ID   SYR_LACAC               Reviewed;         561 AA.
AC   Q5FIQ9;
DT   27-JUN-2006, integrated into UniProtKB/Swiss-Prot.
DT   01-MAR-2005, sequence version 1.
DT   03-AUG-2022, entry version 93.
DE   RecName: Full=Arginine--tRNA ligase {ECO:0000255|HAMAP-Rule:MF_00123};
DE            EC=6.1.1.19 {ECO:0000255|HAMAP-Rule:MF_00123};
DE   AltName: Full=Arginyl-tRNA synthetase {ECO:0000255|HAMAP-Rule:MF_00123};
DE            Short=ArgRS {ECO:0000255|HAMAP-Rule:MF_00123};
GN   Name=argS {ECO:0000255|HAMAP-Rule:MF_00123}; OrderedLocusNames=LBA1600;
OS   Lactobacillus acidophilus (strain ATCC 700396 / NCK56 / N2 / NCFM).
OC   Bacteria; Firmicutes; Bacilli; Lactobacillales; Lactobacillaceae;
OC   Lactobacillus.
OX   NCBI_TaxID=272621;
RN   [1]
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=ATCC 700396 / NCK56 / N2 / NCFM;
RX   PubMed=15671160; DOI=10.1073/pnas.0409188102;
RA   Altermann E., Russell W.M., Azcarate-Peril M.A., Barrangou R., Buck B.L.,
RA   McAuliffe O., Souther N., Dobson A., Duong T., Callanan M., Lick S.,
RA   Hamrick A., Cano R., Klaenhammer T.R.;
RT   "Complete genome sequence of the probiotic lactic acid bacterium
RT   Lactobacillus acidophilus NCFM.";
RL   Proc. Natl. Acad. Sci. U.S.A. 102:3906-3912(2005).
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=ATP + L-arginine + tRNA(Arg) = AMP + diphosphate + L-arginyl-
CC         tRNA(Arg); Xref=Rhea:RHEA:20301, Rhea:RHEA-COMP:9658, Rhea:RHEA-
CC         COMP:9673, ChEBI:CHEBI:30616, ChEBI:CHEBI:32682, ChEBI:CHEBI:33019,
CC         ChEBI:CHEBI:78442, ChEBI:CHEBI:78513, ChEBI:CHEBI:456215;
CC         EC=6.1.1.19; Evidence={ECO:0000255|HAMAP-Rule:MF_00123};
CC   -!- SUBUNIT: Monomer. {ECO:0000255|HAMAP-Rule:MF_00123}.
CC   -!- SUBCELLULAR LOCATION: Cytoplasm {ECO:0000255|HAMAP-Rule:MF_00123}.
CC   -!- SIMILARITY: Belongs to the class-I aminoacyl-tRNA synthetase family.
CC       {ECO:0000255|HAMAP-Rule:MF_00123}.
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DR   EMBL; CP000033; AAV43415.1; -; Genomic_DNA.
DR   RefSeq; WP_003548463.1; NC_006814.3.
DR   RefSeq; YP_194446.1; NC_006814.3.
DR   AlphaFoldDB; Q5FIQ9; -.
DR   SMR; Q5FIQ9; -.
DR   STRING; 272621.LBA1600; -.
DR   PRIDE; Q5FIQ9; -.
DR   EnsemblBacteria; AAV43415; AAV43415; LBA1600.
DR   GeneID; 56943165; -.
DR   KEGG; lac:LBA1600; -.
DR   PATRIC; fig|272621.13.peg.1520; -.
DR   eggNOG; COG0018; Bacteria.
DR   HOGENOM; CLU_006406_6_1_9; -.
DR   OMA; NKPLHLG; -.
DR   BioCyc; LACI272621:G1G49-1562-MON; -.
DR   Proteomes; UP000006381; Chromosome.
DR   GO; GO:0005737; C:cytoplasm; IEA:UniProtKB-SubCell.
DR   GO; GO:0004814; F:arginine-tRNA ligase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0005524; F:ATP binding; IEA:UniProtKB-UniRule.
DR   GO; GO:0006420; P:arginyl-tRNA aminoacylation; IEA:UniProtKB-UniRule.
DR   CDD; cd00671; ArgRS_core; 1.
DR   Gene3D; 3.30.1360.70; -; 1.
DR   Gene3D; 3.40.50.620; -; 1.
DR   HAMAP; MF_00123; Arg_tRNA_synth; 1.
DR   InterPro; IPR001278; Arg-tRNA-ligase.
DR   InterPro; IPR005148; Arg-tRNA-synth_N.
DR   InterPro; IPR036695; Arg-tRNA-synth_N_sf.
DR   InterPro; IPR035684; ArgRS_core.
DR   InterPro; IPR008909; DALR_anticod-bd.
DR   InterPro; IPR014729; Rossmann-like_a/b/a_fold.
DR   InterPro; IPR009080; tRNAsynth_Ia_anticodon-bd.
DR   PANTHER; PTHR11956; PTHR11956; 1.
DR   Pfam; PF03485; Arg_tRNA_synt_N; 1.
DR   Pfam; PF05746; DALR_1; 1.
DR   Pfam; PF00750; tRNA-synt_1d; 1.
DR   PRINTS; PR01038; TRNASYNTHARG.
DR   SMART; SM01016; Arg_tRNA_synt_N; 1.
DR   SMART; SM00836; DALR_1; 1.
DR   SUPFAM; SSF47323; SSF47323; 1.
DR   SUPFAM; SSF55190; SSF55190; 1.
DR   TIGRFAMs; TIGR00456; argS; 1.
PE   3: Inferred from homology;
KW   Aminoacyl-tRNA synthetase; ATP-binding; Cytoplasm; Ligase;
KW   Nucleotide-binding; Protein biosynthesis; Reference proteome.
FT   CHAIN           1..561
FT                   /note="Arginine--tRNA ligase"
FT                   /id="PRO_0000242034"
FT   MOTIF           119..129
FT                   /note="'HIGH' region"
SQ   SEQUENCE   561 AA;  63314 MW;  B08F37DFBB05D8B8 CRC64;
     MDFKNEVVDL VSSQVDLPKE KITALIERPK NAKMGDYAFP AFILAKTMHK NPAIIAKDIA
     ENLNSDNFAN IQAVGPYVNF AIDHEKLIAS TLKDVLAEKE HYGDQKLGEG NVPIDMSSPN
     IAKPMSMGHL RSTVIGNSIA KTMKKVGYTP IKINYLGDYG TQFGKLIAAY KHWGNEEDVK
     KDPIMSLFHY YVKFHKEAEN NPELDNEGRE WFKKLEDGDP EAVELWKWFR EVSLKDFKRI
     YKELGVTFDS YNGEAFFNDK MQPVIDELKD KGLLHESRGA QVVDMGEDEN PAIIVKSDGT
     SIYLTRDLAA AEWRMKEYNF VKMLYVVGNE QAQHFVELKT VLKKMGYDWA DEIHHVPFGL
     ITQGGKKLST RKGNVVFLDQ VLRDAVNLAK KQIQEKNPDL ADQDQVAHDV GVGAVVFHDL
     KNDRLDNFDF DLDEVVRFEG DTGPYVQYTN ARAQSVLRKA AAMGEKPSEG DFNINDDWAF
     AVAKDLADFP RIVARSAEKF EPSVIAKFAL DLAKKFNKYY ANVKILTKDD QIGARLALVQ
     ATSIVLTESL RLLGVNAPKE M