SYQ_PSEAE
ID SYQ_PSEAE Reviewed; 556 AA.
AC Q9I2U8;
DT 02-AUG-2002, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 1.
DT 03-AUG-2022, entry version 125.
DE RecName: Full=Glutamine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00126};
DE EC=6.1.1.18 {ECO:0000255|HAMAP-Rule:MF_00126};
DE AltName: Full=Glutaminyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00126};
DE Short=GlnRS {ECO:0000255|HAMAP-Rule:MF_00126};
GN Name=glnS {ECO:0000255|HAMAP-Rule:MF_00126}; OrderedLocusNames=PA1794;
OS Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM
OS 14847 / LMG 12228 / 1C / PRS 101 / PAO1).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Pseudomonadales;
OC Pseudomonadaceae; Pseudomonas.
OX NCBI_TaxID=208964;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C /
RC PRS 101 / PAO1;
RX PubMed=10984043; DOI=10.1038/35023079;
RA Stover C.K., Pham X.-Q.T., Erwin A.L., Mizoguchi S.D., Warrener P.,
RA Hickey M.J., Brinkman F.S.L., Hufnagle W.O., Kowalik D.J., Lagrou M.,
RA Garber R.L., Goltry L., Tolentino E., Westbrock-Wadman S., Yuan Y.,
RA Brody L.L., Coulter S.N., Folger K.R., Kas A., Larbig K., Lim R.M.,
RA Smith K.A., Spencer D.H., Wong G.K.-S., Wu Z., Paulsen I.T., Reizer J.,
RA Saier M.H. Jr., Hancock R.E.W., Lory S., Olson M.V.;
RT "Complete genome sequence of Pseudomonas aeruginosa PAO1, an opportunistic
RT pathogen.";
RL Nature 406:959-964(2000).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-glutamine + tRNA(Gln) = AMP + diphosphate + L-
CC glutaminyl-tRNA(Gln); Xref=Rhea:RHEA:20121, Rhea:RHEA-COMP:9662,
CC Rhea:RHEA-COMP:9681, ChEBI:CHEBI:30616, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:58359, ChEBI:CHEBI:78442, ChEBI:CHEBI:78521,
CC ChEBI:CHEBI:456215; EC=6.1.1.18; Evidence={ECO:0000255|HAMAP-
CC Rule:MF_00126};
CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00126}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00126}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00126}.
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DR EMBL; AE004091; AAG05183.1; -; Genomic_DNA.
DR PIR; F83421; F83421.
DR RefSeq; NP_250485.1; NC_002516.2.
DR RefSeq; WP_003113586.1; NZ_QZGE01000003.1.
DR PDB; 5BNZ; X-ray; 1.90 A; A/B=1-556.
DR PDBsum; 5BNZ; -.
DR AlphaFoldDB; Q9I2U8; -.
DR SMR; Q9I2U8; -.
DR STRING; 287.DR97_92; -.
DR PaxDb; Q9I2U8; -.
DR PRIDE; Q9I2U8; -.
DR EnsemblBacteria; AAG05183; AAG05183; PA1794.
DR GeneID; 878828; -.
DR KEGG; pae:PA1794; -.
DR PATRIC; fig|208964.12.peg.1861; -.
DR PseudoCAP; PA1794; -.
DR HOGENOM; CLU_001882_2_3_6; -.
DR InParanoid; Q9I2U8; -.
DR OMA; INNFCAQ; -.
DR PhylomeDB; Q9I2U8; -.
DR BioCyc; PAER208964:G1FZ6-1826-MON; -.
DR Proteomes; UP000002438; Chromosome.
DR GO; GO:0005829; C:cytosol; IBA:GO_Central.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0004819; F:glutamine-tRNA ligase activity; IBA:GO_Central.
DR GO; GO:0006425; P:glutaminyl-tRNA aminoacylation; IBA:GO_Central.
DR GO; GO:0006424; P:glutamyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR Gene3D; 1.10.1160.10; -; 1.
DR Gene3D; 2.40.240.10; -; 2.
