ID   BMT1_KOMPC              Reviewed;         652 AA.
AC   F2QZ65;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   16-APR-2014, sequence version 2.
DT   25-MAY-2022, entry version 42.
DE   RecName: Full=Beta-mannosyltransferase 1;
DE            EC=2.4.1.-;
GN   Name=BMT1; OrderedLocusNames=PP7435_Chr4-0529;
OS   Komagataella phaffii (strain ATCC 76273 / CBS 7435 / CECT 11047 / NRRL
OS   Y-11430 / Wegner 21-1) (Yeast) (Pichia pastoris).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Phaffomycetaceae; Komagataella.
OX   NCBI_TaxID=981350;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 76273 / CBS 7435 / CECT 11047 / NRRL Y-11430 / Wegner 21-1;
RX   PubMed=21575661; DOI=10.1016/j.jbiotec.2011.04.014;
RA   Kueberl A., Schneider J., Thallinger G.G., Anderl I., Wibberg D., Hajek T.,
RA   Jaenicke S., Brinkrolf K., Goesmann A., Szczepanowski R., Puehler A.,
RA   Schwab H., Glieder A., Pichler H.;
RT   "High-quality genome sequence of Pichia pastoris CBS7435.";
RL   J. Biotechnol. 154:312-320(2011).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 76273 / CBS 7435 / CECT 11047 / NRRL Y-11430 / Wegner 21-1;
RX   PubMed=27388471; DOI=10.1093/femsyr/fow051;
RA   Valli M., Tatto N.E., Peymann A., Gruber C., Landes N., Ekker H.,
RA   Thallinger G.G., Mattanovich D., Gasser B., Graf A.B.;
RT   "Curation of the genome annotation of Pichia pastoris (Komagataella
RT   phaffii) CBS7435 from gene level to protein function.";
RL   FEMS Yeast Res. 16:0-0(2016).
CC   -!- FUNCTION: Beta-mannosyltransferase involved in cell wall biosynthesis.
CC       Involved in the beta-mannosylation of outer chains of N-glycans (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type II
CC       membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the BMT family. {ECO:0000305}.
CC   -!- SEQUENCE CAUTION:
CC       Sequence=CCA40693.1; Type=Erroneous initiation; Note=Truncated N-terminus.; Evidence={ECO:0000305};
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DR   EMBL; FR839631; CCA40693.1; ALT_INIT; Genomic_DNA.
DR   AlphaFoldDB; F2QZ65; -.
DR   SMR; F2QZ65; -.
DR   CAZy; GT91; Glycosyltransferase Family 91.
DR   EnsemblFungi; CCA40693; CCA40693; PP7435_CHR4-0529.
DR   HOGENOM; CLU_013841_3_0_1; -.
DR   Proteomes; UP000006853; Chromosome 4.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0000030; F:mannosyltransferase activity; IEA:InterPro.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   InterPro; IPR021988; BMT1.
DR   PANTHER; PTHR37989; PTHR37989; 2.
DR   Pfam; PF12141; DUF3589; 2.
PE   3: Inferred from homology;
KW   Cell wall biogenesis/degradation; Coiled coil; Glycoprotein;
KW   Glycosyltransferase; Membrane; Signal-anchor; Transferase; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..652
FT                   /note="Beta-mannosyltransferase 1"
FT                   /id="PRO_0000426099"
FT   TOPO_DOM        1..15
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        16..34
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        35..652
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   REGION          536..621
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          535..652
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        57
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   652 AA;  74338 MW;  F03E379001B1058A CRC64;
     MVDLFQWLKF YSMRRLGQVA ITLVLLNLFV FLGYKFTPST VIGSPSWEPA VVPTVFNESY
     LDSLQFTDIN VDSFLSDTNG RISVTCDSLA YKGLVKTSKK KELDCDMAYI RRKIFSSEEY
     GVLADLEAQD ITEEQRIKKH WFTFYGSSVY LPEHEVHYLV RRVLFSKVGR ADTPVISLLV
     AQLYDKDWNE LTPHTLEIVN PATGNVTPQT FPQLIHVPIE WSVDDKWKGT EDPRVFLKPS
     KTGVSEPIVL FNLQSSLCDG KRGMFVTSPF RSDKVNLLDI EDKERPNSEK NWSPFFLDDV
     EVSKYSTGYV HFVYSFNPLK VIKCSLDTGA CRMIYESPEE GRFGSELRGA TPMVKLPVHL
     SLPKGKEVWV AFPRTRLRDC GCSRTTYRPV LTLFVKEGNK FYTELISSSI DFHIDVLSYD
     AKGESCSGSI SVLIPNGIDS WDVSKKQGGK SDILTLTLSE ADRNTVVVHV KGLLDYLLVL
     NGEGPIHDSH SFKNVLSTNH FKSDTTLLNS VKAAECAIFS SRDYCKKYGE TRGEPARYAK
     QMENERKEKE KKEKEAKEKL EAEKAEMEEA VRKAQEAIAQ KEREKEEAEQ EKKAQQEAKE
     KEAEEKAAKE KEAKENEAKK KIIVEKLAKE QEEAEKLEAK KKLYQLQEEE RS