ID   SYP81_ARATH             Reviewed;         310 AA.
AC   P59277; Q84JQ3; Q9C8I2;
DT   12-FEB-2003, integrated into UniProtKB/Swiss-Prot.
DT   29-MAY-2007, sequence version 2.
DT   03-AUG-2022, entry version 113.
DE   RecName: Full=Syntaxin-81;
DE            Short=AtSYP81;
GN   Name=SYP81; OrderedLocusNames=At1g51740; ORFNames=F19C24.5;
OS   Arabidopsis thaliana (Mouse-ear cress).
OC   Eukaryota; Viridiplantae; Streptophyta; Embryophyta; Tracheophyta;
OC   Spermatophyta; Magnoliopsida; eudicotyledons; Gunneridae; Pentapetalae;
OC   rosids; malvids; Brassicales; Brassicaceae; Camelineae; Arabidopsis.
OX   NCBI_TaxID=3702;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=11130712; DOI=10.1038/35048500;
RA   Theologis A., Ecker J.R., Palm C.J., Federspiel N.A., Kaul S., White O.,
RA   Alonso J., Altafi H., Araujo R., Bowman C.L., Brooks S.Y., Buehler E.,
RA   Chan A., Chao Q., Chen H., Cheuk R.F., Chin C.W., Chung M.K., Conn L.,
RA   Conway A.B., Conway A.R., Creasy T.H., Dewar K., Dunn P., Etgu P.,
RA   Feldblyum T.V., Feng J.-D., Fong B., Fujii C.Y., Gill J.E., Goldsmith A.D.,
RA   Haas B., Hansen N.F., Hughes B., Huizar L., Hunter J.L., Jenkins J.,
RA   Johnson-Hopson C., Khan S., Khaykin E., Kim C.J., Koo H.L.,
RA   Kremenetskaia I., Kurtz D.B., Kwan A., Lam B., Langin-Hooper S., Lee A.,
RA   Lee J.M., Lenz C.A., Li J.H., Li Y.-P., Lin X., Liu S.X., Liu Z.A.,
RA   Luros J.S., Maiti R., Marziali A., Militscher J., Miranda M., Nguyen M.,
RA   Nierman W.C., Osborne B.I., Pai G., Peterson J., Pham P.K., Rizzo M.,
RA   Rooney T., Rowley D., Sakano H., Salzberg S.L., Schwartz J.R., Shinn P.,
RA   Southwick A.M., Sun H., Tallon L.J., Tambunga G., Toriumi M.J., Town C.D.,
RA   Utterback T., Van Aken S., Vaysberg M., Vysotskaia V.S., Walker M., Wu D.,
RA   Yu G., Fraser C.M., Venter J.C., Davis R.W.;
RT   "Sequence and analysis of chromosome 1 of the plant Arabidopsis thaliana.";
RL   Nature 408:816-820(2000).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=cv. Columbia;
RX   PubMed=27862469; DOI=10.1111/tpj.13415;
RA   Cheng C.Y., Krishnakumar V., Chan A.P., Thibaud-Nissen F., Schobel S.,
RA   Town C.D.;
RT   "Araport11: a complete reannotation of the Arabidopsis thaliana reference
RT   genome.";
RL   Plant J. 89:789-804(2017).
RN   [3]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   STRAIN=cv. Columbia;
RX   PubMed=14593172; DOI=10.1126/science.1088305;
RA   Yamada K., Lim J., Dale J.M., Chen H., Shinn P., Palm C.J., Southwick A.M.,
RA   Wu H.C., Kim C.J., Nguyen M., Pham P.K., Cheuk R.F., Karlin-Newmann G.,
RA   Liu S.X., Lam B., Sakano H., Wu T., Yu G., Miranda M., Quach H.L.,
RA   Tripp M., Chang C.H., Lee J.M., Toriumi M.J., Chan M.M., Tang C.C.,
RA   Onodera C.S., Deng J.M., Akiyama K., Ansari Y., Arakawa T., Banh J.,
RA   Banno F., Bowser L., Brooks S.Y., Carninci P., Chao Q., Choy N., Enju A.,
RA   Goldsmith A.D., Gurjal M., Hansen N.F., Hayashizaki Y., Johnson-Hopson C.,
RA   Hsuan V.W., Iida K., Karnes M., Khan S., Koesema E., Ishida J., Jiang P.X.,
RA   Jones T., Kawai J., Kamiya A., Meyers C., Nakajima M., Narusaka M.,
RA   Seki M., Sakurai T., Satou M., Tamse R., Vaysberg M., Wallender E.K.,
RA   Wong C., Yamamura Y., Yuan S., Shinozaki K., Davis R.W., Theologis A.,
RA   Ecker J.R.;
RT   "Empirical analysis of transcriptional activity in the Arabidopsis
RT   genome.";
RL   Science 302:842-846(2003).
RN   [4]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RA   Brover V.V., Troukhan M.E., Alexandrov N.A., Lu Y.-P., Flavell R.B.,
RA   Feldmann K.A.;
RT   "Full-length cDNA from Arabidopsis thaliana.";
RL   Submitted (MAR-2002) to the EMBL/GenBank/DDBJ databases.
