5.205;
RA   Kaneko T., Nakamura Y., Wolk C.P., Kuritz T., Sasamoto S., Watanabe A.,
RA   Iriguchi M., Ishikawa A., Kawashima K., Kimura T., Kishida Y., Kohara M.,
RA   Matsumoto M., Matsuno A., Muraki A., Nakazaki N., Shimpo S., Sugimoto M.,
RA   Takazawa M., Yamada M., Yasuda M., Tabata S.;
RT   "Complete genomic sequence of the filamentous nitrogen-fixing
RT   cyanobacterium Anabaena sp. strain PCC 7120.";
RL   DNA Res. 8:205-213(2001).
RN   [2]
RP   FUNCTION, CATALYTIC ACTIVITY, SUBSTRATE SPECIFICITY, AND COFACTOR.
RC   STRAIN=PCC 7120 / SAG 25.82 / UTEX 2576;
RX   PubMed=16714404; DOI=10.1104/pp.106.082859;
RA   Awai K., Kakimoto T., Awai C., Kaneko T., Nakamura Y., Takamiya K.,
RA   Wada H., Ohta H.;
RT   "Comparative genomic analysis revealed a gene for
RT   monoglucosyldiacylglycerol synthase, an enzyme for photosynthetic membrane
RT   lipid synthesis in cyanobacteria.";
RL   Plant Physiol. 141:1120-1127(2006).
CC   -!- FUNCTION: Glucosyltransferase involved in the biosynthesis of the non-
CC       bilayer-forming membrane lipid beta-monoglucosyldiacylglycerol which
CC       contributes to regulate the properties and stability of the membrane.
CC       Catalyzes the transfer of a glucosyl residue from UDP-Glc to
CC       diacylglycerol (DAG) acceptor to form the corresponding beta-glucosyl-
CC       DAG (1,2-diacyl-3-O-(beta-D-glucopyranosyl)-sn-glycerol). It can only
CC       use UDP-Glc as sugar donor. Two types of DAG (dipalmitoyl-DAG (DPDAG)
CC       and 1-oleoyl-2-palmitoyl-DAG (OPDAG)) can be used as sugar acceptors,
CC       but OPDAG is preferred. {ECO:0000269|PubMed:16714404}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=a 1,2-diacyl-sn-glycerol + UDP-alpha-D-glucose = a 1,2-diacyl-
CC         3-O-(beta-D-glucopyranosyl)-sn-glycerol + H(+) + UDP;
CC         Xref=Rhea:RHEA:17285, ChEBI:CHEBI:15378, ChEBI:CHEBI:17815,
CC         ChEBI:CHEBI:58223, ChEBI:CHEBI:58885, ChEBI:CHEBI:75799;
CC         EC=2.4.1.336; Evidence={ECO:0000269|PubMed:16714404};
CC   -!- COFACTOR:
CC       Name=Mg(2+); Xref=ChEBI:CHEBI:18420;
CC         Evidence={ECO:0000269|PubMed:16714404};
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Multi-pass membrane
CC       protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the glycosyltransferase 2 family. {ECO:0000305}.
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DR   EMBL; BA000019; BAB76632.1; -; Genomic_DNA.
DR   PIR; AE2422; AE2422.
DR   RefSeq; WP_010999059.1; NZ_RSCN01000018.1.
DR   AlphaFoldDB; Q8YMK0; -.
DR   SMR; Q8YMK0; -.
DR   STRING; 103690.17134071; -.
DR   CAZy; GT2; Glycosyltransferase Family 2.
DR   EnsemblBacteria; BAB76632; BAB76632; BAB76632.
DR   KEGG; ana:all4933; -.
DR   eggNOG; COG1215; Bacteria.
DR   OMA; RNRWAEG; -.
DR   OrthoDB; 724641at2; -.
DR   Proteomes; UP000002483; Chromosome.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0016758; F:hexosyltransferase activity; IDA:UniProtKB.
DR   GO; GO:0000287; F:magnesium ion binding; ISS:UniProtKB.
DR   GO; GO:0006071; P:glycerol metabolic process; IEA:UniProtKB-KW.
DR   GO; GO:0046467; P:membrane lipid biosynthetic process; IDA:UniProtKB.
DR   Gene3D; 3.90.550.10; -; 1.
DR   InterPro; IPR029044; Nucleotide-diphossugar_trans.
DR   SUPFAM; SSF53448; SSF53448; 1.
PE   1: Evidence at protein level;
KW   Carbohydrate metabolism; Glycerol metabolism; Glycosyltransferase;
KW   Lipid biosynthesis; Lipid metabolism; Magnesium; Membrane;
KW   Reference proteome; Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..468
FT                   /note="Beta-monoglucosyldiacylglycerol synthase"
FT                   /id="PRO_0000425269"
FT   TRANSMEM        51..71
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        72..92
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        358..378
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        387..407
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   468 AA;  52828 MW;  CEF120EA6E2530BE CRC64;
     MPANSWPDND SYKELDPLNS LLSDVSTTEE SVVETRDLSL PSRFQGRRGK AALVLTIVWS
     GTIALHLVSW GSIFILGLTT VLGIHALGVV FARPRHYQKE IQGSLPFVSI LVAAKNEEAV
     IAKLAKNLCN LEYPNGQYEV WIIDDNSTDK TPHILAELAK EYDKLKVLRR SAQATGGKSG
     ALNQVLPLTQ GEIIAVFDAD AQVASDMLLH VVPLFQREKV GAVQVRKAIA NAKENFWTKG
     QMAEMSLDIW FQQQRTALGG IGELRGNGQF VRRQALDSCG GWNEETITDD LDLTFRLHLD
     KWDIECLFYP AVQEEGVTTA IALWHQRNRW AEGGYQRYLD YWDLILKNRM GTRKTWDMLM
     FMLTMYILPT AAIPDLLMAL TRHRPPMLGP VTGLSVTMSV VGMFAGLRRI RQEQKFQVHT
     PFVLLLQTMR GTLYMLHWLV VMSSTTARMS FRPKRLKWVK TVHTGTGE