ID   SYR_ALKOO               Reviewed;         587 AA.
AC   A8MKD9;
DT   28-JUL-2009, integrated into UniProtKB/Swiss-Prot.
DT   04-DEC-2007, sequence version 1.
DT   25-MAY-2022, entry version 75.
DE   RecName: Full=Arginine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00123};
DE            EC=6.1.1.19 {ECO:0000255|HAMAP-Rule:MF_00123};
DE   AltName: Full=Arginyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00123};
DE            Short=ArgRS {ECO:0000255|HAMAP-Rule:MF_00123};
GN   Name=argS {ECO:0000255|HAMAP-Rule:MF_00123}; OrderedLocusNames=Clos_2740;
OS   Alkaliphilus oremlandii (strain OhILAs) (Clostridium oremlandii (strain
OS   OhILAs)).
OC   Bacteria; Firmicutes; Clostridia; Eubacteriales; Clostridiaceae;
OC   Alkaliphilus.
OX   NCBI_TaxID=350688;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=OhILAs;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C.,
RA   Glavina del Rio T., Hammon N., Israni S., Dalin E., Tice H., Pitluck S.,
RA   Chain P., Malfatti S., Shin M., Vergez L., Schmutz J., Larimer F., Land M.,
RA   Hauser L., Kyrpides N., Mikhailova N., Stolz J.F., Dawson A., Fisher E.,
RA   Crable B., Perera E., Lisak J., Ranganathan M., Basu P., Richardson P.;
RT   "Complete genome of Alkaliphilus oremlandii OhILAs.";
RL   Submitted (OCT-2007) to the EMBL/GenBank/DDBJ databases.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-
CC         tRNA(Arg); Xref=Rhea:RHEA:20301, Rhea:RHEA-COMP:9658, Rhea:RHEA-
CC         COMP:9673, ChEBI:CHEBI:30616, ChEBI:CHEBI:32682, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78513, ChEBI:CHEBI:456215;
CC         EC=6.1.1.19; Evidence={ECO:0000255|HAMAP-Rule:MF_00123};
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00123}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00123}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00123}.
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DR   EMBL; CP000853; ABW20271.1; -; Genomic_DNA.
DR   RefSeq; WP_012160578.1; NC_009922.1.
DR   AlphaFoldDB; A8MKD9; -.
DR   SMR; A8MKD9; -.
DR   STRING; 350688.Clos_2740; -.
DR   EnsemblBacteria; ABW20271; ABW20271; Clos_2740.
DR   KEGG; aoe:Clos_2740; -.
DR   eggNOG; COG0018; Bacteria.
DR   HOGENOM; CLU_006406_5_1_9; -.
DR   OMA; HHIGDWG; -.
DR   OrthoDB; 1146366at2; -.
DR   Proteomes; UP000000269; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.30.1360.70; -; 1.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_00123; Arg_tRNA_synth; 1.
DR   InterPro; IPR001278; Arg-tRNA-ligase.
DR   InterPro; IPR005148; Arg-tRNA-synth_N.
DR   InterPro; IPR036695; Arg-tRNA-synth_N_sf.
DR   InterPro; IPR035684; ArgRS_core.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   PANTHER; PTHR11956; PTHR11956; 1.
DR   Pfam; PF03485; Arg_tRNA_synt_N; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF00750; tRNA-synt_1d; 1.
DR   PRINTS; PR01038; TRNASYNTHARG.
DR   SMART; SM01016; Arg_tRNA_synt_N; 1.
DR   SMART; SM00836; DALR_1; 1.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF55190; SSF55190; 1.
DR   TIGRFAMs; TIGR00456; argS; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..587
FT                   /note="Arginine--tRNA ligase"
FT                   /id="PRO_1000198869"
FT   MOTIF           123..133
FT                   /note="'HIGH' region"
SQ   SEQUENCE   587 AA;  66303 MW;  49E70A6DFD7C614E CRC64;
     MELLIKQISA FVEDVFEQKG YDKKYGMVTV SDRPDLCQFQ CNGALPAAKE YKKAPIQIAN
     DLLPALKNVE IFEEITIAGP GFININVKDS FLAAYVNEMY GSEKLGLEEP AEPQTIIIDY
     GGANVAKPLH VGHLRAAIIG ESLKRISRFV GHKVIGDVHL GDWGLQMGMV ISELRRRSPH
     LPYFDESYEG EYPAEAPFTI DELEDIYPAA SKLAKSDAAA MEEAQQATAE LQKGRRGYMA
     LWQHILNVSV ADLKKGYGNL NVDFDLWNGE SDCQKYIDVM VDYLKKNNYT SFSDGLLIVD
     VAKETDNHVI PPFIVLKTDG SSLYSTTDLA TIWERVQDYD PDQIIYVVDK RQELHFEQVF
     RCAKKTKIAG EDLTLKFLGF GTMNGKDGKP FKTREGGVMR LQDLIQIIKD AVYNKLQENE
     AIDPSEVDEI SRKVGLAALK YGDLSNQITK DYVFDIDRFA SFEGNTGPYI LYTIVRIKSI
     LRRIAAEGFQ ASDKIGVPHS DVERSLLLKL SRFNETVEFS FVNHSPNRIC DYIYDLANTF
     NKFYHDTKII SETEFEKKSS WINLITLTMN VLETCLELLG IEAPEKM