SYR_CAMJE
ID SYR_CAMJE Reviewed; 530 AA.
AC Q9PNC0; Q0P981;
DT 27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-2000, sequence version 1.
DT 03-AUG-2022, entry version 116.
DE RecName: Full=Arginine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00123};
DE EC=6.1.1.19 {ECO:0000255|HAMAP-Rule:MF_00123};
DE AltName: Full=Arginyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00123};
DE Short=ArgRS {ECO:0000255|HAMAP-Rule:MF_00123};
GN Name=argS {ECO:0000255|HAMAP-Rule:MF_00123}; OrderedLocusNames=Cj1175c;
OS Campylobacter jejuni subsp. jejuni serotype O:2 (strain ATCC 700819 / NCTC
OS 11168).
OC Bacteria; Proteobacteria; Epsilonproteobacteria; Campylobacterales;
OC Campylobacteraceae; Campylobacter.
OX NCBI_TaxID=192222;
RN [1]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=ATCC 700819 / NCTC 11168;
RX PubMed=10688204; DOI=10.1038/35001088;
RA Parkhill J., Wren B.W., Mungall K.L., Ketley J.M., Churcher C.M.,
RA Basham D., Chillingworth T., Davies R.M., Feltwell T., Holroyd S.,
RA Jagels K., Karlyshev A.V., Moule S., Pallen M.J., Penn C.W., Quail M.A.,
RA Rajandream M.A., Rutherford K.M., van Vliet A.H.M., Whitehead S.,
RA Barrell B.G.;
RT "The genome sequence of the food-borne pathogen Campylobacter jejuni
RT reveals hypervariable sequences.";
RL Nature 403:665-668(2000).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-
CC tRNA(Arg); Xref=Rhea:RHEA:20301, Rhea:RHEA-COMP:9658, Rhea:RHEA-
CC COMP:9673, ChEBI:CHEBI:30616, ChEBI:CHEBI:32682, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78513, ChEBI:CHEBI:456215;
CC EC=6.1.1.19; Evidence={ECO:0000255|HAMAP-Rule:MF_00123};
CC -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00123}.
CC -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00123}.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000255|HAMAP-Rule:MF_00123}.
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DR EMBL; AL111168; CAL35290.1; -; Genomic_DNA.
DR PIR; A81323; A81323.
DR RefSeq; WP_010891916.1; NC_002163.1.
DR RefSeq; YP_002344566.1; NC_002163.1.
DR PDB; 3FNR; X-ray; 2.20 A; A=77-530.
DR PDBsum; 3FNR; -.
DR AlphaFoldDB; Q9PNC0; -.
DR SMR; Q9PNC0; -.
DR IntAct; Q9PNC0; 3.
DR STRING; 192222.Cj1175c; -.
DR PaxDb; Q9PNC0; -.
DR PRIDE; Q9PNC0; -.
DR DNASU; 905465; -.
DR EnsemblBacteria; CAL35290; CAL35290; Cj1175c.
DR GeneID; 905465; -.
DR KEGG; cje:Cj1175c; -.
DR PATRIC; fig|192222.6.peg.1156; -.
DR eggNOG; COG0018; Bacteria.
DR HOGENOM; CLU_006406_0_1_7; -.
DR OMA; NKPLHLG; -.
DR EvolutionaryTrace; Q9PNC0; -.
DR Proteomes; UP000000799; Chromosome.
DR GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR CDD; cd00671; ArgRS_core; 1.
DR Gene3D; 3.30.1360.70; -; 1.
DR Gene3D; 3.40.50.620; -; 1.
DR HAMAP; MF_00123; Arg_tRNA_synth; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR001278; Arg-tRNA-ligase.
DR InterPro; IPR005148; Arg-tRNA-synth_N.
DR InterPro; IPR036695; Arg-tRNA-synth_N_sf.
DR InterPro; IPR035684; ArgRS_core.
DR InterPro; IPR008909; DALR_anticod-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR PANTHER; PTHR11956; PTHR11956; 1.
DR Pfam; PF03485; Arg_tRNA_synt_N; 1.
DR Pfam; PF05746; DALR_1; 1.
DR Pfam; PF00750; tRNA-synt_1d; 1.
DR PRINTS; PR01038; TRNASYNTHARG.
DR SMART; SM01016; Arg_tRNA_synt_N; 1.
DR SMART; SM00836; DALR_1; 1.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF55190; SSF55190; 1.
