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SYP_ENTFA
ID   SYP_ENTFA               Reviewed;         572 AA.
AC   Q831W7;
DT   05-SEP-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-JUN-2003, sequence version 1.
DT   03-AUG-2022, entry version 119.
DE   RecName: Full=Proline--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_01569};
DE            EC=6.1.1.15 {ECO:0000255|HAMAP-Rule:MF_01569};
DE   AltName: Full=Prolyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_01569};
DE            Short=ProRS {ECO:0000255|HAMAP-Rule:MF_01569};
GN   Name=proS {ECO:0000255|HAMAP-Rule:MF_01569}; OrderedLocusNames=EF_2379;
OS   Enterococcus faecalis (strain ATCC 700802 / V583).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Enterococcaceae;
OC   Enterococcus.
OX   NCBI_TaxID=226185;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700802 / V583;
RX   PubMed=12663927; DOI=10.1126/science.1080613;
RA   Paulsen I.T., Banerjei L., Myers G.S.A., Nelson K.E., Seshadri R.,
RA   Read T.D., Fouts D.E., Eisen J.A., Gill S.R., Heidelberg J.F., Tettelin H.,
RA   Dodson R.J., Umayam L.A., Brinkac L.M., Beanan M.J., Daugherty S.C.,
RA   DeBoy R.T., Durkin S.A., Kolonay J.F., Madupu R., Nelson W.C.,
RA   Vamathevan J.J., Tran B., Upton J., Hansen T., Shetty J., Khouri H.M.,
RA   Utterback T.R., Radune D., Ketchum K.A., Dougherty B.A., Fraser C.M.;
RT   "Role of mobile DNA in the evolution of vancomycin-resistant Enterococcus
RT   faecalis.";
RL   Science 299:2071-2074(2003).
CC   -!- FUNCTION: Catalyzes the attachment of proline to tRNA(Pro) in a two-
CC       step reaction: proline is first activated by ATP to form Pro-AMP and
CC       then transferred to the acceptor end of tRNA(Pro). As ProRS can
CC       inadvertently accommodate and process non-cognate amino acids such as
CC       alanine and cysteine, to avoid such errors it has two additional
CC       distinct editing activities against alanine. One activity is designated
CC       as 'pretransfer' editing and involves the tRNA(Pro)-independent
CC       hydrolysis of activated Ala-AMP. The other activity is designated
CC       'posttransfer' editing and involves deacylation of mischarged Ala-
CC       tRNA(Pro). The misacylated Cys-tRNA(Pro) is not edited by ProRS.
CC       {ECO:0000255|HAMAP-Rule:MF_01569}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-proline + tRNA(Pro) = AMP + diphosphate + L-prolyl-
CC         tRNA(Pro); Xref=Rhea:RHEA:14305, Rhea:RHEA-COMP:9700, Rhea:RHEA-
CC         COMP:9702, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019, ChEBI:CHEBI:60039,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78532, ChEBI:CHEBI:456215;
CC         EC=6.1.1.15; Evidence={ECO:0000255|HAMAP-Rule:MF_01569};
CC   -!- SUBUNIT: Homodimer. {ECO:0000255|HAMAP-Rule:MF_01569}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_01569}.
CC   -!- DOMAIN: Consists of three domains: the N-terminal catalytic domain, the
CC       editing domain and the C-terminal anticodon-binding domain.
CC       {ECO:0000255|HAMAP-Rule:MF_01569}.
CC   -!- SIMILARITY: Belongs to the class-II aminoacyl-tRNA synthetase family.
CC       ProS type 1 subfamily. {ECO:0000255|HAMAP-Rule:MF_01569}.
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DR   EMBL; AE016830; AAO82100.1; -; Genomic_DNA.
DR   RefSeq; NP_816030.1; NC_004668.1.
DR   RefSeq; WP_002382780.1; NZ_KE136528.1.
DR   PDB; 2J3L; X-ray; 2.30 A; A/B=1-572.
DR   PDB; 2J3M; X-ray; 2.30 A; A/B=1-572.
DR   PDBsum; 2J3L; -.
DR   PDBsum; 2J3M; -.
DR   AlphaFoldDB; Q831W7; -.
DR   SMR; Q831W7; -.
DR   DIP; DIP-29057N; -.
DR   STRING; 226185.EF_2379; -.
DR   EnsemblBacteria; AAO82100; AAO82100; EF_2379.
DR   KEGG; efa:EF2379; -.
DR   PATRIC; fig|226185.45.peg.1159; -.
DR   eggNOG; COG0442; Bacteria.
DR   HOGENOM; CLU_016739_0_0_9; -.
DR   OMA; NCDYAAN; -.
DR   BRENDA; 6.1.1.15; 2095.
DR   EvolutionaryTrace; Q831W7; -.
DR   Proteomes; UP000001415; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0002161; F:aminoacyl-tRNA editing activity; IEA:InterPro.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0004827; F:proline-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0006433; P:prolyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   CDD; cd00861; ProRS_anticodon_short; 1.
DR   CDD; cd00779; ProRS_core_prok; 1.
DR   Gene3D; 3.30.930.10; -; 2.
DR   Gene3D; 3.40.50.800; -; 1.
