BMR1_BACSU
ID BMR1_BACSU Reviewed; 389 AA.
AC P33449;
DT 01-FEB-1994, integrated into UniProtKB/Swiss-Prot.
DT 07-JUL-2009, sequence version 2.
DT 03-AUG-2022, entry version 135.
DE RecName: Full=Multidrug resistance protein 1;
DE AltName: Full=Multidrug-efflux transporter 1;
GN Name=bmr; Synonyms=bmr1; OrderedLocusNames=BSU24010;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / BD170;
RX PubMed=1675788; DOI=10.1073/pnas.88.11.4781;
RA Neyfakh A.A., Bidnenko V.E., Chen L.B.;
RT "Efflux-mediated multidrug resistance in Bacillus subtilis: similarities
RT and dissimilarities with the mammalian system.";
RL Proc. Natl. Acad. Sci. U.S.A. 88:4781-4785(1991).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / Marburg / ATCC 6051 / DSM 10 / JCM 1465 / NBRC 13719 / NCIMB
RC 3610 / NRRL NRS-744 / VKM B-501;
RX PubMed=7961792; DOI=10.1016/s0021-9258(18)46956-6;
RA Ahmed M., Borsch C.M., Taylor S.S., Vazquez-Laslop N., Neyfakh A.A.;
RT "A protein that activates expression of a multidrug efflux transporter upon
RT binding the transporter substrates.";
RL J. Biol. Chem. 269:28506-28513(1994).
RN [3]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=168 / JH642;
RX PubMed=8969508; DOI=10.1099/13500872-142-11-3103;
RA Mizuno M., Masuda S., Takemaru K., Hosono S., Sato T., Takeuchi M.,
RA Kobayashi Y.;
RT "Systematic sequencing of the 283 kb 210 degrees-232 degrees region of the
RT Bacillus subtilis genome containing the skin element and many sporulation
RT genes.";
RL Microbiology 142:3103-3111(1996).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [5]
RP SEQUENCE REVISION TO 109.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
CC -!- FUNCTION: Energy-dependent efflux pump responsible for decreased drug
CC accumulation in multi-drug-resistant cells. Probably uses a
CC transmembrane proton gradient as the energy source. Causes the efflux
CC of a variety of toxic substances, including such structurally diverse
CC compounds as ethidium bromide, rhodamine and acridine dyes,
CC tetraphenylphosphonium, puromycin, chloramphenicol, doxorubicin, and
CC fluoroquinolone antibiotics.
CC -!- SUBCELLULAR LOCATION: Cell membrane; Multi-pass membrane protein.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. TCR/Tet
CC family. {ECO:0000305}.
CC ---------------------------------------------------------------------------
CC Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC ---------------------------------------------------------------------------
DR EMBL; M33768; AAA22277.1; -; Genomic_DNA.
DR EMBL; L25604; AAB81539.1; -; Genomic_DNA.
DR EMBL; D84432; BAA12601.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB14332.2; -; Genomic_DNA.
DR PIR; A39705; A39705.
DR RefSeq; NP_390281.2; NC_000964.3.
DR RefSeq; WP_003230328.1; NZ_JNCM01000036.1.
DR AlphaFoldDB; P33449; -.
DR SMR; P33449; -.
DR STRING; 224308.BSU24010; -.
DR TCDB; 2.A.1.2.70; the major facilitator superfamily (mfs).
DR PaxDb; P33449; -.
DR EnsemblBacteria; CAB14332; CAB14332; BSU_24010.
DR GeneID; 938680; -.
DR KEGG; bsu:BSU24010; -.
DR PATRIC; fig|224308.179.peg.2615; -.
DR eggNOG; COG2814; Bacteria.
DR InParanoid; P33449; -.
DR OMA; STFVGCW; -.
DR PhylomeDB; P33449; -.
DR BioCyc; BSUB:BSU24010-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR GO; GO:0005886; C:plasma membrane; IEA:UniProtKB-SubCell.
DR GO; GO:0022857; F:transmembrane transporter activity; IEA:InterPro.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR InterPro; IPR005829; Sugar_transporter_CS.
DR InterPro; IPR001958; Tet-R_TetA/multi-R_MdtG.
DR Pfam; PF07690; MFS_1; 1.
DR PRINTS; PR01035; TCRTETA.
DR SUPFAM; SSF103473; SSF103473; 1.
DR PROSITE; PS50850; MFS; 1.
DR PROSITE; PS00216; SUGAR_TRANSPORT_1; 1.
PE 3: Inferred from homology;
KW Antibiotic resistance; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..389
FT /note="Multidrug resistance protein 1"
FT /id="PRO_0000173317"
FT TRANSMEM 6..26
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 42..62
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 71..91
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 102..122
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 134..154
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 160..180
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 202..222
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 243..263
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 286..306
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 336..356
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 358..378
FT /note="Helical"
FT /evidence="ECO:0000255"
FT CONFLICT 109
FT /note="A -> P (in Ref. 1; AAA22277, 2; AAB81539 and 3;
FT BAA12601)"
FT /evidence="ECO:0000305"
SQ SEQUENCE 389 AA; 42232 MW; 3E0318AB533D1197 CRC64;
MEKKNITLTI LLTNLFIAFL GIGLVIPVTP TIMNELHLSG TAVGYMVACF AITQLIVSPI
AGRWVDRFGR KIMIVIGLLF FSVSEFLFGI GKTVEMLFIS RMLGGISAAF IMPGVTAFIA
DITTIKTRPK ALGYMSAAIS TGFIIGPGIG GFLAEVHSRL PFFFAAAFAL LAAILSILTL
REPERNPENQ EIKGQKTGFK RIFAPMYFIA FLIILISSFG LASFESLFAL FVDHKFGFTA
SDIAIMITGG AIVGAITQVV LFDRFTRWFG EIHLIRYSLI LSTSLVFLLT TVHSYVAILL
VTVTVFVGFD LMRPAVTTYL SKIAGNEQGF AGGMNSMFTS IGNVFGPIIG GMLFDIDVNY
PFYFATVTLA IGIALTIAWK APAHLKAST
