BMP8B_MOUSE
ID BMP8B_MOUSE Reviewed; 399 AA.
AC P55105; Q8BNM2;
DT 01-OCT-1996, integrated into UniProtKB/Swiss-Prot.
DT 27-JUL-2011, sequence version 2.
DT 03-AUG-2022, entry version 151.
DE RecName: Full=Bone morphogenetic protein 8B;
DE Short=BMP-8B;
DE Flags: Precursor;
GN Name=Bmp8b;
OS Mus musculus (Mouse).
OC Eukaryota; Metazoa; Chordata; Craniata; Vertebrata; Euteleostomi; Mammalia;
OC Eutheria; Euarchontoglires; Glires; Rodentia; Myomorpha; Muroidea; Muridae;
OC Murinae; Mus; Mus.
OX NCBI_TaxID=10090;
RN [1]
RP NUCLEOTIDE SEQUENCE [MRNA].
RC STRAIN=ICR; TISSUE=Placenta;
RX PubMed=8843393; DOI=10.1016/0925-4773(96)00543-6;
RA Zhao G.Q., Hogan B.L.;
RT "Evidence that mouse Bmp8a (Op2) and Bmp8b are duplicated genes that play a
RT role in spermatogenesis and placental development.";
RL Mech. Dev. 57:159-168(1996).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=C57BL/6J;
RX PubMed=16141072; DOI=10.1126/science.1112014;
RA Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N.,
RA Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K.,
RA Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.,
RA Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R.,
RA Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T.,
RA Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A.,
RA Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B.,
RA Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M.,
RA Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S.,
RA Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E.,
RA Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D.,
RA Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M.,
RA Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H.,
RA Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V.,
RA Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S.,
RA Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H.,
RA Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N.,
RA Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F.,
RA Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G.,
RA Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z.,
RA Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C.,
RA Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y.,
RA Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S.,
RA Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K.,
RA Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R.,
RA van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H.,
RA Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M.,
RA Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C.,
RA Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S.,
RA Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K.,
RA Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M.,
RA Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C.,
RA Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A.,
RA Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.;
RT "The transcriptional landscape of the mammalian genome.";
RL Science 309:1559-1563(2005).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RA Mural R.J., Adams M.D., Myers E.W., Smith H.O., Venter J.C.;
RL Submitted (SEP-2005) to the EMBL/GenBank/DDBJ databases.
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC TISSUE=Brain;
RX PubMed=15489334; DOI=10.1101/gr.2596504;
RG The MGC Project Team;
RT "The status, quality, and expansion of the NIH full-length cDNA project:
RT the Mammalian Gene Collection (MGC).";
RL Genome Res. 14:2121-2127(2004).
RN [5]
RP FUNCTION.
RX PubMed=8682296; DOI=10.1101/gad.10.13.1657;
RA Zhao G.Q., Deng K., Labosky P.A., Liaw L., Hogan B.L.;
RT "The gene encoding bone morphogenetic protein 8B is required for the
RT initiation and maintenance of spermatogenesis in the mouse.";
RL Genes Dev. 10:1657-1669(1996).
RN [6]
RP FUNCTION, TISSUE SPECIFICITY, AND DISRUPTION PHENOTYPE.
RX PubMed=10894154; DOI=10.1210/mend.14.7.0479;
RA Ying Y., Liu X.M., Marble A., Lawson K.A., Zhao G.Q.;
RT "Requirement of Bmp8b for the generation of primordial germ cells in the
RT mouse.";
RL Mol. Endocrinol. 14:1053-1063(2000).
RN [7]
RP FUNCTION, AND SUBUNIT.
RX PubMed=11427739; DOI=10.1073/pnas.151242798;
RA Ying Y., Qi X., Zhao G.Q.;
RT "Induction of primordial germ cells from murine epiblasts by synergistic
RT action of BMP4 and BMP8B signaling pathways.";
RL Proc. Natl. Acad. Sci. U.S.A. 98:7858-7862(2001).
RN [8]
RP FUNCTION, INDUCTION, AND TISSUE SPECIFICITY.
RX PubMed=22579288; DOI=10.1016/j.cell.2012.02.066;
RA Whittle A.J., Carobbio S., Martins L., Slawik M., Hondares E.,
RA Vazquez M.J., Morgan D., Csikasz R.I., Gallego R., Rodriguez-Cuenca S.,
RA Dale M., Virtue S., Villarroya F., Cannon B., Rahmouni K., Lopez M.,
RA Vidal-Puig A.;
RT "BMP8B increases brown adipose tissue thermogenesis through both central
RT and peripheral actions.";
RL Cell 149:871-885(2012).
