BMR3_BACSU
ID BMR3_BACSU Reviewed; 512 AA.
AC P96712; O31471;
DT 26-MAY-2009, integrated into UniProtKB/Swiss-Prot.
DT 01-MAY-1997, sequence version 1.
DT 03-AUG-2022, entry version 119.
DE RecName: Full=Multidrug resistance protein 3;
DE AltName: Full=Multidrug-efflux transporter 3;
GN Name=bmr3; Synonyms=mdr; OrderedLocusNames=BSU03070;
OS Bacillus subtilis (strain 168).
OC Bacteria; Firmicutes; Bacilli; Bacillales; Bacillaceae; Bacillus.
OX NCBI_TaxID=224308;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], FUNCTION AS A TRANSPORTER, AND
RP INDUCTION.
RC STRAIN=168;
RX PubMed=9023234; DOI=10.1128/jb.179.4.1423-1427.1997;
RA Ohki R., Murata M.;
RT "bmr3, a third multidrug transporter gene of Bacillus subtilis.";
RL J. Bacteriol. 179:1423-1427(1997).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=168;
RX PubMed=9384377; DOI=10.1038/36786;
RA Kunst F., Ogasawara N., Moszer I., Albertini A.M., Alloni G., Azevedo V.,
RA Bertero M.G., Bessieres P., Bolotin A., Borchert S., Borriss R.,
RA Boursier L., Brans A., Braun M., Brignell S.C., Bron S., Brouillet S.,
RA Bruschi C.V., Caldwell B., Capuano V., Carter N.M., Choi S.-K.,
RA Codani J.-J., Connerton I.F., Cummings N.J., Daniel R.A., Denizot F.,
RA Devine K.M., Duesterhoeft A., Ehrlich S.D., Emmerson P.T., Entian K.-D.,
RA Errington J., Fabret C., Ferrari E., Foulger D., Fritz C., Fujita M.,
RA Fujita Y., Fuma S., Galizzi A., Galleron N., Ghim S.-Y., Glaser P.,
RA Goffeau A., Golightly E.J., Grandi G., Guiseppi G., Guy B.J., Haga K.,
RA Haiech J., Harwood C.R., Henaut A., Hilbert H., Holsappel S., Hosono S.,
RA Hullo M.-F., Itaya M., Jones L.-M., Joris B., Karamata D., Kasahara Y.,
RA Klaerr-Blanchard M., Klein C., Kobayashi Y., Koetter P., Koningstein G.,
RA Krogh S., Kumano M., Kurita K., Lapidus A., Lardinois S., Lauber J.,
RA Lazarevic V., Lee S.-M., Levine A., Liu H., Masuda S., Mauel C.,
RA Medigue C., Medina N., Mellado R.P., Mizuno M., Moestl D., Nakai S.,
RA Noback M., Noone D., O'Reilly M., Ogawa K., Ogiwara A., Oudega B.,
RA Park S.-H., Parro V., Pohl T.M., Portetelle D., Porwollik S.,
RA Prescott A.M., Presecan E., Pujic P., Purnelle B., Rapoport G., Rey M.,
RA Reynolds S., Rieger M., Rivolta C., Rocha E., Roche B., Rose M., Sadaie Y.,
RA Sato T., Scanlan E., Schleich S., Schroeter R., Scoffone F., Sekiguchi J.,
RA Sekowska A., Seror S.J., Serror P., Shin B.-S., Soldo B., Sorokin A.,
RA Tacconi E., Takagi T., Takahashi H., Takemaru K., Takeuchi M.,
RA Tamakoshi A., Tanaka T., Terpstra P., Tognoni A., Tosato V., Uchiyama S.,
RA Vandenbol M., Vannier F., Vassarotti A., Viari A., Wambutt R., Wedler E.,
RA Wedler H., Weitzenegger T., Winters P., Wipat A., Yamamoto H., Yamane K.,
RA Yasumoto K., Yata K., Yoshida K., Yoshikawa H.-F., Zumstein E.,
RA Yoshikawa H., Danchin A.;
RT "The complete genome sequence of the Gram-positive bacterium Bacillus
RT subtilis.";
RL Nature 390:249-256(1997).
RN [3]
RP SEQUENCE REVISION.
RX PubMed=19383706; DOI=10.1099/mic.0.027839-0;
RA Barbe V., Cruveiller S., Kunst F., Lenoble P., Meurice G., Sekowska A.,
RA Vallenet D., Wang T., Moszer I., Medigue C., Danchin A.;
RT "From a consortium sequence to a unified sequence: the Bacillus subtilis
RT 168 reference genome a decade later.";
RL Microbiology 155:1758-1775(2009).
