SYQ_ENTBH
ID SYQ_ENTBH Reviewed; 691 AA.
AC A9CSU5;
DT 03-NOV-2009, integrated into UniProtKB/Swiss-Prot.
DT 05-FEB-2008, sequence version 1.
DT 03-AUG-2022, entry version 62.
DE RecName: Full=Probable glutamine--tRNA ligase;
DE EC=6.1.1.18 {ECO:0000250|UniProtKB:P47897};
DE AltName: Full=Glutaminyl-tRNA synthetase;
DE Short=GlnRS;
GN ORFNames=EBI_22570;
OS Enterocytozoon bieneusi (strain H348) (Microsporidian parasite).
OC Eukaryota; Fungi; Fungi incertae sedis; Microsporidia; Enterocytozoonidae;
OC Enterocytozoon.
OX NCBI_TaxID=481877;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=H348;
RX PubMed=18060071; DOI=10.1371/journal.pone.0001277;
RA Corradi N., Akiyoshi D.E., Morrison H.G., Feng X., Weiss L.M., Tzipori S.,
RA Keeling P.J.;
RT "Patterns of genome evolution among the microsporidian parasites
RT Encephalitozoon cuniculi, Antonospora locustae and Enterocytozoon
RT bieneusi.";
RL PLoS ONE 2:E1277-E1277(2007).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=H348;
RX PubMed=19132089; DOI=10.1371/journal.ppat.1000261;
RA Akiyoshi D.E., Morrison H.G., Lei S., Feng X., Zhang Q., Corradi N.,
RA Mayanja H., Tumwine J.K., Keeling P.J., Weiss L.M., Tzipori S.;
RT "Genomic survey of the non-cultivatable opportunistic human pathogen,
RT Enterocytozoon bieneusi.";
RL PLoS Pathog. 5:E1000261-E1000261(2009).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-
CC glutaminyl-tRNA(Gln); Xref=Rhea:RHEA:20121, Rhea:RHEA-COMP:9662,
CC Rhea:RHEA-COMP:9681, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58359, ChEBI:CHEBI:78442, ChEBI:CHEBI:78521,
CC ChEBI:CHEBI:456215; EC=6.1.1.18;
CC Evidence={ECO:0000250|UniProtKB:P47897};
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000305}.
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DR EMBL; ABGB01000003; EDQ31158.1; -; Genomic_DNA.
DR RefSeq; XP_001827993.1; XM_001827941.1.
DR AlphaFoldDB; A9CSU5; -.
DR SMR; A9CSU5; -.
DR STRING; 481877.A9CSU5; -.
DR EnsemblFungi; EDQ31158; EDQ31158; EBI_22570.
DR VEuPathDB; MicrosporidiaDB:EBI_22570; -.
DR HOGENOM; CLU_001882_2_3_1; -.
DR InParanoid; A9CSU5; -.
DR Proteomes; UP000001742; Unassembled WGS sequence.
DR GO; GO:0005737; C:cytoplasm; IEA:InterPro.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-KW.
DR GO; GO:0004819; F:glutamine-tRNA ligase activity; IEA:UniProtKB-EC.
DR GO; GO:0006418; P:tRNA aminoacylation for protein translation; IEA:InterPro.
DR Gene3D; 2.40.240.10; -; 2.
DR Gene3D; 3.40.50.620; -; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR000924; Glu/Gln-tRNA-synth.
DR InterPro; IPR020058; Glu/Gln-tRNA-synth_Ib_cat-dom.
DR InterPro; IPR020059; Glu/Gln-tRNA-synth_Ib_codon-bd.
DR InterPro; IPR020056; Rbsml_L25/Gln-tRNA_synth_N.
DR InterPro; IPR011035; Ribosomal_L25/Gln-tRNA_synth.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR Pfam; PF00749; tRNA-synt_1c; 1.
DR Pfam; PF03950; tRNA-synt_1c_C; 1.
DR PRINTS; PR00987; TRNASYNTHGLU.
DR SUPFAM; SSF50715; SSF50715; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 3: Inferred from homology;
KW Aminoacyl-tRNA synthetase; ATP-binding; Ligase; Nucleotide-binding;
KW Protein biosynthesis; Reference proteome.
FT CHAIN 1..691
FT /note="Probable glutamine--tRNA ligase"
FT /id="PRO_0000388382"
FT MOTIF 196..206
FT /note="'HIGH' region"
FT /evidence="ECO:0000250"
FT MOTIF 436..440
FT /note="'KMSKS' region"
FT /evidence="ECO:0000250"
FT BINDING 197..199
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:P00962"
FT BINDING 203..209
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:P00962"
FT BINDING 229
FT /ligand="L-glutamine"
FT /ligand_id="ChEBI:CHEBI:58359"
FT /evidence="ECO:0000250|UniProtKB:P00962"
FT BINDING 381
FT /ligand="L-glutamine"
FT /ligand_id="ChEBI:CHEBI:58359"
FT /evidence="ECO:0000250|UniProtKB:P00962"
FT BINDING 429..430
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:P00962"
FT BINDING 437..439
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000250|UniProtKB:P00962"
SQ SEQUENCE 691 AA; 80660 MW; 1F11DE3091ADF9CF CRC64;
MNLDDKLRGI SLSDEKINEL KTKPNLIKNM EVIVNTGNNF TKMQYSLACI APKNCNLIEL
SNLIEEGYVT NDSLLKGLVK LGKKSKDEYI DFCKKHTYNE QDIINFIRLL KEKKETKATI
SSKLKEQYLY YDTKIFLKEM ENYNLNVPME KYTKNWLDEG EIKFLHKPGE NPQKTKKIME
DHLKRTGGKV ITRFPPEPNG NLHIGHAKAL NLSFEYAKKF NGITYLRFDD TNPKNESNEL
YNGIIEDVKW LGFEPYAITA SSDHFEAMHE MAKTLIKKNK SYVCFCSLEE IRARRSKYQK
ERDEGNDDPL ILSPYRNTSV EQNLIEFEKM LKGEYKDGEA VLRFKMPLKS KNPLMLDLVG
ARIINMVHDR KQKNYIVYPS YEFALCVCDS LEDITHSFCS REFYTRQEPY HWLLQELDMY
EPVQWEFSRL NISNTVLSKR KLTKIVDEGL NWDDPRFYTI KGMRRRGFPA SAINKFVQSV
GITFSETIID VKILESFVLK ELMQIAKKAT CIINPLKVYI NKCTTGIISN EPVDVNNIIY
IDKDDFDETN NSDFLRLTKI QPVGLINLGV LKYIKDESDG IRCELLTVDE CKPNKFIQWL
PNLDNKVELR MYKPLFKSFD PDEIGYMNDI DLTNSLEIID GYVDNTVLNT HIEDKFQFIR
IGFFCCDFDS IINDNQKKLV FNLTLPLNKN Y