SYN_PYRAB
ID SYN_PYRAB Reviewed; 434 AA.
AC Q9V251; G8ZG58;
DT 31-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-2000, sequence version 1.
DT 25-MAY-2022, entry version 126.
DE RecName: Full=Asparagine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00534};
DE EC=6.1.1.22 {ECO:0000255|HAMAP-Rule:MF_00534};
DE AltName: Full=Asparaginyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00534};
DE Short=AsnRS {ECO:0000255|HAMAP-Rule:MF_00534};
GN Name=asnS {ECO:0000255|HAMAP-Rule:MF_00534}; OrderedLocusNames=PYRAB02230;
GN ORFNames=PAB2203;
OS Pyrococcus abyssi (strain GE5 / Orsay).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=272844;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=GE5 / Orsay;
RX PubMed=12622808; DOI=10.1046/j.1365-2958.2003.03381.x;
RA Cohen G.N., Barbe V., Flament D., Galperin M., Heilig R., Lecompte O.,
RA Poch O., Prieur D., Querellou J., Ripp R., Thierry J.-C., Van der Oost J.,
RA Weissenbach J., Zivanovic Y., Forterre P.;
RT "An integrated analysis of the genome of the hyperthermophilic archaeon
RT Pyrococcus abyssi.";
RL Mol. Microbiol. 47:1495-1512(2003).
RN [2]
RP GENOME REANNOTATION.
RC STRAIN=GE5 / Orsay;
RX PubMed=22057919; DOI=10.1007/s00284-011-0035-x;
RA Gao J., Wang J.;
RT "Re-annotation of two hyperthermophilic archaea Pyrococcus abyssi GE5 and
RT Pyrococcus furiosus DSM 3638.";
RL Curr. Microbiol. 64:118-129(2012).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + H(+) + L-
CC asparaginyl-tRNA(Asn); Xref=Rhea:RHEA:11180, Rhea:RHEA-COMP:9659,
CC Rhea:RHEA-COMP:9674, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:58048, ChEBI:CHEBI:78442,
CC ChEBI:CHEBI:78515, ChEBI:CHEBI:456215; EC=6.1.1.22;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00534};
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00534}.
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DR EMBL; AJ248283; CAB49147.1; -; Genomic_DNA.
DR EMBL; HE613800; CCE69599.1; -; Genomic_DNA.
DR PIR; D75212; D75212.
DR RefSeq; WP_010867347.1; NC_000868.1.
DR AlphaFoldDB; Q9V251; -.
DR SMR; Q9V251; -.
DR STRING; 272844.PAB2203; -.
DR EnsemblBacteria; CAB49147; CAB49147; PAB2203.
DR GeneID; 1495112; -.
DR KEGG; pab:PAB2203; -.
DR PATRIC; fig|272844.11.peg.239; -.
DR eggNOG; arCOG00407; Archaea.
DR HOGENOM; CLU_004553_2_0_2; -.
DR OMA; DNMDLAE; -.
DR OrthoDB; 24864at2157; -.
DR PhylomeDB; Q9V251; -.
DR Proteomes; UP000000810; Chromosome.
DR Proteomes; UP000009139; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004816; F:asparagine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006421; P:asparaginyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.30.930.10; -; 1.
DR HAMAP; MF_00534; Asn_tRNA_synth; 1.
DR InterPro; IPR004364; Aa-tRNA-synt_II.
DR InterPro; IPR006195; aa-tRNA-synth_II.
DR InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR InterPro; IPR004522; Asn-tRNA-ligase.
DR InterPro; IPR002312; Asp/Asn-tRNA-synth_IIb.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR Pfam; PF00152; tRNA-synt_2; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR PRINTS; PR01042; TRNASYNTHASP.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF55681; SSF55681; 1.
DR TIGRFAMs; TIGR00457; asnS; 1.
DR PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis.
FT CHAIN 1..434
FT /note="Asparagine--tRNA ligase"
FT /id="PRO_0000176485"
SQ SEQUENCE 434 AA; 50242 MW; 9A08BBCE2458A6A3 CRC64;
MIEKTYCQDI KPELDGKRVK LAGWVYSNMR VGKKIFLWIR DSTGIIQTVI AKNVVGEDVF
ETAKKLGRES SVIVEGIVKA DERAPGGAEV HVEKLKVIQA VSEFPIPENP EQASPELLLD
YRHLHIRSPK VSAIMRVKET LIMAAREWLL REGWHEVFPP ILVTGAVEGG ATLFKLKYFD
KVAYLSQSAQ LYLEAAIFGL EKVWSLTPSF RAEKSRTRRH LTEFWHLELE AAWMDLWDIM
KVEEELVSYM VQRTLELRKK EIEMFRDDLT TLKNTEPPFP RISYDEAIEI LQSKGVKIEW
GDDMGADEER VLTQEFDRPF FVYGYPKHIK AFYMKEDPND PRKVLAADML APEGYGEIIG
GSEREDNYDK LVQRIKEEGM DPKDYEWYLD LRKYGSVPHS GFGLGVERLV AWVLKLDHIR
WATLFPRTPA RIYP