CC -!- FUNCTION: Confers resistance to puromycin, tosufloxacin and
CC norfloxacin. {ECO:0000269|PubMed:9023234}.
CC -!- SUBCELLULAR LOCATION: Cell membrane {ECO:0000305}; Multi-pass membrane
CC protein {ECO:0000305}.
CC -!- INDUCTION: Expression is dependent on the growth phase and decreases in
CC the late log phase. {ECO:0000269|PubMed:9023234}.
CC -!- SIMILARITY: Belongs to the major facilitator superfamily. EmrB family.
CC {ECO:0000305}.
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DR EMBL; D50098; BAA08793.1; -; Genomic_DNA.
DR EMBL; AL009126; CAB12101.2; -; Genomic_DNA.
DR RefSeq; NP_388189.2; NC_000964.3.
DR RefSeq; WP_003246377.1; NZ_JNCM01000030.1.
DR AlphaFoldDB; P96712; -.
DR SMR; P96712; -.
DR STRING; 224308.BSU03070; -.
DR TCDB; 2.A.1.3.50; the major facilitator superfamily (mfs).
DR PRIDE; P96712; -.
DR EnsemblBacteria; CAB12101; CAB12101; BSU_03070.
DR GeneID; 938345; -.
DR KEGG; bsu:BSU03070; -.
DR PATRIC; fig|224308.179.peg.321; -.
DR eggNOG; COG2814; Bacteria.
DR InParanoid; P96712; -.
DR OMA; IPGWVWR; -.
DR PhylomeDB; P96712; -.
DR BioCyc; BSUB:BSU03070-MON; -.
DR Proteomes; UP000001570; Chromosome.
DR GO; GO:0005887; C:integral component of plasma membrane; IBA:GO_Central.
DR GO; GO:0022857; F:transmembrane transporter activity; IBA:GO_Central.
DR GO; GO:0046677; P:response to antibiotic; IEA:UniProtKB-KW.
DR GO; GO:0055085; P:transmembrane transport; IBA:GO_Central.
DR Gene3D; 1.20.1250.20; -; 1.
DR InterPro; IPR004638; EmrB-like.
DR InterPro; IPR011701; MFS.
DR InterPro; IPR020846; MFS_dom.
DR InterPro; IPR036259; MFS_trans_sf.
DR Pfam; PF07690; MFS_1; 1.
DR SUPFAM; SSF103473; SSF103473; 1.
DR TIGRFAMs; TIGR00711; efflux_EmrB; 1.
DR PROSITE; PS50850; MFS; 1.
PE 1: Evidence at protein level;
KW Antibiotic resistance; Cell membrane; Membrane; Reference proteome;
KW Transmembrane; Transmembrane helix; Transport.
FT CHAIN 1..512
FT /note="Multidrug resistance protein 3"
FT /id="PRO_0000375916"
FT TRANSMEM 13..33
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 48..68
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 79..99
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 109..129
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 139..159
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 163..183
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 200..220
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 228..248
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 272..292
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 304..324
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 333..353
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 358..378
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 399..421
FT /note="Helical"
FT /evidence="ECO:0000255"
FT TRANSMEM 475..495
FT /note="Helical"
FT /evidence="ECO:0000255"
SQ SEQUENCE 512 AA; 55689 MW; D789A593F2ADB262 CRC64;
MDTTTAKQAS TKFVVLGLLL GILMSAMDNT IVATAMGNIV ADLGSFDKFA WVTASYMVAV
MAGMPIYGKL SDMYGRKRFF LFGLIFFLIG SALCGIAQTM NQLIIFRAIQ GIGGGALLPI
AFTIIFDLFP PEKRGKMSGM FGAVFGLSSV LGPLLGAIIT DSISWHWVFY INVPIGALSL
FFIIRYYKES LEHRKQKIDW GGAITLVVSI VCLMFALELG GKTYDWNSIQ IIGLFIVFAV
FFIAFFIVER KAEEPIISFW MFKNRLFATA QILAFLYGGT FIILAVFIPI FVQAVYGSSA
TSAGFILTPM MIGSVIGSMI GGIFQTKASF RNLMLISVIA FFIGMLLLSN MTPDTARVWL
TVFMMISGFG VGFNFSLLPA ASMNDLEPRF RGTANSTNSF LRSFGMTLGV TIFGTVQTNV
FTNKLNDAFS GMKGSAGSGA AQNIGDPQEI FQAGTRSQIP DAILNRIIDA MSSSITYVFL
LALIPIVLAA VTILFMGKAR VKTTAEMTKK AN
