SYPL1_MOUSE
ID SYPL1_MOUSE Reviewed; 261 AA.
AC O09117; Q8BQI8; Q8BQJ1; Q8C749;
DT 10-OCT-2003, integrated into UniProtKB/Swiss-Prot.
DT 10-OCT-2003, sequence version 2.
DT 03-AUG-2022, entry version 158.
DE RecName: Full=Synaptophysin-like protein 1;
DE AltName: Full=Pantophysin;
GN Name=Sypl1; Synonyms=Pphn, Sypl;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA] (ISOFORM 1), SUBCELLULAR LOCATION, AND
RP TISSUE SPECIFICITY.
RC STRAIN=129/SvJ;
RX PubMed=8707851; DOI=10.1083/jcb.134.3.731;
RA Haass N.K., Kartenbeck J., Leube R.E.;
RT "Pantophysin is a ubiquitously expressed synaptophysin homologue and
RT defines constitutive transport vesicles.";
RL J. Cell Biol. 134:731-746(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
RC STRAIN=C57BL/6J; TISSUE=Stomach;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 1).
RC TISSUE=Testis;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [4]
RP PROTEIN SEQUENCE OF 58-66, AND IDENTIFICATION BY MASS SPECTROMETRY.
RC STRAIN=C57BL/6J; TISSUE=Brain;
RA Lubec G., Kang S.U.;
RL Submitted (APR-2007) to UniProtKB.
RN [5]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-72.
RC TISSUE=Myoblast;
RX PubMed=19656770; DOI=10.1074/mcp.m900195-mcp200;
RA Gundry R.L., Raginski K., Tarasova Y., Tchernyshyov I., Bausch-Fluck D.,
RA Elliott S.T., Boheler K.R., Van Eyk J.E., Wollscheid B.;
RT "The mouse C2C12 myoblast cell surface N-linked glycoproteome:
RT identification, glycosite occupancy, and membrane orientation.";
RL Mol. Cell. Proteomics 8:2555-2569(2009).
RN [6]
RP GLYCOSYLATION [LARGE SCALE ANALYSIS] AT ASN-72 AND ASN-95.
RX PubMed=19349973; DOI=10.1038/nbt.1532;
RA Wollscheid B., Bausch-Fluck D., Henderson C., O'Brien R., Bibel M.,
RA Schiess R., Aebersold R., Watts J.D.;
RT "Mass-spectrometric identification and relative quantification of N-linked
RT cell surface glycoproteins.";
RL Nat. Biotechnol. 27:378-386(2009).
RN [7]
RP IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
RC TISSUE=Brown adipose tissue, Heart, Kidney, Liver, Lung, Pancreas, and
RC Testis;
RX PubMed=21183079; DOI=10.1016/j.cell.2010.12.001;
RA Huttlin E.L., Jedrychowski M.P., Elias J.E., Goswami T., Rad R.,
RA Beausoleil S.A., Villen J., Haas W., Sowa M.E., Gygi S.P.;
RT "A tissue-specific atlas of mouse protein phosphorylation and expression.";
RL Cell 143:1174-1189(2010).
CC -!- SUBCELLULAR LOCATION: Cytoplasmic vesicle membrane
CC {ECO:0000269|PubMed:8707851}; Multi-pass membrane protein
CC {ECO:0000269|PubMed:8707851}. Melanosome {ECO:0000250}.
CC Note=Cytoplasmic transport vesicles.
CC -!- ALTERNATIVE PRODUCTS:
CC Event=Alternative splicing; Named isoforms=2;
CC Name=1;
CC IsoId=O09117-1; Sequence=Displayed;
CC Name=2;
CC IsoId=O09117-2; Sequence=VSP_008558;
CC -!- TISSUE SPECIFICITY: Ubiquitously expressed.
CC {ECO:0000269|PubMed:8707851}.
CC -!- SIMILARITY: Belongs to the synaptophysin/synaptobrevin family.
CC {ECO:0000305}.
CC -!- SEQUENCE CAUTION:
CC Sequence=BAC33808.1; Type=Erroneous initiation; Evidence={ECO:0000305};
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; AF081501; AAB39891.1; -; Genomic_DNA.
DR EMBL; AK049545; BAC33803.1; -; mRNA.
DR EMBL; AK049556; BAC33808.1; ALT_INIT; mRNA.
DR EMBL; AK052556; BAC35036.1; -; mRNA.
DR EMBL; BC060971; AAH60971.1; -; mRNA.
DR CCDS; CCDS25872.1; -. [O09117-1]
DR CCDS; CCDS25873.1; -. [O09117-2]
DR RefSeq; NP_038663.2; NM_013635.3. [O09117-1]
DR RefSeq; NP_942003.1; NM_198710.3. [O09117-2]
DR AlphaFoldDB; O09117; -.
DR IntAct; O09117; 1.
DR MINT; O09117; -.
DR STRING; 10090.ENSMUSP00000075990; -.
DR GlyConnect; 2749; 9 N-Linked glycans (1 site).