DR   Gene3D; 3.90.960.10; -; 1.
DR   HAMAP; MF_01569; Pro_tRNA_synth_type1; 1.
DR   InterPro; IPR002314; aa-tRNA-synt_IIb.
DR   InterPro; IPR006195; aa-tRNA-synth_II.
DR   InterPro; IPR045864; aa-tRNA-synth_II/BPL/LPL.
DR   InterPro; IPR004154; Anticodon-bd.
DR   InterPro; IPR036621; Anticodon-bd_dom_sf.
DR   InterPro; IPR002316; Pro-tRNA-ligase_IIa.
DR   InterPro; IPR004500; Pro-tRNA-synth_IIa_bac-type.
DR   InterPro; IPR023717; Pro-tRNA-Synthase_IIa_type1.
DR   InterPro; IPR044140; ProRS_anticodon_short.
DR   InterPro; IPR033730; ProRS_core_prok.
DR   InterPro; IPR036754; YbaK/aa-tRNA-synt-asso_dom_sf.
DR   InterPro; IPR007214; YbaK/aa-tRNA-synth-assoc-dom.
DR   Pfam; PF03129; HGTP_anticodon; 1.
DR   Pfam; PF00587; tRNA-synt_2b; 1.
DR   Pfam; PF04073; tRNA_edit; 1.
DR   PRINTS; PR01046; TRNASYNTHPRO.
DR   SUPFAM; SSF55681; SSF55681; 1.
DR   SUPFAM; SSF55826; SSF55826; 1.
DR   TIGRFAMs; TIGR00409; proS_fam_II; 1.
DR   PROSITE; PS50862; AA_TRNA_LIGASE_II; 1.
PE   1: Evidence at protein level;
KW   3D-structure; Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..572
FT                   /note="Proline--tRNA ligase"
FT                   /id="PRO_0000248688"
FT   HELIX           3..5
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   HELIX           21..28
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   STRAND          31..36
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   STRAND          39..42
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   HELIX           44..62
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   TURN            63..65
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   STRAND          73..76
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   HELIX           78..83
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   HELIX           85..88
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   STRAND          95..97
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   STRAND          103..106
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   HELIX           111..121
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   HELIX           125..127
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   STRAND          130..139
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   HELIX           148..150
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   STRAND          153..166
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   HELIX           167..187
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   STRAND          192..196
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   HELIX           199..202
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   STRAND          205..213
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   STRAND          218..228
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   STRAND          230..232
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   TURN            233..235
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   STRAND          253..256
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   HELIX           263..270
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   HELIX           274..276
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   STRAND          277..285
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   STRAND          288..295
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   HELIX           302..309
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   STRAND          314..316
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   HELIX           319..326
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   HELIX           330..332
FT                   /evidence="ECO:0007829|PDB:2J3M"
FT   STRAND          335..337
FT                   /evidence="ECO:0007829|PDB:2J3M"
FT   STRAND          343..347
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   TURN            348..352
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   STRAND          355..359
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   STRAND          365..370
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   TURN            372..374
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   STRAND          379..382
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   STRAND          396..415
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   HELIX           417..422
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   STRAND          425..427
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   STRAND          433..435
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   STRAND          437..444
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   HELIX           445..456
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   TURN            466..468
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   STRAND          472..478
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   HELIX           483..498
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   STRAND          503..506
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   HELIX           512..522
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   STRAND          525..530
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   HELIX           532..536
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   STRAND          538..543
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   TURN            544..546
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   STRAND          549..553
FT                   /evidence="ECO:0007829|PDB:2J3L"
FT   HELIX           554..564
FT                   /evidence="ECO:0007829|PDB:2J3L"
SQ   SEQUENCE   572 AA;  64267 MW;  D2267BE6E4A85998 CRC64;
     MKQSKMLIPT LREVPNDAEV LSHQILLRAG YIRQVAAGIY SYLPLANRVL EKLKTIMREE
     FEKIDAVEML MPALLPAELW KESGRYETYG PNLYRLKDRN DRDYILGPTH EETFTELIRD
     EINSYKRLPL NLYQIQTKYR DEKRSRSGLL RGREFIMKDG YSFHADEASL DQSYRDYEKA
     YSRIFERCGL EFRAIIGDGG AMGGKDSKEF MAISEIGEDT ICYSTESDYA ANLEMATSLY
     TPKKSHETQL DLEKIATPEV GTIAEVANFF EVEPQRIIKS VLFIADEEPV MVLVRGDHDV
     NDVKLKNFLG ADFLDEATEE DARRVLGAGF GSIGPVNVSE DVKIYADLAV QDLANAIVGA
     NEDGYHLTNV NPDRDFQPIS YEDLRFVQEG DPSPDGNGVL AFTKGIEIGH IFKLGTRYSD
     AMGATVLDEN GREKSVIMGC YGIGVSRLLS AIVEQNADER GINWPTGIAP FDLHVVQMNV
     KDEYQTKLSQ EVEAMMTEAG YEVLVDDRNE RAGVKFADAD LIGCPIRITV GKKAVDGVVE
     VKIKRTGEML EVRKEELEST LSILMNTTSE VE
 
 
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