BN3D2_DROME
ID BN3D2_DROME Reviewed; 300 AA.
AC Q9VNH1; A0ANN6; A0ANP4; C0MJ20; C0MJ21;
DT 29-MAY-2007, integrated into UniProtKB/Swiss-Prot.
DT 01-MAR-2001, sequence version 2.
DT 03-AUG-2022, entry version 128.
DE RecName: Full=Probable RNA methyltransferase CG1239;
DE EC=2.1.1.- {ECO:0000250|UniProtKB:Q7Z5W3};
GN ORFNames=CG1239;
OS Drosophila melanogaster (Fruit fly).
OC Eukaryota; Metazoa; Ecdysozoa; Arthropoda; Hexapoda; Insecta; Pterygota;
OC Neoptera; Endopterygota; Diptera; Brachycera; Muscomorpha; Ephydroidea;
OC Drosophilidae; Drosophila; Sophophora.
OX NCBI_TaxID=7227;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=ZBMEL131, ZBMEL145, ZBMEL157, ZBMEL186, ZBMEL191, ZBMEL229,
RC ZBMEL377, ZBMEL384, ZBMEL398, ZBMEL82, ZBMEL84, and ZBMEL95;
RX PubMed=16951084; DOI=10.1534/genetics.106.058008;
RA Proeschel M., Zhang Z., Parsch J.;
RT "Widespread adaptive evolution of Drosophila genes with sex-biased
RT expression.";
RL Genetics 174:893-900(2006).
RN [2]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA].
RC STRAIN=MEL01, MEL11, MEL12, MEL13, MEL14, MEL15, MEL16, MEL17, MEL18,
RC MEL19, and MEL20;
RX PubMed=19126864; DOI=10.1093/molbev/msn297;
RA Parsch J., Zhang Z., Baines J.F.;
RT "The influence of demography and weak selection on the McDonald-Kreitman
RT test: an empirical study in Drosophila.";
RL Mol. Biol. Evol. 26:691-698(2009).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=Berkeley;
RX PubMed=10731132; DOI=10.1126/science.287.5461.2185;
RA Adams M.D., Celniker S.E., Holt R.A., Evans C.A., Gocayne J.D.,
RA Amanatides P.G., Scherer S.E., Li P.W., Hoskins R.A., Galle R.F.,
RA George R.A., Lewis S.E., Richards S., Ashburner M., Henderson S.N.,
RA Sutton G.G., Wortman J.R., Yandell M.D., Zhang Q., Chen L.X., Brandon R.C.,
RA Rogers Y.-H.C., Blazej R.G., Champe M., Pfeiffer B.D., Wan K.H., Doyle C.,
RA Baxter E.G., Helt G., Nelson C.R., Miklos G.L.G., Abril J.F., Agbayani A.,
RA An H.-J., Andrews-Pfannkoch C., Baldwin D., Ballew R.M., Basu A.,
RA Baxendale J., Bayraktaroglu L., Beasley E.M., Beeson K.Y., Benos P.V.,
RA Berman B.P., Bhandari D., Bolshakov S., Borkova D., Botchan M.R., Bouck J.,
RA Brokstein P., Brottier P., Burtis K.C., Busam D.A., Butler H., Cadieu E.,
RA Center A., Chandra I., Cherry J.M., Cawley S., Dahlke C., Davenport L.B.,
RA Davies P., de Pablos B., Delcher A., Deng Z., Mays A.D., Dew I.,
RA Dietz S.M., Dodson K., Doup L.E., Downes M., Dugan-Rocha S., Dunkov B.C.,
RA Dunn P., Durbin K.J., Evangelista C.C., Ferraz C., Ferriera S.,
RA Fleischmann W., Fosler C., Gabrielian A.E., Garg N.S., Gelbart W.M.,