RN   [5]
RP   INTERACTION WITH MAG2.
RX   PubMed=17194767; DOI=10.1105/tpc.106.046151;
RA   Li L., Shimada T., Takahashi H., Ueda H., Fukao Y., Kondo M., Nishimura M.,
RA   Hara-Nishimura I.;
RT   "MAIGO2 is involved in exit of seed storage proteins from the endoplasmic
RT   reticulum in Arabidopsis thaliana.";
RL   Plant Cell 18:3535-3547(2006).
CC   -!- FUNCTION: Vesicle trafficking protein that functions in the secretory
CC       pathway. {ECO:0000305}.
CC   -!- SUBUNIT: Part of the t-SNARE complex. Interacts with MAG2
CC       (PubMed:17194767). {ECO:0000269|PubMed:17194767, ECO:0000305}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000255}; Single-pass type IV
CC       membrane protein {ECO:0000255}.
CC   -!- SIMILARITY: Belongs to the syntaxin family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=AAG50888.1; Type=Erroneous gene model prediction; Evidence={ECO:0000305};
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DR   EMBL; AC025294; AAG50888.1; ALT_SEQ; Genomic_DNA.
DR   EMBL; CP002684; AEE32709.1; -; Genomic_DNA.
DR   EMBL; BT002917; AAO22733.1; -; mRNA.
DR   EMBL; BT004352; AAO42346.1; -; mRNA.
DR   EMBL; AY086569; AAM63632.1; -; mRNA.
DR   RefSeq; NP_564597.1; NM_104052.3.
DR   AlphaFoldDB; P59277; -.
DR   SMR; P59277; -.
DR   IntAct; P59277; 4.
DR   STRING; 3702.AT1G51740.1; -.
DR   PaxDb; P59277; -.
DR   PRIDE; P59277; -.
DR   ProteomicsDB; 233067; -.
DR   EnsemblPlants; AT1G51740.1; AT1G51740.1; AT1G51740.
DR   GeneID; 841599; -.
DR   Gramene; AT1G51740.1; AT1G51740.1; AT1G51740.
DR   KEGG; ath:AT1G51740; -.
DR   Araport; AT1G51740; -.
DR   TAIR; locus:2017632; AT1G51740.
DR   eggNOG; KOG3894; Eukaryota.
DR   HOGENOM; CLU_054329_0_0_1; -.
DR   OMA; WEESRVE; -.
DR   OrthoDB; 1141750at2759; -.
DR   PhylomeDB; P59277; -.
DR   PRO; PR:P59277; -.
DR   Proteomes; UP000006548; Chromosome 1.
DR   ExpressionAtlas; P59277; baseline and differential.
DR   Genevisible; P59277; AT.
DR   GO; GO:0005783; C:endoplasmic reticulum; IBA:GO_Central.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0031201; C:SNARE complex; IBA:GO_Central.
DR   GO; GO:0015031; P:protein transport; IEA:UniProtKB-KW.
DR   GO; GO:0006890; P:retrograde vesicle-mediated transport, Golgi to endoplasmic reticulum; IBA:GO_Central.
DR   InterPro; IPR010989; SNARE.
DR   InterPro; IPR019529; Syntaxin-18_N.
DR   Pfam; PF10496; Syntaxin-18_N; 1.
DR   SUPFAM; SSF47661; SSF47661; 1.
PE   1: Evidence at protein level;
KW   Coiled coil; Membrane; Protein transport; Reference proteome;
KW   Transmembrane; Transmembrane helix; Transport.
FT   CHAIN           1..310
FT                   /note="Syntaxin-81"
FT                   /id="PRO_0000210267"
FT   TOPO_DOM        1..289
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        290..310
FT                   /note="Helical; Anchor for type IV membrane protein"
FT                   /evidence="ECO:0000255"
FT   COILED          77..114
FT                   /evidence="ECO:0000255"
FT   CONFLICT        17
FT                   /note="N -> K (in Ref. 4; AAM63632)"
FT                   /evidence="ECO:0000305"
SQ   SEQUENCE   310 AA;  35573 MW;  7D2BE2B8C909A1A6 CRC64;
     MSRFRDRTED FKDSVRNSAV SIGYNESKVA STMASFIIHK PKERSPFTKA AFKTLDSIKE
     LELFMLKHRK DYVDLHRTTE QEKDSIEQEV AAFIKACKEQ IDILINSIRN EEANSKGWLG
     LPADNFNADS IAHKHGVVLI LSEKLHSVTA QFDQLRATRF QDIINRAMPR RKPKRVIKEA
     TPINTTLGNS ESIEPDEIQA QPRRLQQQQL LDDETQALQV ELSNLLDGAR QTETKMVEMS
     ALNHLMATHV LQQAQQIEFL YDQAVEATKN VELGNKELSQ AIQRNSSSRT FLLLFFFVLT
     FSVLFLDWYS