SYR_ECOLI
ID SYR_ECOLI Reviewed; 577 AA.
AC P11875;
DT 01-OCT-1989, integrated into UniProtKB/Swiss-Prot.
DT 01-OCT-1989, sequence version 1.
DT 03-AUG-2022, entry version 184.
DE RecName: Full=Arginine--tRNA ligase;
DE EC=6.1.1.19;
DE AltName: Full=Arginyl-tRNA synthetase;
DE Short=ArgRS;
GN Name=argS; OrderedLocusNames=b1876, JW1865;
OS Escherichia coli (strain K12).
OC Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales;
OC Enterobacteriaceae; Escherichia.
OX NCBI_TaxID=83333;
RN [1]
RP NUCLEOTIDE SEQUENCE [GENOMIC DNA], AND PROTEIN SEQUENCE OF 1-19.
RC STRAIN=K12;
RX PubMed=2668891; DOI=10.1093/nar/17.14.5725;
RA Eriani G., Dirheimer G., Gangloff J.;
RT "Isolation and characterization of the gene coding for Escherichia coli
RT arginyl-tRNA synthetase.";
RL Nucleic Acids Res. 17:5725-5736(1989).
RN [2]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=9097040; DOI=10.1093/dnares/3.6.379;
RA Itoh T., Aiba H., Baba T., Fujita K., Hayashi K., Inada T., Isono K.,
RA Kasai H., Kimura S., Kitakawa M., Kitagawa M., Makino K., Miki T.,
RA Mizobuchi K., Mori H., Mori T., Motomura K., Nakade S., Nakamura Y.,
RA Nashimoto H., Nishio Y., Oshima T., Saito N., Sampei G., Seki Y.,
RA Sivasundaram S., Tagami H., Takeda J., Takemoto K., Wada C., Yamamoto Y.,
RA Horiuchi T.;
RT "A 460-kb DNA sequence of the Escherichia coli K-12 genome corresponding to
RT the 40.1-50.0 min region on the linkage map.";
RL DNA Res. 3:379-392(1996).
RN [3]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / MG1655 / ATCC 47076;
RX PubMed=9278503; DOI=10.1126/science.277.5331.1453;
RA Blattner F.R., Plunkett G. III, Bloch C.A., Perna N.T., Burland V.,
RA Riley M., Collado-Vides J., Glasner J.D., Rode C.K., Mayhew G.F.,
RA Gregor J., Davis N.W., Kirkpatrick H.A., Goeden M.A., Rose D.J., Mau B.,
RA Shao Y.;
RT "The complete genome sequence of Escherichia coli K-12.";
RL Science 277:1453-1462(1997).
RN [4]
RP NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC STRAIN=K12 / W3110 / ATCC 27325 / DSM 5911;
RX PubMed=16738553; DOI=10.1038/msb4100049;
RA Hayashi K., Morooka N., Yamamoto Y., Fujita K., Isono K., Choi S.,
RA Ohtsubo E., Baba T., Wanner B.L., Mori H., Horiuchi T.;
RT "Highly accurate genome sequences of Escherichia coli K-12 strains MG1655
RT and W3110.";
RL Mol. Syst. Biol. 2:E1-E5(2006).
RN [5]
RP IDENTIFICATION BY 2D-GEL.
RX PubMed=9298644; DOI=10.1002/elps.1150180805;
RA VanBogelen R.A., Abshire K.Z., Moldover B., Olson E.R., Neidhardt F.C.;
RT "Escherichia coli proteome analysis using the gene-protein database.";
RL Electrophoresis 18:1243-1251(1997).
CC -!- CATALYTIC ACTIVITY:
CC Reaction=ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-
CC tRNA(Arg); Xref=Rhea:RHEA:20301, Rhea:RHEA-COMP:9658, Rhea:RHEA-
CC COMP:9673, ChEBI:CHEBI:30616, ChEBI:CHEBI:32682, ChEBI:CHEBI:33019,
CC ChEBI:CHEBI:78442, ChEBI:CHEBI:78513, ChEBI:CHEBI:456215;
CC EC=6.1.1.19;
CC -!- SUBUNIT: Monomer.
CC -!- SUBCELLULAR LOCATION: Cytoplasm.
CC -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC {ECO:0000305}.
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DR EMBL; X15320; CAA33384.1; -; Genomic_DNA.
DR EMBL; U00096; AAC74946.1; -; Genomic_DNA.
DR EMBL; AP009048; BAA15686.1; -; Genomic_DNA.
DR PIR; JS0267; SYECRT.
DR RefSeq; NP_416390.1; NC_000913.3.
DR RefSeq; WP_001025322.1; NZ_SSZK01000001.1.
DR PDB; 4OBY; X-ray; 2.57 A; A=1-577.
DR PDB; 5B63; X-ray; 3.00 A; A/C=1-577.