DR GlyGen; O09117; 3 sites, 9 N-linked glycans (1 site).
DR iPTMnet; O09117; -.
DR PhosphoSitePlus; O09117; -.
DR EPD; O09117; -.
DR jPOST; O09117; -.
DR MaxQB; O09117; -.
DR PaxDb; O09117; -.
DR PeptideAtlas; O09117; -.
DR PRIDE; O09117; -.
DR ProteomicsDB; 254507; -. [O09117-1]
DR ProteomicsDB; 254508; -. [O09117-2]
DR Antibodypedia; 2819; 159 antibodies from 25 providers.
DR DNASU; 19027; -.
DR Ensembl; ENSMUST00000020885; ENSMUSP00000020885; ENSMUSG00000020570. [O09117-2]
DR Ensembl; ENSMUST00000076698; ENSMUSP00000075990; ENSMUSG00000020570. [O09117-1]
DR GeneID; 19027; -.
DR KEGG; mmu:19027; -.
DR UCSC; uc007nih.2; mouse. [O09117-1]
DR CTD; 19027; -.
DR MGI; MGI:108081; Sypl.
DR VEuPathDB; HostDB:ENSMUSG00000020570; -.
DR eggNOG; ENOG502RY2D; Eukaryota.
DR GeneTree; ENSGT01030000234637; -.
DR HOGENOM; CLU_064642_1_0_1; -.
DR InParanoid; O09117; -.
DR OMA; YNVPDCK; -.
DR OrthoDB; 1446480at2759; -.
DR PhylomeDB; O09117; -.
DR TreeFam; TF315804; -.
DR BioGRID-ORCS; 19027; 3 hits in 72 CRISPR screens.
DR ChiTaRS; Sypl; mouse.
DR PRO; PR:O09117; -.
DR Proteomes; UP000000589; Chromosome 12.
DR RNAct; O09117; protein.
DR Bgee; ENSMUSG00000020570; Expressed in spermatid and 257 other tissues.
DR ExpressionAtlas; O09117; baseline and differential.
DR Genevisible; O09117; MM.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0042470; C:melanosome; IEA:UniProtKB-SubCell.
DR GO; GO:0016020; C:membrane; IDA:MGI.
DR GO; GO:0030141; C:secretory granule; IDA:MGI.
DR GO; GO:0030672; C:synaptic vesicle membrane; IBA:GO_Central.
DR InterPro; IPR008253; Marvel.
DR InterPro; IPR001285; Synaptophysin/porin.
DR PANTHER; PTHR10306; PTHR10306; 1.
DR Pfam; PF01284; MARVEL; 1.
DR PRINTS; PR00220; SYNAPTOPHYSN.
DR PROSITE; PS51225; MARVEL; 1.
PE 1: Evidence at protein level;
KW Alternative splicing; Cytoplasmic vesicle; Direct protein sequencing;
KW Glycoprotein; Membrane; Reference proteome; Transmembrane;
KW Transmembrane helix.
FT CHAIN 1..261
FT /note="Synaptophysin-like protein 1"
FT /id="PRO_0000179165"
FT TOPO_DOM 1..33
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 34..54
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 55..117
FT /note="Vesicular"
FT /evidence="ECO:0000255"
FT TRANSMEM 118..138
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 139..151
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT TRANSMEM 152..172
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 173..214
FT /note="Vesicular"
FT /evidence="ECO:0000255"
FT TRANSMEM 215..235
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TOPO_DOM 236..261
FT /note="Cytoplasmic"
FT /evidence="ECO:0000255"
FT DOMAIN 28..239
FT /note="MARVEL"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00581"
FT REGION 241..261
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT CARBOHYD 72
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:19349973,
FT ECO:0000269|PubMed:19656770"
FT CARBOHYD 95
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000269|PubMed:19349973"
FT CARBOHYD 214
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT VAR_SEQ 1..18
FT /note="Missing (in isoform 2)"
FT /evidence="ECO:0000303|PubMed:16141072"
FT /id="VSP_008558"
FT CONFLICT 97
FT /note="S -> N (in Ref. 1; AAB39891)"
FT /evidence="ECO:0000305"
FT CONFLICT 202
FT /note="C -> R (in Ref. 2; BAC35036)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 261 AA; 28899 MW; 5140C6B432B9E282 CRC64;
MASKANMVRQ RFSRLSQRMS AFQINLNPLK EPLGFIKILE WFASIFAFAT CGGFKGKTEI
QVNCPKVGVN KNQTVTATFG YPFRLNQASF HTPPNVSVCD VNWEKHVLIG DYSSSAQFYV
TFAVFVFLYC IAALLLYVGY TNLYRDSRKL PMIDFIVTLV ATFLWLVSSS AWAKALTDIK
VATGHRIVEE LEICNPESGV SCYFVSVTSM GSLNVSVIFG FLNMILWGGN AWFVYKETSL
HSPSNTSASH SQGGGPPTSG M