DR Gene3D; 3.40.50.620; -; 1.
DR HAMAP; MF_00126; Gln_tRNA_synth; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR004514; Gln-tRNA-synth.
DR InterPro; IPR022861; Gln_tRNA_ligase_bac.
DR InterPro; IPR000924; Glu/Gln-tRNA-synth.
DR InterPro; IPR020058; Glu/Gln-tRNA-synth_Ib_cat-dom.
DR InterPro; IPR020059; Glu/Gln-tRNA-synth_Ib_codon-bd.
DR InterPro; IPR020061; Glu_tRNA_lig_a-bdl.
DR InterPro; IPR020056; Rbsml_L25/Gln-tRNA_synth_N.
DR InterPro; IPR011035; Ribosomal_L25/Gln-tRNA_synth.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR Pfam; PF00749; tRNA-synt_1c; 1.
DR Pfam; PF03950; tRNA-synt_1c_C; 1.
DR PRINTS; PR00987; TRNASYNTHGLU.
DR SUPFAM; SSF50715; SSF50715; 1.
DR TIGRFAMs; TIGR00440; glnS; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT CHAIN 1..556
FT /note="Glutamine--tRNA ligase"
FT /id="PRO_0000195843"
FT MOTIF 35..45
FT /note="'HIGH' region"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00126"
FT MOTIF 269..273
FT /note="'KMSKS' region"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00126"
FT BINDING 36..38
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00126"
FT BINDING 42..48
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00126"
FT BINDING 68
FT /ligand="L-glutamine"
FT /ligand_id="ChEBI:CHEBI:58359"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00126"
FT BINDING 213
FT /ligand="L-glutamine"
FT /ligand_id="ChEBI:CHEBI:58359"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00126"
FT BINDING 232
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00126"
FT BINDING 262..263
FT /ligand="ATP"
FT /ligand_id="ChEBI:CHEBI:30616"
FT /evidence="ECO:0000255|HAMAP-Rule:MF_00126"
FT HELIX 12..22
FT /evidence="ECO:0007829|PDB:5BNZ"
FT STRAND 30..33
FT /evidence="ECO:0007829|PDB:5BNZ"
FT STRAND 37..39
FT /evidence="ECO:0007829|PDB:5BNZ"
FT HELIX 43..58
FT /evidence="ECO:0007829|PDB:5BNZ"
FT STRAND 62..67
FT /evidence="ECO:0007829|PDB:5BNZ"
FT HELIX 76..89
FT /evidence="ECO:0007829|PDB:5BNZ"
FT STRAND 95..97
FT /evidence="ECO:0007829|PDB:5BNZ"
FT HELIX 101..104
FT /evidence="ECO:0007829|PDB:5BNZ"
FT HELIX 105..117
FT /evidence="ECO:0007829|PDB:5BNZ"
FT STRAND 121..124
FT /evidence="ECO:0007829|PDB:5BNZ"
FT HELIX 128..135
FT /evidence="ECO:0007829|PDB:5BNZ"
FT STRAND 138..140
FT /evidence="ECO:0007829|PDB:5BNZ"
FT TURN 146..149
FT /evidence="ECO:0007829|PDB:5BNZ"
FT HELIX 152..163
FT /evidence="ECO:0007829|PDB:5BNZ"
FT STRAND 173..176
FT /evidence="ECO:0007829|PDB:5BNZ"
FT HELIX 185..187