RA Glasser K., Glodek A., Gong F., Gorrell J.H., Gu Z., Guan P., Harris M.,
RA Harris N.L., Harvey D.A., Heiman T.J., Hernandez J.R., Houck J., Hostin D.,
RA Houston K.A., Howland T.J., Wei M.-H., Ibegwam C., Jalali M., Kalush F.,
RA Karpen G.H., Ke Z., Kennison J.A., Ketchum K.A., Kimmel B.E., Kodira C.D.,
RA Kraft C.L., Kravitz S., Kulp D., Lai Z., Lasko P., Lei Y., Levitsky A.A.,
RA Li J.H., Li Z., Liang Y., Lin X., Liu X., Mattei B., McIntosh T.C.,
RA McLeod M.P., McPherson D., Merkulov G., Milshina N.V., Mobarry C.,
RA Morris J., Moshrefi A., Mount S.M., Moy M., Murphy B., Murphy L.,
RA Muzny D.M., Nelson D.L., Nelson D.R., Nelson K.A., Nixon K., Nusskern D.R.,
RA Pacleb J.M., Palazzolo M., Pittman G.S., Pan S., Pollard J., Puri V.,
RA Reese M.G., Reinert K., Remington K., Saunders R.D.C., Scheeler F.,
RA Shen H., Shue B.C., Siden-Kiamos I., Simpson M., Skupski M.P., Smith T.J.,
RA Spier E., Spradling A.C., Stapleton M., Strong R., Sun E., Svirskas R.,
RA Tector C., Turner R., Venter E., Wang A.H., Wang X., Wang Z.-Y.,
RA Wassarman D.A., Weinstock G.M., Weissenbach J., Williams S.M., Woodage T.,
RA Worley K.C., Wu D., Yang S., Yao Q.A., Ye J., Yeh R.-F., Zaveri J.S.,
RA Zhan M., Zhang G., Zhao Q., Zheng L., Zheng X.H., Zhong F.N., Zhong W.,
RA Zhou X., Zhu S.C., Zhu X., Smith H.O., Gibbs R.A., Myers E.W., Rubin G.M.,
RA Venter J.C.;
RT "The genome sequence of Drosophila melanogaster.";
RL Science 287:2185-2195(2000).
RN [4]
RP GENOME REANNOTATION.
RC STRAIN=Berkeley;
RX PubMed=12537572; DOI=10.1186/gb-2002-3-12-research0083;
RA Misra S., Crosby M.A., Mungall C.J., Matthews B.B., Campbell K.S.,
RA Hradecky P., Huang Y., Kaminker J.S., Millburn G.H., Prochnik S.E.,
RA Smith C.D., Tupy J.L., Whitfield E.J., Bayraktaroglu L., Berman B.P.,
RA Bettencourt B.R., Celniker S.E., de Grey A.D.N.J., Drysdale R.A.,
RA Harris N.L., Richter J., Russo S., Schroeder A.J., Shu S.Q., Stapleton M.,
RA Yamada C., Ashburner M., Gelbart W.M., Rubin G.M., Lewis S.E.;
RT "Annotation of the Drosophila melanogaster euchromatic genome: a systematic
RT review.";
RL Genome Biol. 3:RESEARCH0083.1-RESEARCH0083.22(2002).
RN [5]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
RC STRAIN=Berkeley; TISSUE=Larva, and Pupae;
RA Stapleton M., Brokstein P., Hong L., Agbayani A., Carlson J.W., Champe M.,
RA Chavez C., Dorsett V., Dresnek D., Farfan D., Frise E., George R.A.,
RA Gonzalez M., Guarin H., Kronmiller B., Li P.W., Liao G., Miranda A.,
RA Mungall C.J., Nunoo J., Pacleb J.M., Paragas V., Park S., Patel S.,
RA Phouanenavong S., Wan K.H., Yu C., Lewis S.E., Rubin G.M., Celniker S.E.;
RL Submitted (JAN-2003) to the EMBL/GenBank/DDBJ databases.
