SYN_PYRHO
ID SYN_PYRHO Reviewed; 434 AA.
AC O57980;
DT 31-JAN-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-AUG-1998, sequence version 1.
DT 25-MAY-2022, entry version 137.
DE RecName: Full=Asparagine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00534};
DE EC=6.1.1.22 {ECO:0000255|HAMAP-Rule:MF_00534};
DE AltName: Full=Asparaginyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00534};
DE Short=AsnRS {ECO:0000255|HAMAP-Rule:MF_00534};
GN Name=asnS {ECO:0000255|HAMAP-Rule:MF_00534}; OrderedLocusNames=PH0241;
OS Pyrococcus horikoshii (strain ATCC 700860 / DSM 12428 / JCM 9974 / NBRC
OS 100139 / OT-3).
OC Archaea; Euryarchaeota; Thermococci; Thermococcales; Thermococcaceae;
OC Pyrococcus.
OX NCBI_TaxID=70601;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700860 / DSM 12428 / JCM 9974 / NBRC 100139 / OT-3;
RX PubMed=9679194; DOI=10.1093/dnares/5.2.55;
RA Kawarabayasi Y., Sawada M., Horikawa H., Haikawa Y., Hino Y., Yamamoto S.,
RA Sekine M., Baba S., Kosugi H., Hosoyama A., Nagai Y., Sakai M., Ogura K.,
RA Otsuka R., Nakazawa H., Takamiya M., Ohfuku Y., Funahashi T., Tanaka T.,
RA Kudoh Y., Yamazaki J., Kushida N., Oguchi A., Aoki K., Yoshizawa T.,
RA Nakamura Y., Robb F.T., Horikoshi K., Masuchi Y., Shizuya H., Kikuchi H.;
RT "Complete sequence and gene organization of the genome of a hyper-
RT thermophilic archaebacterium, Pyrococcus horikoshii OT3.";
RL DNA Res. 5:55-76(1998).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-asparagine + tRNA(Asn) = AMP + diphosphate + H(+) + L-
CC asparaginyl-tRNA(Asn); Xref=Rhea:RHEA:11180, Rhea:RHEA-COMP:9659,
CC Rhea:RHEA-COMP:9674, ChEBI:CHEBI:15378, ChEBI:CHEBI:30616,
CC ChEBI:CHEBI:33019, ChEBI:CHEBI:58048, ChEBI:CHEBI:78442,
CC ChEBI:CHEBI:78515, ChEBI:CHEBI:456215; EC=6.1.1.22;
CC Evidence={ECO:0000255|HAMAP-Rule:MF_00534};
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00534}.
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DR EMBL; BA000001; BAA29313.1; -; Genomic_DNA.
DR PIR; B71248; B71248.
DR RefSeq; WP_010884344.1; NC_000961.1.
DR PDB; 1X54; X-ray; 1.45 A; A=1-434.
DR PDB; 1X55; X-ray; 1.80 A; A=1-434.
DR PDB; 1X56; X-ray; 1.98 A; A=1-434.
DR PDBsum; 1X54; -.
DR PDBsum; 1X55; -.
DR PDBsum; 1X56; -.
DR AlphaFoldDB; O57980; -.
DR SMR; O57980; -.
DR STRING; 70601.3256630; -.
DR EnsemblBacteria; BAA29313; BAA29313; BAA29313.
DR GeneID; 1444131; -.
DR KEGG; pho:PH0241; -.
DR eggNOG; arCOG00407; Archaea.
DR OMA; DNMDLAE; -.
DR OrthoDB; 24864at2157; -.
DR BRENDA; 6.1.1.22; 5244.
DR EvolutionaryTrace; O57980; -.
DR Proteomes; UP000000752; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004816; F:asparagine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0003676; F:nucleic acid binding; IEA:InterPro.
DR GO; GO:0006421; P:asparaginyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR Gene3D; 2.40.50.140; -; 1.
DR Gene3D; 3.30.930.10; -; 1.
DR HAMAP; MF_00534; Asn_tRNA_synth; 1.
DR InterPro; IPR004364; Aa-tRNA-synt_II.
DR InterPro; IPR006195; aa-tRNA-synth_II.
DR InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR InterPro; IPR004522; Asn-tRNA-ligase.
DR InterPro; IPR002312; Asp/Asn-tRNA-synth_IIb.
DR InterPro; IPR012340; NA-bd_OB-fold.
DR InterPro; IPR004365; NA-bd_OB_tRNA.
DR Pfam; PF00152; tRNA-synt_2; 1.
DR Pfam; PF01336; tRNA_anti-codon; 1.
DR PRINTS; PR01042; TRNASYNTHASP.
DR SUPFAM; SSF50249; SSF50249; 1.
DR SUPFAM; SSF55681; SSF55681; 1.
DR TIGRFAMs; TIGR00457; asnS; 1.