CC -!- FUNCTION: Induces cartilage and bone formation. May be the
CC osteoinductive factor responsible for the phenomenon of epithelial
CC osteogenesis. Plays a role in calcium regulation and bone homeostasis
CC (By similarity). Involved in the generation of primordial germ cells;
CC this function involves Bmp4 in a synergistic manner though separate
CC receptor complexes seem to be involved. Required for the initiation and
CC maintenance of spermatogenesis. Signaling protein involved in
CC regulation of thermogenesis and energy balance. Proposed to increase
CC the peripheral response of brown adipose tissue (BAT) to adrenergic
CC stimulation while acting centrally in the hypothalamus to increase
CC sympathetic output to BAT. {ECO:0000250, ECO:0000269|PubMed:10894154,
CC ECO:0000269|PubMed:11427739, ECO:0000269|PubMed:22579288,
CC ECO:0000269|PubMed:8682296}.
CC -!- SUBUNIT: Homodimer; disulfide-linked. {ECO:0000305|PubMed:11427739}.
CC -!- SUBCELLULAR LOCATION: Secreted {ECO:0000250}.
CC -!- TISSUE SPECIFICITY: Expressed in testis. Expressed in decidual cells of
CC the uterus and in trophoblast cells of the labyrinthine region of the
CC placenta and in the inner root sheath of hair follicles of early
CC postnatal skin. Expressed in the extraembryonic ectoderm in pregastrula
CC and gastrula stage mouse embryos. Expressed in brown adipose tissue and
CC brain. {ECO:0000269|PubMed:10894154, ECO:0000269|PubMed:22579288}.
CC -!- DEVELOPMENTAL STAGE: Expressed during specific stages of
CC spermatogenesis, with highest levels in stage 6-8 round spermatids
CC after 3 weeks of age.
CC -!- INDUCTION: By feeding with high-fat diet and cold exposure. By beta-3-
CC adrenergic receptor activation and thyroid hormone treatment.
CC {ECO:0000269|PubMed:22579288}.
CC -!- DISRUPTION PHENOTYPE: Absence of primordial germ cells, short or
CC missing allantois. {ECO:0000269|PubMed:10894154}.
CC -!- SIMILARITY: Belongs to the TGF-beta family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; U39545; AAB17573.1; -; mRNA.
DR EMBL; AK082895; BAC38674.1; -; mRNA.
DR EMBL; CH466552; EDL30390.1; -; Genomic_DNA.
DR EMBL; BC137890; AAI37891.1; -; mRNA.
DR CCDS; CCDS18608.1; -.
DR RefSeq; NP_031585.2; NM_007559.5.
DR AlphaFoldDB; P55105; -.
DR SMR; P55105; -.
DR STRING; 10090.ENSMUSP00000002457; -.
DR GlyGen; P55105; 2 sites.
DR PhosphoSitePlus; P55105; -.
DR MaxQB; P55105; -.
DR PaxDb; P55105; -.
DR PeptideAtlas; P55105; -.
DR PRIDE; P55105; -.
DR ProteomicsDB; 273788; -.
DR DNASU; 12164; -.
DR Ensembl; ENSMUST00000002457; ENSMUSP00000002457; ENSMUSG00000002384.
DR GeneID; 12164; -.
DR KEGG; mmu:12164; -.
DR UCSC; uc008uot.1; mouse.
DR CTD; 656; -.
DR MGI; MGI:107335; Bmp8b.
DR VEuPathDB; HostDB:ENSMUSG00000002384; -.
DR eggNOG; KOG3900; Eukaryota.
DR GeneTree; ENSGT00940000164770; -.
DR HOGENOM; CLU_020515_4_1_1; -.
DR InParanoid; P55105; -.
DR OMA; KVPNDNY; -.
DR OrthoDB; 1063560at2759; -.
DR PhylomeDB; P55105; -.
DR TreeFam; TF316134; -.
DR BioGRID-ORCS; 12164; 3 hits in 74 CRISPR screens.
DR PRO; PR:P55105; -.
DR Proteomes; UP000000589; Chromosome 4.
DR RNAct; P55105; protein.
DR Bgee; ENSMUSG00000002384; Expressed in ectoplacental cone and 40 other tissues.
DR Genevisible; P55105; MM.
DR GO; GO:0005615; C:extracellular space; IBA:GO_Central.
DR GO; GO:0070700; F:BMP receptor binding; IBA:GO_Central.
DR GO; GO:0005125; F:cytokine activity; IBA:GO_Central.
