ID   SYPM_RAT                Reviewed;         475 AA.
AC   Q5M7W7;
DT   30-AUG-2005, integrated into UniProtKB/Swiss-Prot.
DT   01-FEB-2005, sequence version 1.
DT   03-AUG-2022, entry version 111.
DE   RecName: Full=Probable proline--tRNA ligase, mitochondrial;
DE            EC=6.1.1.15;
DE   AltName: Full=Prolyl-tRNA synthetase;
DE            Short=ProRS;
DE   Flags: Precursor;
GN   Name=Pars2;
OS   Rattus norvegicus (Rat).
OC   Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC   Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC   Murinae; Rattus.
OX   NCBI_TaxID=10116;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC   TISSUE=Kidney;
RX   PubMed=15489334; DOI=10.1101/gr.2596504;
RG   The MGC Project Team;
RT   "The status, quality, and expansion of the NIH full-length cDNA project:
RT   the Mammalian Gene Collection (MGC).";
RL   Genome Res. 14:2121-2127(2004).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-
CC         tRNA(Pro); Xref=Rhea:RHEA:14305, Rhea:RHEA-COMP:9700, Rhea:RHEA-
CC         COMP:9702, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:60039,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78532, ChEBI:CHEBI:456215;
CC         EC=6.1.1.15;
CC   -!- SUBCELLULAR LOCATION: Mitochondrion matrix {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       {ECO:0000305}.
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DR   EMBL; BC088403; AAH88403.1; -; mRNA.
DR   RefSeq; NP_001014086.1; NM_001014064.1.
DR   RefSeq; XP_006238575.1; XM_006238513.3.
DR   RefSeq; XP_017448872.1; XM_017593383.1.
DR   AlphaFoldDB; Q5M7W7; -.
DR   SMR; Q5M7W7; -.
DR   STRING; 10116.ENSRNOP00000054281; -.
DR   iPTMnet; Q5M7W7; -.
DR   PhosphoSitePlus; Q5M7W7; -.
DR   PaxDb; Q5M7W7; -.
DR   PRIDE; Q5M7W7; -.
DR   Ensembl; ENSRNOT00000057471; ENSRNOP00000054281; ENSRNOG00000007327.
DR   GeneID; 313429; -.
DR   KEGG; rno:313429; -.
DR   CTD; 25973; -.
DR   RGD; 1305345; Pars2.
DR   eggNOG; KOG2324; Eukaryota.
DR   GeneTree; ENSGT00390000010922; -.
DR   HOGENOM; CLU_016739_4_1_1; -.
DR   InParanoid; Q5M7W7; -.
DR   OMA; NCDYAAN; -.
DR   OrthoDB; 409116at2759; -.
DR   PhylomeDB; Q5M7W7; -.
DR   PRO; PR:Q5M7W7; -.
DR   Proteomes; UP000002494; Chromosome 5.
DR   Bgee; ENSRNOG00000007327; Expressed in skeletal muscle tissue and 20 other tissues.
DR   GO; GO:0005759; C:mitochondrial matrix; IEA:UniProtKB-SubCell.
DR   GO; GO:0005739; C:mitochondrion; IBA:GO_Central.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR   GO; GO:0004827; F:proline-tRNA ligase activity; IBA:GO_Central.
DR   GO; GO:0006433; P:prolyl-tRNA aminoacylation; IBA:GO_Central.
DR   CDD; cd00861; ProRS_anticodon_short; 1.
DR   CDD; cd00779; ProRS_core_prok; 1.
DR   Gene3D; 3.30.930.10; -; 1.
DR   Gene3D; 3.40.50.800; -; 1.
DR   InterPro; IPR002314; aa-tRNA-synt_IIb.
DR   InterPro; IPR006195; aa-tRNA-synth_II.
DR   InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR   InterPro; IPR004154; Anticodon-bd.
DR   InterPro; IPR036621; Anticodon-bd_dom_sf.
DR   InterPro; IPR002316; Pro-tRNA-ligase_IIa.
DR   InterPro; IPR044140; ProRS_anticodon_short.
DR   InterPro; IPR033730; ProRS_core_prok.
DR   Pfam; PF03129; HGTP_anticodon; 1.
DR   Pfam; PF00587; tRNA-synt_2b; 1.
DR   PRINTS; PR01046; TRNASYNTHPRO.
DR   SUPFAM; SSF55681; SSF55681; 1.
DR   PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE   2: Evidence at transcript level;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Ligase; Mitochondrion;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome;
KW   Transit peptide.
FT   TRANSIT         1..29
FT                   /note="Mitochondrion"
FT                   /evidence="ECO:0000255"
FT   CHAIN           30..475
FT                   /note="Probable proline--tRNA ligase, mitochondrial"
FT                   /id="PRO_0000035820"
SQ   SEQUENCE   475 AA;  53333 MW;  DC56602694F0DCC1 CRC64;
     MEGLLTRCRT LSALATCSLR HSRCIVRKCY HCAPGRGQRL VVSRMFQPQN LREDQVLSLE
     GRASDLTCKS QRLMLQVGLI LPASPGCYHL MPYTVRAVEK LVRVIDQEMQ AIGGQKINMP
     SLSPAELWRA TGRWDLMGRE LLRLKDRHGK EYCLGPTHEE AVTALVASQK KLSYKQLPLL
     LYQVTRKFRD EPRPRFGLLR GREFYMKDMY TFDSSSEAAQ ETYSLVCDAY CRLFDRLGLR
     WMKARADVGS IGGTMSHEFQ LPVDIGEDRL VVCPSCHFSA NTEIVDLSQK ICPDCQGPLT
     ETKGIEVGHT FYLGTKYSSI FNAHFTNAHG ESLLAEMGCY GLGVTRILAA AIEVLSTEDC
     IRWPSLLAPY QVCIIPPKKG SKEAAATEIV ERLYDDVTEA LPQLRGEVLL DDRTHLTIGN
     RLKDANKLGY PFVIIASKRA LEDPAHFEVW SQNTGEVVFL TKEGVMELLT GVHVV