DR PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis.
FT CHAIN 1..434
FT /note="Asparagine--tRNA ligase"
FT /id="PRO_0000176487"
FT HELIX 7..9
FT /evidence="ECO:0007829|PDB:1X54"
FT HELIX 12..14
FT /evidence="ECO:0007829|PDB:1X54"
FT STRAND 18..31
FT /evidence="ECO:0007829|PDB:1X54"
FT STRAND 34..41
FT /evidence="ECO:0007829|PDB:1X54"
FT STRAND 44..50
FT /evidence="ECO:0007829|PDB:1X54"
FT HELIX 52..55
FT /evidence="ECO:0007829|PDB:1X54"
FT HELIX 57..64
FT /evidence="ECO:0007829|PDB:1X54"
FT STRAND 71..80
FT /evidence="ECO:0007829|PDB:1X54"
FT HELIX 85..87
FT /evidence="ECO:0007829|PDB:1X54"
FT STRAND 88..99
FT /evidence="ECO:0007829|PDB:1X54"
FT HELIX 110..112
FT /evidence="ECO:0007829|PDB:1X54"
FT HELIX 115..120
FT /evidence="ECO:0007829|PDB:1X54"
FT HELIX 122..125
FT /evidence="ECO:0007829|PDB:1X54"
FT HELIX 129..151
FT /evidence="ECO:0007829|PDB:1X54"
FT STRAND 161..164
FT /evidence="ECO:0007829|PDB:1X54"
FT HELIX 170..172
FT /evidence="ECO:0007829|PDB:1X54"
FT STRAND 175..178
FT /evidence="ECO:0007829|PDB:1X54"
FT STRAND 181..185
FT /evidence="ECO:0007829|PDB:1X54"
FT HELIX 190..200
FT /evidence="ECO:0007829|PDB:1X54"
FT STRAND 201..210
FT /evidence="ECO:0007829|PDB:1X54"
FT STRAND 222..232
FT /evidence="ECO:0007829|PDB:1X54"
FT HELIX 236..257
FT /evidence="ECO:0007829|PDB:1X54"
FT HELIX 259..263
FT /evidence="ECO:0007829|PDB:1X54"
FT HELIX 270..273
FT /evidence="ECO:0007829|PDB:1X54"
FT STRAND 281..283
FT /evidence="ECO:0007829|PDB:1X54"
FT HELIX 284..293
FT /evidence="ECO:0007829|PDB:1X54"
FT HELIX 306..313
FT /evidence="ECO:0007829|PDB:1X54"
FT STRAND 320..326
FT /evidence="ECO:0007829|PDB:1X54"
FT HELIX 327..329
FT /evidence="ECO:0007829|PDB:1X54"
FT STRAND 343..351
FT /evidence="ECO:0007829|PDB:1X54"
FT TURN 352..355
FT /evidence="ECO:0007829|PDB:1X54"
FT STRAND 356..364
FT /evidence="ECO:0007829|PDB:1X54"
FT HELIX 368..377
FT /evidence="ECO:0007829|PDB:1X54"
FT HELIX 382..385
FT /evidence="ECO:0007829|PDB:1X54"
FT HELIX 386..390
FT /evidence="ECO:0007829|PDB:1X54"
FT TURN 391..393
FT /evidence="ECO:0007829|PDB:1X54"
FT STRAND 399..405
FT /evidence="ECO:0007829|PDB:1X54"
FT HELIX 406..413
FT /evidence="ECO:0007829|PDB:1X54"
FT HELIX 419..422
FT /evidence="ECO:0007829|PDB:1X54"
FT STRAND 423..425
FT /evidence="ECO:0007829|PDB:1X54"
SQ SEQUENCE 434 AA; 50160 MW; 458F1447BC0CD1DF CRC64;
MIEKVYCQEV KPELDGKKVR LAGWVYTNMR VGKKIFLWIR DSTGIVQAVV AKNVVGEETF
EKAKKLGRES SVIVEGIVKA DERAPGGAEV HVEKLEVIQA VSEFPIPENP EQASPELLLD
YRHLHIRTPK ASAIMKVKET LIMAAREWLL KDGWHEVFPP ILVTGAVEGG ATLFKLKYFD
KYAYLSQSAQ LYLEAAIFGL EKVWSLTPSF RAEKSRTRRH LTEFWHLELE AAWMDLWDIM
KVEEELVSYM VQRTLELRKK EIEMFRDDLT TLKNTEPPFP RISYDEAIDI LQSKGVNVEW
GDDLGADEER VLTEEFDRPF FVYGYPKHIK AFYMKEDPND PRKVLASDML APEGYGEIIG
GSQREDDYDK LLNRILEEGM DPKDYEWYLD LRRYGSVPHS GFGLGVERLV AWVLKLDHIR
WAALFPRTPA RLYP