DR PDB; 5YYM; X-ray; 2.20 A; A/B=1-577.
DR PDB; 5YYN; X-ray; 3.00 A; A/C=1-577.
DR PDBsum; 4OBY; -.
DR PDBsum; 5B63; -.
DR PDBsum; 5YYM; -.
DR PDBsum; 5YYN; -.
DR AlphaFoldDB; P11875; -.
DR SMR; P11875; -.
DR BioGRID; 4260368; 43.
DR DIP; DIP-9147N; -.
DR IntAct; P11875; 10.
DR STRING; 511145.b1876; -.
DR SWISS-2DPAGE; P11875; -.
DR jPOST; P11875; -.
DR PaxDb; P11875; -.
DR PRIDE; P11875; -.
DR EnsemblBacteria; AAC74946; AAC74946; b1876.
DR EnsemblBacteria; BAA15686; BAA15686; BAA15686.
DR GeneID; 58462343; -.
DR GeneID; 946452; -.
DR KEGG; ecj:JW1865; -.
DR KEGG; eco:b1876; -.
DR PATRIC; fig|1411691.4.peg.372; -.
DR EchoBASE; EB0069; -.
DR eggNOG; COG0018; Bacteria.
DR HOGENOM; CLU_006406_5_1_6; -.
DR InParanoid; P11875; -.
DR OMA; NKPLHLG; -.
DR PhylomeDB; P11875; -.
DR BioCyc; EcoCyc:ARGS-MON; -.
DR BioCyc; MetaCyc:ARGS-MON; -.
DR BRENDA; 6.1.1.19; 2026.
DR SABIO-RK; P11875; -.
DR PRO; PR:P11875; -.
DR Proteomes; UP000000318; Chromosome.
DR Proteomes; UP000000625; Chromosome.
DR GO; GO:0005829; C:cytosol; IDA:EcoCyc.
DR GO; GO:0004814; F:arginine-tRNA ligase activity; IDA:EcoCyc.
DR GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR GO; GO:0006420; P:arginyl-tRNA aminoacylation; IDA:EcoCyc.
DR CDD; cd00671; ArgRS_core; 1.
DR Gene3D; 3.30.1360.70; -; 1.
DR Gene3D; 3.40.50.620; -; 1.
DR HAMAP; MF_00123; Arg_tRNA_synth; 1.
DR InterPro; IPR001412; aa-tRNA-synth_I_CS.
DR InterPro; IPR001278; Arg-tRNA-ligase.
DR InterPro; IPR005148; Arg-tRNA-synth_N.
DR InterPro; IPR036695; Arg-tRNA-synth_N_sf.
DR InterPro; IPR035684; ArgRS_core.
DR InterPro; IPR008909; DALR_anticod-bd.
DR InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR PANTHER; PTHR11956; PTHR11956; 1.
DR Pfam; PF03485; Arg_tRNA_synt_N; 1.
DR Pfam; PF05746; DALR_1; 1.
DR Pfam; PF00750; tRNA-synt_1d; 1.
DR PRINTS; PR01038; TRNASYNTHARG.
DR SMART; SM01016; Arg_tRNA_synt_N; 1.
DR SMART; SM00836; DALR_1; 1.
DR SUPFAM; SSF47323; SSF47323; 1.
DR SUPFAM; SSF55190; SSF55190; 1.
DR TIGRFAMs; TIGR00456; argS; 1.
DR PROSITE; PS00178; AA_TRNA_LIGASE_I; 1.
PE 1: Evidence at protein level;
KW 3D-structure; Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm;
KW Direct protein sequencing; Ligase; Nucleotide-binding;
KW Protein biosynthesis; Reference proteome.