RN [6]
RP DISRUPTION PHENOTYPE.
RX PubMed=31145769; DOI=10.1371/journal.pone.0217603;
RA Zhu L., Liao S.E., Ai Y., Fukunaga R.;
RT "RNA methyltransferase BCDIN3D is crucial for female fertility and miRNA
RT and mRNA profiles in Drosophila ovaries.";
RL PLoS ONE 14:e0217603-e0217603(2019).
CC -!- FUNCTION: Probable RNA methyltransferase.
CC {ECO:0000250|UniProtKB:Q7Z5W3}.
CC -!- DISRUPTION PHENOTYPE: Embryonic lethality (PubMed:31145769).
CC Conditional knockdown in ovary leads to female sterility, possibly
CC caused by dysregulation of miRNAs and mRNAsso (PubMed:31145769).
CC {ECO:0000269|PubMed:31145769}.
CC -!- SIMILARITY: Belongs to the methyltransferase superfamily.
CC {ECO:0000305}.
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DR EMBL; AM294273; CAL26177.1; -; Genomic_DNA.
DR EMBL; AM294274; CAL26178.1; -; Genomic_DNA.
DR EMBL; AM294275; CAL26179.1; -; Genomic_DNA.
DR EMBL; AM294276; CAL26180.1; -; Genomic_DNA.
DR EMBL; AM294277; CAL26181.1; -; Genomic_DNA.
DR EMBL; AM294278; CAL26182.1; -; Genomic_DNA.
DR EMBL; AM294279; CAL26183.1; -; Genomic_DNA.
DR EMBL; AM294280; CAL26184.1; -; Genomic_DNA.
DR EMBL; AM294281; CAL26185.1; -; Genomic_DNA.
DR EMBL; AM294282; CAL26186.1; -; Genomic_DNA.
DR EMBL; AM294283; CAL26187.1; -; Genomic_DNA.
DR EMBL; AM294284; CAL26188.1; -; Genomic_DNA.
DR EMBL; AE014297; AAF51965.2; -; Genomic_DNA.
DR EMBL; FM245342; CAR93268.1; -; Genomic_DNA.
DR EMBL; FM245343; CAR93269.1; -; Genomic_DNA.
DR EMBL; FM245344; CAR93270.1; -; Genomic_DNA.
DR EMBL; FM245345; CAR93271.1; -; Genomic_DNA.
DR EMBL; FM245346; CAR93272.1; -; Genomic_DNA.
DR EMBL; FM245347; CAR93273.1; -; Genomic_DNA.
DR EMBL; FM245348; CAR93274.1; -; Genomic_DNA.
DR EMBL; FM245349; CAR93275.1; -; Genomic_DNA.
DR EMBL; FM245350; CAR93276.1; -; Genomic_DNA.
DR EMBL; FM245351; CAR93277.1; -; Genomic_DNA.
DR EMBL; FM245352; CAR93278.1; -; Genomic_DNA.
DR EMBL; AY061533; AAL29081.1; -; mRNA.
DR RefSeq; NP_001287185.1; NM_001300256.1.
DR RefSeq; NP_001287186.1; NM_001300257.1.
DR RefSeq; NP_649577.1; NM_141320.3.
DR AlphaFoldDB; Q9VNH1; -.
DR SMR; Q9VNH1; -.
DR BioGRID; 65911; 1.
DR IntAct; Q9VNH1; 6.
DR STRING; 7227.FBpp0078369; -.
DR PaxDb; Q9VNH1; -.
DR DNASU; 40706; -.
DR EnsemblMetazoa; FBtr0078720; FBpp0078369; FBgn0037368.
DR EnsemblMetazoa; FBtr0343988; FBpp0310437; FBgn0037368.
DR EnsemblMetazoa; FBtr0343989; FBpp0310438; FBgn0037368.
DR GeneID; 40706; -.
DR KEGG; dme:Dmel_CG1239; -.
