ID   SYN_MYCPN               Reviewed;         455 AA.
AC   P75521;
DT   01-NOV-1997, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-1997, sequence version 1.
DT   03-AUG-2022, entry version 126.
DE   RecName: Full=Asparagine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00534};
DE            EC=6.1.1.22 {ECO:0000255|HAMAP-Rule:MF_00534};
DE   AltName: Full=Asparaginyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00534};
DE            Short=AsnRS {ECO:0000255|HAMAP-Rule:MF_00534};
GN   Name=asnS {ECO:0000255|HAMAP-Rule:MF_00534}; OrderedLocusNames=MPN_252;
GN   ORFNames=MP580;
OS   Mycoplasma pneumoniae (strain ATCC 29342 / M129) (Mycoplasmoides
OS   pneumoniae).
OC   Bacteria; Tenericutes; Mollicutes; Mycoplasmataceae; Mycoplasma.
OX   NCBI_TaxID=272634;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 29342 / M129;
RX   PubMed=8948633; DOI=10.1093/nar/24.22.4420;
RA   Himmelreich R., Hilbert H., Plagens H., Pirkl E., Li B.-C., Herrmann R.;
RT   "Complete sequence analysis of the genome of the bacterium Mycoplasma
RT   pneumoniae.";
RL   Nucleic Acids Res. 24:4420-4449(1996).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + H(+) + L-
CC         asparaginyl-tRNA(Asn); Xref=Rhea:RHEA:11180, Rhea:RHEA-COMP:9659,
CC         Rhea:RHEA-COMP:9674, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC         ChEBI:CHEBI:33019, ChEBI:CHEBI:58048, ChEBI:CHEBI:78442,
CC         ChEBI:CHEBI:78515, ChEBI:CHEBI:456215; EC=6.1.1.22;
CC         Evidence={ECO:0000255|HAMAP-Rule:MF_00534};
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_00534}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00534}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00534}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; U00089; AAB96228.1; -; Genomic_DNA.
DR   PIR; S73906; S73906.
DR   RefSeq; NP_109940.1; NC_000912.1.
DR   RefSeq; WP_010874609.1; NC_000912.1.
DR   AlphaFoldDB; P75521; -.
DR   SMR; P75521; -.
DR   IntAct; P75521; 3.
DR   STRING; 272634.MPN_252; -.
DR   EnsemblBacteria; AAB96228; AAB96228; MPN_252.
DR   KEGG; mpn:MPN_252; -.
DR   PATRIC; fig|272634.6.peg.271; -.
DR   HOGENOM; CLU_004553_2_0_14; -.
DR   OMA; DNMDLAE; -.
DR   BioCyc; MPNE272634:G1GJ3-398-MON; -.
DR   Proteomes; UP000000808; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004816; F:asparagine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR   GO; GO:0006421; P:asparaginyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 2.40.50.140; -; 1.
DR   Gene3D; 3.30.930.10; -; 1.
DR   HAMAP; MF_00534; Asn_tRNA_synth; 1.
DR   InterPro; IPR004364; Aa-tRNA-synt_II.
DR   InterPro; IPR006195; aa-tRNA-synth_II.
DR   InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR   InterPro; IPR004522; Asn-tRNA-ligase.
DR   InterPro; IPR002312; Asp/Asn-tRNA-synth_IIb.
DR   InterPro; IPR012340; NA-bd_OB-fold.
DR   InterPro; IPR004365; NA-bd_OB_tRNA.
DR   Pfam; PF00152; tRNA-synt_2; 1.
DR   Pfam; PF01336; tRNA_anti-codon; 1.
DR   PRINTS; PR01042; TRNASYNTHASP.
DR   SUPFAM; SSF50249; SSF50249; 1.
DR   SUPFAM; SSF55681; SSF55681; 1.
DR   TIGRFAMs; TIGR00457; asnS; 1.
DR   PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..455
FT                   /note="Asparagine--tRNA ligase"
FT                   /id="PRO_0000176433"
SQ   SEQUENCE   455 AA;  51898 MW;  1AB74ADF708E09C9 CRC64;
     MSATAISDLF EKPAQFKNKK IKLTGWLKNK RTSANIIFLE VNDGSTLLNL QAVVKQDQPE
     LFALAESISL ASAVSVSGTV ALTPKSKQPL ELVVKQINVL STARADYPLQ KKEHSLEFFR
     NNAYLRVRAR TYFAIMKVRS LLSQAIFDYF FKNDFVLVHS PILTSNDCEG AGETFELKQG
     KEFFNKTTYL TVSGQFGAEC YAQAFKKVFT FGPTFRAEKS HTSRHLSEFW MIEPEVAFAN
     LKDLIKLIES TVKTVIKQVM QKAKQELDFL EKQFDVKLME RLKQITSTKN FHVLEYTKAL
     EILKTAQASG QANFEVQDFN FGLDLKTEHE RFLCEQHFHN QPVFVINYPK DFKAFYMKQN
     ADGRTVGAVD LLFPQIGEIC GGSEREGNLE KLVERCQAMQ IDTQTLNWYL DMRKWGYFAS
     AGFGLGFDRL LAYICGLENI RDAIPFPRAH GSINY