DR TIGRFAMs; TIGR00456; argS; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT CHAIN 1..530
FT /note="Arginine--tRNA ligase"
FT /id="PRO_0000242001"
FT MOTIF 113..123
FT /note="'HIGH' region"
FT HELIX 79..91
FT /evidence="ECO:0007829|PDB:3FNR"
FT HELIX 93..95
FT /evidence="ECO:0007829|PDB:3FNR"
FT STRAND 105..109
FT /evidence="ECO:0007829|PDB:3FNR"
FT STRAND 115..118
FT /evidence="ECO:0007829|PDB:3FNR"
FT HELIX 121..123
FT /evidence="ECO:0007829|PDB:3FNR"
FT HELIX 124..139
FT /evidence="ECO:0007829|PDB:3FNR"
FT STRAND 143..150
FT /evidence="ECO:0007829|PDB:3FNR"
FT HELIX 154..171
FT /evidence="ECO:0007829|PDB:3FNR"
FT HELIX 187..200
FT /evidence="ECO:0007829|PDB:3FNR"
FT HELIX 202..204
FT /evidence="ECO:0007829|PDB:3FNR"
FT HELIX 207..209
FT /evidence="ECO:0007829|PDB:3FNR"
FT HELIX 210..231
FT /evidence="ECO:0007829|PDB:3FNR"
FT STRAND 239..241
FT /evidence="ECO:0007829|PDB:3FNR"
FT HELIX 242..245
FT /evidence="ECO:0007829|PDB:3FNR"
FT HELIX 249..258
FT /evidence="ECO:0007829|PDB:3FNR"
FT STRAND 262..265
FT /evidence="ECO:0007829|PDB:3FNR"
FT STRAND 268..271
FT /evidence="ECO:0007829|PDB:3FNR"
FT HELIX 273..276
FT /evidence="ECO:0007829|PDB:3FNR"
FT STRAND 282..285
FT /evidence="ECO:0007829|PDB:3FNR"
FT HELIX 293..305
FT /evidence="ECO:0007829|PDB:3FNR"
FT STRAND 310..317
FT /evidence="ECO:0007829|PDB:3FNR"
FT HELIX 318..323
FT /evidence="ECO:0007829|PDB:3FNR"
FT HELIX 324..332
FT /evidence="ECO:0007829|PDB:3FNR"
FT TURN 333..335
FT /evidence="ECO:0007829|PDB:3FNR"
FT HELIX 338..340
FT /evidence="ECO:0007829|PDB:3FNR"
FT STRAND 341..346
FT /evidence="ECO:0007829|PDB:3FNR"
FT STRAND 349..353
FT /evidence="ECO:0007829|PDB:3FNR"
FT HELIX 370..377
FT /evidence="ECO:0007829|PDB:3FNR"
FT HELIX 379..385
FT /evidence="ECO:0007829|PDB:3FNR"
FT STRAND 395..398
FT /evidence="ECO:0007829|PDB:3FNR"
FT HELIX 399..403
FT /evidence="ECO:0007829|PDB:3FNR"
FT HELIX 410..427
FT /evidence="ECO:0007829|PDB:3FNR"
FT TURN 432..434
FT /evidence="ECO:0007829|PDB:3FNR"
FT HELIX 445..455
FT /evidence="ECO:0007829|PDB:3FNR"
FT HELIX 457..467
FT /evidence="ECO:0007829|PDB:3FNR"
FT HELIX 472..490
FT /evidence="ECO:0007829|PDB:3FNR"
FT HELIX 499..519
FT /evidence="ECO:0007829|PDB:3FNR"
SQ SEQUENCE 530 AA; 60190 MW; D2C9B35AF857B2B8 CRC64;
MKSIIFNEIK KILECDFALE NPKDKNLAHF ATPLAFSLAK ELKKSPMLIA SDLASKFQNH
DCFESVEAVN GYLNFRISKT FLNELANQAL TNPNDFTKGE KKQESFLLEY VSANPTGPLH
IGHARGAVFG DTLTRLARHL GYKFNTEYYV NDAGNQIYLL GLSILLSVKE SILHENVEYP
EQYYKGEYIV DLAKEAFEKF GKEFFSEENI PSLADWAKDK MLVLIKQNLE QAKIKIDSYV
SERSYYDALN ATLESLKEHK GIYEQEGKIW LASSQKGDEK DRVIIREDGR GTYLAADIVY
HKDKMSRGYG KCINIWGADH HGYIPRMKAA MEFLGFDSNN LEIILAQMVS LLKDGEPYKM
SKRAGNFILM SDVVDEIGSD ALRYIFLSKK CDTHLEFDIS DLQKEDSSNP VYYINYAHAR
IHQVFAKAGK KIDDVMKADL QSLNQDGVNL LFEALNLKAV LNDAFEARAL QKIPDYLKNL
AANFHKFYNE NKVVGSANEN DLLKLFSLVA LSIKTAFSLM GIEAKNKMEH