FT /evidence="ECO:0007829|PDB:5BNZ"
FT STRAND 191..195
FT /evidence="ECO:0007829|PDB:5BNZ"
FT TURN 201..203
FT /evidence="ECO:0007829|PDB:5BNZ"
FT STRAND 208..211
FT /evidence="ECO:0007829|PDB:5BNZ"
FT HELIX 213..224
FT /evidence="ECO:0007829|PDB:5BNZ"
FT STRAND 227..232
FT /evidence="ECO:0007829|PDB:5BNZ"
FT HELIX 233..238
FT /evidence="ECO:0007829|PDB:5BNZ"
FT HELIX 239..248
FT /evidence="ECO:0007829|PDB:5BNZ"
FT STRAND 256..260
FT /evidence="ECO:0007829|PDB:5BNZ"
FT HELIX 272..280
FT /evidence="ECO:0007829|PDB:5BNZ"
FT STRAND 283..286
FT /evidence="ECO:0007829|PDB:5BNZ"
FT STRAND 292..294
FT /evidence="ECO:0007829|PDB:5BNZ"
FT HELIX 295..301
FT /evidence="ECO:0007829|PDB:5BNZ"
FT HELIX 305..315
FT /evidence="ECO:0007829|PDB:5BNZ"
FT HELIX 327..340
FT /evidence="ECO:0007829|PDB:5BNZ"
FT STRAND 346..355
FT /evidence="ECO:0007829|PDB:5BNZ"
FT STRAND 363..370
FT /evidence="ECO:0007829|PDB:5BNZ"
FT STRAND 372..374
FT /evidence="ECO:0007829|PDB:5BNZ"
FT STRAND 378..390
FT /evidence="ECO:0007829|PDB:5BNZ"
FT HELIX 391..393
FT /evidence="ECO:0007829|PDB:5BNZ"
FT STRAND 410..413
FT /evidence="ECO:0007829|PDB:5BNZ"
FT STRAND 418..426
FT /evidence="ECO:0007829|PDB:5BNZ"
FT STRAND 432..440
FT /evidence="ECO:0007829|PDB:5BNZ"
FT STRAND 460..462
FT /evidence="ECO:0007829|PDB:5BNZ"
FT STRAND 466..473
FT /evidence="ECO:0007829|PDB:5BNZ"
FT STRAND 476..481
FT /evidence="ECO:0007829|PDB:5BNZ"
FT HELIX 491..494
FT /evidence="ECO:0007829|PDB:5BNZ"
FT STRAND 499..508
FT /evidence="ECO:0007829|PDB:5BNZ"
FT HELIX 510..514
FT /evidence="ECO:0007829|PDB:5BNZ"
FT STRAND 520..523
FT /evidence="ECO:0007829|PDB:5BNZ"
FT TURN 524..526
FT /evidence="ECO:0007829|PDB:5BNZ"
FT STRAND 527..531
FT /evidence="ECO:0007829|PDB:5BNZ"
FT TURN 533..535
FT /evidence="ECO:0007829|PDB:5BNZ"
FT STRAND 543..547
FT /evidence="ECO:0007829|PDB:5BNZ"
SQ SEQUENCE 556 AA; 62913 MW; EE75B1BB2C0A77F1 CRC64;
MSKPETTAAP NFLRQIVQAD LDAGKHAKIV TRFPPEPNGY LHIGHAKSIC LNFGLAQEFA
GDCHLRFDDT NPAKEDQEYI DAIEADIKWL GFQWSGEVCY ASNYFDQLHA WAVELIKAGK
AFVCDLGPEE MREYRGTLTE PGRNSPYRDR SVEENLDLFA RMKAGEFPDG ARSLRAKIDM
GSPNMNLRDP ILYRIRHAHH HQTGDKWCIY PSYDFTHGQS DAIEGITHSI CTLEFEDHRP
LYEWFLANLP VPAQPRQYEF SRLNLNYTVT SKRKLKQLVD EGHVSGWDDP RMSTLSGYRR
RGYTPESIRN FCEMIGVNRA SGVVDIGMLE FSIRDHLDAT APRAMCVLKP LKVVITNYPE
GQVENLELPR HPKEDMGVRV LPFGRELFID AGDFEEVPPA GYKRLIPGGE VRLRGSYVIR
ADEAIKDADG NIVELRCSYD PDTLGKNPEG RKVKGVIHWV PAEGSVECEV RLYDRLFRSA
NPEKAEEGGS FLDNINADSL QVLAGCRAEP SLGQANPEDR FQFEREGYFV ADLKDSRPGK
PVFNRTVTLR DSWGQG