DR GO; GO:0008083; F:growth factor activity; IEA:UniProtKB-KW.
DR GO; GO:0030509; P:BMP signaling pathway; IBA:GO_Central.
DR GO; GO:0051216; P:cartilage development; IEA:UniProtKB-KW.
DR GO; GO:0002024; P:diet induced thermogenesis; IMP:UniProtKB.
DR GO; GO:0048598; P:embryonic morphogenesis; IMP:UniProtKB.
DR GO; GO:0097009; P:energy homeostasis; IMP:UniProtKB.
DR GO; GO:0007281; P:germ cell development; IMP:UniProtKB.
DR GO; GO:0001503; P:ossification; IEA:UniProtKB-KW.
DR GO; GO:0010862; P:positive regulation of pathway-restricted SMAD protein phosphorylation; IMP:UniProtKB.
DR GO; GO:0043408; P:regulation of MAPK cascade; IMP:UniProtKB.
DR GO; GO:0060395; P:SMAD protein signal transduction; IBA:GO_Central.
DR GO; GO:0007283; P:spermatogenesis; IMP:MGI.
DR GO; GO:0007179; P:transforming growth factor beta receptor signaling pathway; TAS:MGI.
DR Gene3D; 2.10.90.10; -; 1.
DR InterPro; IPR029034; Cystine-knot_cytokine.
DR InterPro; IPR001839; TGF-b_C.
DR InterPro; IPR001111; TGF-b_propeptide.
DR InterPro; IPR015615; TGF-beta-rel.
DR InterPro; IPR017948; TGFb_CS.
DR PANTHER; PTHR11848; PTHR11848; 1.
DR Pfam; PF00019; TGF_beta; 1.
DR Pfam; PF00688; TGFb_propeptide; 1.
DR SMART; SM00204; TGFB; 1.
DR SUPFAM; SSF57501; SSF57501; 1.
DR PROSITE; PS00250; TGF_BETA_1; 1.
DR PROSITE; PS51362; TGF_BETA_2; 1.
PE 1: Evidence at protein level;
KW Chondrogenesis; Cytokine; Developmental protein; Differentiation;
KW Disulfide bond; Glycoprotein; Growth factor; Osteogenesis;
KW Reference proteome; Secreted; Signal; Spermatogenesis.
FT SIGNAL 1..19
FT /evidence="ECO:0000255"
FT PROPEP 20..260
FT /evidence="ECO:0000255"
FT /id="PRO_0000033886"
FT CHAIN 261..399
FT /note="Bone morphogenetic protein 8B"
FT /id="PRO_0000033887"
FT CARBOHYD 155
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT CARBOHYD 340
FT /note="N-linked (GlcNAc...) asparagine"
FT /evidence="ECO:0000255"
FT DISULFID 298..364
FT /evidence="ECO:0000250"
FT DISULFID 327..396
FT /evidence="ECO:0000250"
FT DISULFID 331..398
FT /evidence="ECO:0000250"
FT DISULFID 363
FT /note="Interchain"
FT /evidence="ECO:0000250"
FT CONFLICT 158
FT /note="L -> F (in Ref. 1; AAB17573)"
FT /evidence="ECO:0000305"
FT CONFLICT 177
FT /note="F -> S (in Ref. 1; AAB17573)"
FT /evidence="ECO:0000305"
FT CONFLICT 224
FT /note="S -> G (in Ref. 1; AAB17573)"
FT /evidence="ECO:0000305"
FT CONFLICT 300..301
FT /note="RH -> TG (in Ref. 1; AAB17573)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 399 AA; 44943 MW; 204925A3C68DF6B1 CRC64;
MAARPGLLWL LGLALCVLGG GHLSHPPHVF PQRRLGVREP RDMQREIREV LGLPGRPRSR
APVGAAQQPA SAPLFMLDLY RAMTDDSGGG TPQPHLDRAD LIMSFVNIVE RDRTLGYQEP
HWKEFHFDLT QIPAGEAVTA AEFRIYKEPS THPLNTTLHI SMFEVVQEHS NRESDLFFLD
LQTLRSGDEG WLVLDITAAS DRWLLNHHKD LGLRLYVETE DGHSIDPGLA GLLGRQAPRS
RQPFMVGFFR ANQSPVRAPR TARPLKKKQL NQINQLPHSN KHLGILDDGH GSHGREVCRR
HELYVSFRDL GWLDSVIAPQ GYSAYYCAGE CIYPLNSCMN STNHATMQAL VHLMKPDIIP
KVCCVPTELS AISLLYYDRN NNVILRRERN MVVQACGCH
