ID   BMT3_KOMPC              Reviewed;         594 AA.
AC   F2QQ67;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   31-MAY-2011, sequence version 1.
DT   25-MAY-2022, entry version 39.
DE   RecName: Full=Beta-mannosyltransferase 3;
DE            EC=2.4.1.-;
GN   Name=BMT3; OrderedLocusNames=PP7435_Chr1-1431;
OS   Komagataella phaffii (strain ATCC 76273 / CBS 7435 / CECT 11047 / NRRL
OS   Y-11430 / Wegner 21-1) (Yeast) (Pichia pastoris).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Phaffomycetaceae; Komagataella.
OX   NCBI_TaxID=981350;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 76273 / CBS 7435 / CECT 11047 / NRRL Y-11430 / Wegner 21-1;
RX   PubMed=21575661; DOI=10.1016/j.jbiotec.2011.04.014;
RA   Kueberl A., Schneider J., Thallinger G.G., Anderl I., Wibberg D., Hajek T.,
RA   Jaenicke S., Brinkrolf K., Goesmann A., Szczepanowski R., Puehler A.,
RA   Schwab H., Glieder A., Pichler H.;
RT   "High-quality genome sequence of Pichia pastoris CBS7435.";
RL   J. Biotechnol. 154:312-320(2011).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 76273 / CBS 7435 / CECT 11047 / NRRL Y-11430 / Wegner 21-1;
RX   PubMed=27388471; DOI=10.1093/femsyr/fow051;
RA   Valli M., Tatto N.E., Peymann A., Gruber C., Landes N., Ekker H.,
RA   Thallinger G.G., Mattanovich D., Gasser B., Graf A.B.;
RT   "Curation of the genome annotation of Pichia pastoris (Komagataella
RT   phaffii) CBS7435 from gene level to protein function.";
RL   FEMS Yeast Res. 16:0-0(2016).
CC   -!- FUNCTION: Beta-mannosyltransferase involved in cell wall biosynthesis.
CC       {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type II
CC       membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the BMT family. {ECO:0000305}.
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DR   EMBL; FR839628; CCA37545.1; -; Genomic_DNA.
DR   AlphaFoldDB; F2QQ67; -.
DR   CAZy; GT91; Glycosyltransferase Family 91.
DR   EnsemblFungi; CCA37545; CCA37545; PP7435_CHR1-1431.
DR   HOGENOM; CLU_013841_3_0_1; -.
DR   Proteomes; UP000006853; Chromosome 1.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0000030; F:mannosyltransferase activity; IEA:InterPro.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   InterPro; IPR021988; BMT1.
DR   PANTHER; PTHR37989; PTHR37989; 2.
DR   Pfam; PF12141; DUF3589; 2.
PE   3: Inferred from homology;
KW   Cell wall biogenesis/degradation; Coiled coil; Glycoprotein;
KW   Glycosyltransferase; Membrane; Signal-anchor; Transferase; Transmembrane;
KW   Transmembrane helix.
FT   CHAIN           1..594
FT                   /note="Beta-mannosyltransferase 3"
FT                   /id="PRO_0000426103"
FT   TOPO_DOM        1..6
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        7..27
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        28..594
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   REGION          517..564
FT                   /note="Disordered"
FT                   /evidence="ECO:0000256|SAM:MobiDB-lite"
FT   COILED          512..594
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        305
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   594 AA;  68423 MW;  DB09B57D918FEB1A CRC64;
     MRIRSNVLLL STAGALALVW FAVVFSWDDK SIFGIPTPGH AVASAYDSSV TLGTFNDMEV
     DSYVTNIYDN APVLGCYDLS YHGLLKVSPK HEILCDMKFI RARVLETEAY AALKDLEHKK
     LTEEEKIEKH WFTFYGSSVF LPDHDVHYLV RRVVFSGEGK ANRPITSILV AQIYDKNWNE
     LNGHFLNVLN PNTGKLQHHA FPQVLPIAVN WDRNSKYRGQ EDPRVVLRRG RFGPDPLVMF
     NTLTQNNKLR RLFTISPFDQ YKTVMYRTNA FKMQTTEKNW VPFFLKDDQE SVHFVYSFNP
     LRVLNCSLDN GACDVLFELP HDFGMSSELR GATPMLNLPQ AIPMADDKEI WVSFPRTRIS
     DCGCSETMYR PMLMLFVREG TNFFAELLSS SIDFGLEVIP YTGDGLPCSS GQSVLIPNSI
     DNWEVTGSNG EDILSLTFSE ADKSTSVVHI RGLYKYLSEL DGYGGPEAED EHNFQRILSD
     LHFDGKKTIE NFKKVQSCAL DAAKAYCKEY GVTRGEEDRL KNKEKERKIE EKRKKEEERK
     KKEEEKKKKE EEEKKKKEEE EEEEKRLKEL KKKLKELQEE LEKQKDEVKD TKAK