ID   BMT4_KOMPC              Reviewed;         503 AA.
AC   F2QZ45;
DT   16-APR-2014, integrated into UniProtKB/Swiss-Prot.
DT   31-MAY-2011, sequence version 1.
DT   25-MAY-2022, entry version 36.
DE   RecName: Full=Beta-mannosyltransferase 4;
DE            EC=2.4.1.-;
GN   Name=BMT4; OrderedLocusNames=PP7435_Chr4-0509;
OS   Komagataella phaffii (strain ATCC 76273 / CBS 7435 / CECT 11047 / NRRL
OS   Y-11430 / Wegner 21-1) (Yeast) (Pichia pastoris).
OC   Eukaryota; Fungi; Dikarya; Ascomycota; Saccharomycotina; Saccharomycetes;
OC   Saccharomycetales; Phaffomycetaceae; Komagataella.
OX   NCBI_TaxID=981350;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 76273 / CBS 7435 / CECT 11047 / NRRL Y-11430 / Wegner 21-1;
RX   PubMed=21575661; DOI=10.1016/j.jbiotec.2011.04.014;
RA   Kueberl A., Schneider J., Thallinger G.G., Anderl I., Wibberg D., Hajek T.,
RA   Jaenicke S., Brinkrolf K., Goesmann A., Szczepanowski R., Puehler A.,
RA   Schwab H., Glieder A., Pichler H.;
RT   "High-quality genome sequence of Pichia pastoris CBS7435.";
RL   J. Biotechnol. 154:312-320(2011).
RN   [2]
RP   GENOME REANNOTATION.
RC   STRAIN=ATCC 76273 / CBS 7435 / CECT 11047 / NRRL Y-11430 / Wegner 21-1;
RX   PubMed=27388471; DOI=10.1093/femsyr/fow051;
RA   Valli M., Tatto N.E., Peymann A., Gruber C., Landes N., Ekker H.,
RA   Thallinger G.G., Mattanovich D., Gasser B., Graf A.B.;
RT   "Curation of the genome annotation of Pichia pastoris (Komagataella
RT   phaffii) CBS7435 from gene level to protein function.";
RL   FEMS Yeast Res. 16:0-0(2016).
CC   -!- FUNCTION: Beta-mannosyltransferase involved in cell wall biosynthesis.
CC       Responsible for addition of a hexose to the beta-mannose chain (By
CC       similarity). {ECO:0000250}.
CC   -!- SUBCELLULAR LOCATION: Membrane {ECO:0000305}; Single-pass type II
CC       membrane protein {ECO:0000305}.
CC   -!- SIMILARITY: Belongs to the BMT family. {ECO:0000305}.
CC   ---------------------------------------------------------------------------
CC   Copyrighted by the UniProt Consortium, see https://www.uniprot.org/terms
CC   Distributed under the Creative Commons Attribution (CC BY 4.0) License
CC   ---------------------------------------------------------------------------
DR   EMBL; FR839631; CCA40673.1; -; Genomic_DNA.
DR   AlphaFoldDB; F2QZ45; -.
DR   CAZy; GT91; Glycosyltransferase Family 91.
DR   EnsemblFungi; CCA40673; CCA40673; PP7435_CHR4-0509.
DR   HOGENOM; CLU_013841_3_0_1; -.
DR   Proteomes; UP000006853; Chromosome 4.
DR   GO; GO:0016021; C:integral component of membrane; IEA:UniProtKB-KW.
DR   GO; GO:0000030; F:mannosyltransferase activity; IEA:InterPro.
DR   GO; GO:0071555; P:cell wall organization; IEA:UniProtKB-KW.
DR   InterPro; IPR021988; BMT1.
DR   PANTHER; PTHR37989; PTHR37989; 2.
DR   Pfam; PF12141; DUF3589; 2.
PE   3: Inferred from homology;
KW   Cell wall biogenesis/degradation; Glycoprotein; Glycosyltransferase;
KW   Membrane; Signal-anchor; Transferase; Transmembrane; Transmembrane helix.
FT   CHAIN           1..503
FT                   /note="Beta-mannosyltransferase 4"
FT                   /id="PRO_0000426105"
FT   TOPO_DOM        1..24
FT                   /note="Cytoplasmic"
FT                   /evidence="ECO:0000255"
FT   TRANSMEM        25..45
FT                   /note="Helical"
FT                   /evidence="ECO:0000255"
FT   TOPO_DOM        46..503
FT                   /note="Extracellular"
FT                   /evidence="ECO:0000255"
FT   CARBOHYD        468
FT                   /note="N-linked (GlcNAc...) asparagine"
FT                   /evidence="ECO:0000255"
SQ   SEQUENCE   503 AA;  56665 MW;  CD914139E9E988A5 CRC64;
     MKLDTQQISH LLSRQMYHLA PRKKLLIWGG SLGFVLLLLI VASSHQRIRS TILHRTPIST
     LPVISQEVIT ADYHPTLLTG FIPTDSDDSD CADFSPSGVI YSTDKLVLHD SLKDIRDSLL
     KTQYKDLVTL EDEEKMNIDD ILKRWYTLSG SSVWIPGMKA HLVVSRVMYL GTNGRSDPLV
     SFVRVQLFDP DFNELKDIAL KFSDKPDGTV IFPYILPVDI PREGSRWLGP EDAKIAVNPE
     TPDDPIVIFN MQNSVNRAMY GFYPFRPENK QVLFSIKDEE PRKKEKNWTP FFVPGSPTTV
     NFVYDLQKLT ILKCSIITGI CEKEFVSGDD GQNHGIGIFR GGSNLVPFPT SFTDKDVWVG
     FPKTHMESCG CSSHIYRPYL MVLVRKGDFY YKAFVSTPLD FGIDVRSWES AESTSCQTAK
     NVLAVNSISN WDLLDDGLDK DYMTITLSEA DVVNSVLRVR GIAKFVDNLT MDDGSTTLST
     SNKIDECATT GSKQYCQRYG ELH