DR UCSC; CG1239-RA; d. melanogaster.
DR FlyBase; FBgn0037368; CG1239.
DR VEuPathDB; VectorBase:FBgn0037368; -.
DR eggNOG; KOG2899; Eukaryota.
DR GeneTree; ENSGT00940000153993; -.
DR HOGENOM; CLU_004729_0_0_1; -.
DR InParanoid; Q9VNH1; -.
DR OMA; ELFRGKQ; -.
DR OrthoDB; 1185441at2759; -.
DR PhylomeDB; Q9VNH1; -.
DR BioGRID-ORCS; 40706; 0 hits in 1 CRISPR screen.
DR GenomeRNAi; 40706; -.
DR PRO; PR:Q9VNH1; -.
DR Proteomes; UP000000803; Chromosome 3R.
DR Bgee; FBgn0037368; Expressed in adult abdomen and 24 other tissues.
DR ExpressionAtlas; Q9VNH1; baseline and differential.
DR Genevisible; Q9VNH1; DM.
DR GO; GO:0008171; F:O-methyltransferase activity; IBA:GO_Central.
DR GO; GO:0008173; F:RNA methyltransferase activity; IBA:GO_Central.
DR GO; GO:0017069; F:snRNA binding; IBA:GO_Central.
DR GO; GO:0040031; P:snRNA modification; IBA:GO_Central.
DR Gene3D; 3.40.50.150; -; 1.
DR InterPro; IPR039772; Bin3-like.
DR InterPro; IPR010675; Bin3_C.
DR InterPro; IPR024160; BIN3_SAM-bd_dom.
DR InterPro; IPR029063; SAM-dependent_MTases_sf.
DR PANTHER; PTHR12315; PTHR12315; 2.
DR Pfam; PF06859; Bin3; 1.
DR SUPFAM; SSF53335; SSF53335; 1.
DR PROSITE; PS51515; BIN3_SAM; 1.
PE 2: Evidence at transcript level;
KW Methyltransferase; Reference proteome; S-adenosyl-L-methionine;
KW Transferase.
FT CHAIN 1..300
FT /note="Probable RNA methyltransferase CG1239"
FT /id="PRO_0000289270"
FT DOMAIN 95..300
FT /note="Bin3-type SAM"
FT /evidence="ECO:0000255|PROSITE-ProRule:PRU00848"
FT REGION 1..21
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT REGION 34..68
FT /note="Disordered"
FT /evidence="ECO:0000256|SAM:MobiDB-lite"
FT BINDING 119
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000250|UniProtKB:Q7Z5W3"
FT BINDING 140
FT /ligand="S-adenosyl-L-methionine"
FT /ligand_id="ChEBI:CHEBI:59789"
FT /evidence="ECO:0000250|UniProtKB:Q7Z5W3"
FT VARIANT 16
FT /note="A -> P (in strain: ZBMEL377)"
FT VARIANT 298
FT /note="T -> K (in strain: ZBMEL84, ZBMEL95, ZBMEL131 and
FT ZBMEL157)"
SQ SEQUENCE 300 AA; 34724 MW; D8A0B151D40EA96B CRC64;
MDLENNNNTP LTGKQAEKCA KKRKCVITLD EKQVESKRLK KEESNVEATS RPPAQSPKKR
LHLNGKPMQN KDLNFKYGNY KHYYGKRILN KDFHDIRLDV LGTQPDLFRN KQLLDIGCNS
GHLSIQIARK FEVKSLVGLD IDRGLINDAQ KTVSHLKRHA TPGQGIPHIQ FVHGNYVLED
DVLLEIERPQ FDVILCLSVT KWIHLNFCDS GLKQAFRRMY LQLRPGGKLI LEPQSFDGYK
RRKKLSEQIR DNYNAIKFRP DHFTEYLLSP EVGFAEMKLM GIPEHCKVGF KRPIQIFTKS