FT CHAIN 1..577
FT /note="Arginine--tRNA ligase"
FT /id="PRO_0000151557"
FT MOTIF 122..132
FT /note="'HIGH' region"
FT HELIX 3..17
FT /evidence="ECO:0007829|PDB:5YYM"
FT STRAND 28..30
FT /evidence="ECO:0007829|PDB:5YYM"
FT STRAND 32..34
FT /evidence="ECO:0007829|PDB:5YYM"
FT STRAND 38..41
FT /evidence="ECO:0007829|PDB:5YYM"
FT HELIX 44..51
FT /evidence="ECO:0007829|PDB:5YYM"
FT HELIX 55..65
FT /evidence="ECO:0007829|PDB:5YYM"
FT TURN 69..71
FT /evidence="ECO:0007829|PDB:5YYM"
FT STRAND 72..78
FT /evidence="ECO:0007829|PDB:5YYM"
FT TURN 79..81
FT /evidence="ECO:0007829|PDB:5YYM"
FT STRAND 82..87
FT /evidence="ECO:0007829|PDB:5YYM"
FT HELIX 89..101
FT /evidence="ECO:0007829|PDB:5YYM"
FT TURN 103..106
FT /evidence="ECO:0007829|PDB:5YYM"
FT STRAND 114..118
FT /evidence="ECO:0007829|PDB:5YYM"
FT HELIX 130..132
FT /evidence="ECO:0007829|PDB:5YYM"
FT HELIX 133..148
FT /evidence="ECO:0007829|PDB:5YYM"
FT STRAND 152..157
FT /evidence="ECO:0007829|PDB:5YYM"
FT HELIX 165..177
FT /evidence="ECO:0007829|PDB:5YYM"
FT TURN 178..180
FT /evidence="ECO:0007829|PDB:5YYM"
FT HELIX 189..200
FT /evidence="ECO:0007829|PDB:5YYM"
FT HELIX 204..219
FT /evidence="ECO:0007829|PDB:5YYM"
FT HELIX 222..245
FT /evidence="ECO:0007829|PDB:5YYM"
FT HELIX 252..254
FT /evidence="ECO:0007829|PDB:5YYM"
FT HELIX 258..264
FT /evidence="ECO:0007829|PDB:5YYM"
FT HELIX 265..274
FT /evidence="ECO:0007829|PDB:5YYM"
FT STRAND 278..281
FT /evidence="ECO:0007829|PDB:5YYM"
FT STRAND 284..293
FT /evidence="ECO:0007829|PDB:5YYM"
FT STRAND 297..305
FT /evidence="ECO:0007829|PDB:5YYM"
FT HELIX 313..326
FT /evidence="ECO:0007829|PDB:5YYM"
FT STRAND 331..335
FT /evidence="ECO:0007829|PDB:5YYM"
FT HELIX 339..341
FT /evidence="ECO:0007829|PDB:5YYM"
FT HELIX 342..355
FT /evidence="ECO:0007829|PDB:5YYM"
FT STRAND 360..362
FT /evidence="ECO:0007829|PDB:4OBY"
FT STRAND 364..366
FT /evidence="ECO:0007829|PDB:5YYM"
FT STRAND 377..379
FT /evidence="ECO:0007829|PDB:5YYM"
FT STRAND 383..385
FT /evidence="ECO:0007829|PDB:5YYM"
FT HELIX 390..407
FT /evidence="ECO:0007829|PDB:5YYM"
FT HELIX 414..434
FT /evidence="ECO:0007829|PDB:5YYM"
FT HELIX 446..449
FT /evidence="ECO:0007829|PDB:5YYM"
FT STRAND 452..456
FT /evidence="ECO:0007829|PDB:5YYM"
FT HELIX 457..470
FT /evidence="ECO:0007829|PDB:5YYM"
FT HELIX 473..475
FT /evidence="ECO:0007829|PDB:5YYM"
FT HELIX 478..483
FT /evidence="ECO:0007829|PDB:5YYM"
FT HELIX 491..500
FT /evidence="ECO:0007829|PDB:5YYM"
FT HELIX 503..513
FT /evidence="ECO:0007829|PDB:5YYM"
FT HELIX 517..536
FT /evidence="ECO:0007829|PDB:5YYM"
FT STRAND 539..541
FT /evidence="ECO:0007829|PDB:5B63"
FT HELIX 545..568
FT /evidence="ECO:0007829|PDB:5YYM"
SQ SEQUENCE 577 AA; 64683 MW; C107D346660CF9B8 CRC64;
MNIQALLSEK VRQAMIAAGA PADCEPQVRQ SAKVQFGDYQ ANGMMAVAKK LGMAPRQLAE
QVLTHLDLNG IASKVEIAGP GFINIFLDPA FLAEHVQQAL ASDRLGVATP EKQTIVVDYS
APNVAKEMHV GHLRSTIIGD AAVRTLEFLG HKVIRANHVG DWGTQFGMLI AWLEKQQQEN
AGEMELADLE GFYRDAKKHY DEDEEFAERA RNYVVKLQSG DEYFREMWRK LVDITMTQNQ
ITYDRLNVTL TRDDVMGESL YNPMLPGIVA DLKAKGLAVE SEGATVVFLD EFKNKEGEPM
GVIIQKKDGG YLYTTTDIAC AKYRYETLHA DRVLYYIDSR QHQHLMQAWA IVRKAGYVPE
SVPLEHHMFG MMLGKDGKPF KTRAGGTVKL ADLLDEALER ARRLVAEKNP DMPADELEKL
ANAVGIGAVK YADLSKNRTT DYIFDWDNML AFEGNTAPYM QYAYTRVLSV FRKAEIDEEQ
LAAAPVIIRE DREAQLAARL LQFEETLTVV AREGTPHVMC AYLYDLAGLF SGFYEHCPIL
SAENEEVRNS RLKLAQLTAK TLKLGLDTLG IETVERM
